Molecularly Imprinted Chalcone-Branched Polyimide-Based Chemosensors with Stripe Nanopatterns for the Detection of Melittin

Abstract

In this study, a chalcone-branched polyimide (CB-PI) was synthesized by the Steglich esterification reaction for selective recognition of the toxic peptide melittin (MEL). MEL was immobilized on a nanopatterned poly(dimethylsiloxane) (PDMS) mold using a conventional surface modification technique to increase binding sites. A stripe-nanopatterned thin CB-PI film was formed on a quartz crystal (QC) substrate by simultaneously performing microcontact printing and ultraviolet (UV) light dimerization using a MEL-immobilized mold. The surface morphology changes and dimensions of the molecularly imprinted polymer (MIP) films with stripe nanopatterns (S-MIP) were analyzed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The sensing signals (Δf and Q e ) of the S-MIP sensor were investigated upon adsorption in a 100-μL dilute plasma solution containing 30 μg/ mL MEL, and its reproducibility, reuse, stability, and durability were investigated. The S-MIP sensor showed high sensitivity (5.49 mL/mg) and coefficient of determination (R 2 = 0.999), and the detection limit (LOD) and the quantification limit (LOQ) were determined as 0.3 and 1.1 μg/mL, respectively. In addition, the selectivity coefficients (k*) calculated from the selectivity tests were 2.7−5.7, 2.1−4.3, and 2.8− 4.6 for bovine serum albumin (BSA), immunoglobulin G (IgG), and apamin (APA), respectively. Our results indicate that the nanopatterned MIP sensors based on CB-PI demonstrate great potential as a sensing tool for the quantitative analysis of biomolecules.

Ultra-Sensitive Portable Visual Paper-Based Viral Molecularly Imprinted Sensor without Autofluorescence Interference

“…Molecular imprinting is an important biomimetic recognition technology that is used to simulate the specific recognition of antibodies and enzymes. Because this strategy is predictable, identifiable, and practical, it is widely used in chromatographic separation, solid-phase extraction, bionic sensing, simulated enzyme catalysis, clinical drug analysis, and other fields. − Preparation of molecularly imprinted polymer (MIP) sensors mainly involves three steps: (a) polymerization of template molecules and functional monomers on substrate materials and (b) elution of the template molecules to obtain MIPs with specific imprinted cavities. (c) The MIPs are specifically bound to the target molecule, the signal changes before and after binding to the target are recorded, and the quantitative equation is established.…”