Molecularly Imprinted Aptamers of Gold Nanoparticles for the Enzymatic Inhibition and Detection of Thrombin

Liao, Yu-Ju; Shiang, Yen-Chun; Huang, Chih‐Ching; Chang, Huan‐Tsung

doi:10.1021/la204651t

Cited by 46 publications

(34 citation statements)

References 77 publications

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“…[12, 13, 15-18] Although gold nanoparticles may enable LSPR spectroscopy and improve sensitivity, they have so far been used as a layer underneath or on top of a molecularly imprinted polymer (MIP) film. [13, 17] In these configurations, nanoparticles are not used as direct transduction elements but for enhancing Raman scattering from analyte molecules (SERS) or propagating SPR on planar gold surface.…”

Section: Introductionmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

101

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The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein we describe a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane co-polymerization. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. We expect that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.

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Section: Introductionmentioning

confidence: 99%

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Abbas

Tian

Morrissey

et al. 2012

Adv Funct Materials

101

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“…Nevertheless, it provides an alternative method to create aptamer‐MIPs. Aside from these copolymerization approaches, imprinting with thiol‐modified aptamers on the surfaces of gold nanoparticles for thrombin binding, was also reported …”

Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

Molecular Imprinting with Functional DNA

Zhang

Liu

2019

Small

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Molecular imprinting refers to templated polymerization with rationally designed monomers, and this is a general method to prepare stable and cost‐effective ligands. This attractive concept however suffers from low affinity, low specificity, and limited signaling mechanisms for binding. Acrydite‐modified DNA oligonucleotides can be readily copolymerized into acrylic polymers. With molecular recognition and catalytic functions, such functional DNAs are recently shown to enhance the performance of molecularly imprinted polymers (MIPs) in a few ways. First, DNA aptamers are used as macromonomers to enhance binding affinity and specificity of MIPs. Second, DNA can help produce optical signals to follow binding events. Third, imprinting can also improve the performance of catalytic DNA by enhancing its activity and specificity toward the template substrate. Finally, MIP is shown to help aptamer selection. Bulk imprinting, nanoparticle imprinting, and surface imprinting are all demonstrated with DNA. Since both DNA and synthetic polymers are cost effective and stable, their hybrid materials still possess such properties while enhancing the function of each component. This review covers recent developments on the abovementioned aspects of DNA‐containing MIPs, a field just emerged in the last five years, and future research directions are discussed toward the end.

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“…It is also possible to target both exosites 1 and 2 by directly coupling the mixture of TBA 15 and TBA 29 on the surface of AuNPs for thrombin inhibition . In addition, by tuning the thymine linker length (T n , n =0–60) and stem pair number (P m , m =0–16) on the terminal end of TBAs, Hsu et al.…”

Section: Anticoagulation Aptamer–aunpsmentioning

confidence: 99%

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

Wang

et al. 2016

ChemBioChem

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Gold nanoparticles (AuNPs) are useful for diagnostic and biomedical applications, mainly because of their ease in preparation and conjugation, biocompatibility, and size-dependent optical properties. However, bare AuNPs do not possess specificity for targets. AuNPs conjugated with DNA aptamers offer specificity for various analytes, such as proteins and small molecules/ions. Although DNA aptamers themselves have therapeutic and target-recognizing properties, they are susceptible to degradation in vivo. When DNA aptamers are conjugated to AuNPs, their stability and cell uptake efficiency both increase, making aptamer-AuNPs suitable for biomedical applications. Additionally, drugs can be efficiently conjugated with DNA aptamer-AuNPs to further enhance their therapeutic efficiency. This review focuses on the applications of DNA aptamer-based AuNPs in several biomedical areas, including anticoagulation, anticancer, antibacterial, and antiviral applications.

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Molecularly Imprinted Aptamers of Gold Nanoparticles for the Enzymatic Inhibition and Detection of Thrombin

Cited by 46 publications

References 77 publications

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Hot Spot‐Localized Artificial Antibodies for Label‐Free Plasmonic Biosensing

Molecular Imprinting with Functional DNA

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

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