Molecularly controlled functional architectures

Sinner, Eva‐Kathrin; Ritz, Sandra; Wang, Yi; Dostálek, Jakub; Jonas, Ulrich; Knoll, Wolfgang

doi:10.1016/s1369-7021(10)70059-6

Cited by 20 publications

(10 citation statements)

References 35 publications

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“…[285][286][287] They aim at significantly reducing the complexity of the biological system while preserving the key characteristics, especially lateral fluidity and sealing properties between intra-and extracellular space. Among various membrane architectures, tethered lipid bilayer membranes have been recognized as a powerful platform in biomimetics [288] and for the construction of biosensors.…”

Section: Introductionmentioning

confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

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This thesis focuses on the controlled assembly of monodisperse polymer colloids into ordered two-dimensional arrangements. These assemblies, commonly referred to as colloidal monolayers, are subsequently used as masks for the generation of arrays of complex metal nanostructures on solid substrates.The motivation of the research presented here is twofold. First, monolayer crystallization methods were developed to simplify the assembly of colloids and to produce more complex arrangements of colloids in a precise way. Second, various approaches to colloidal lithography are designed with the aim to include novel features or functions to arrays of metal nanostructures.The air/water interface was exploited for the crystallization of colloidal monolayer architectures as it combines a two-dimensional confinement with a high lateral mobility of the colloids that is beneficial for the creation of high long range order. A direct assembly of colloids is presented that provides a cheap, fast and conceptually simple methodology for the preparation of ordered colloidal monolayers. The produced two-dimensional crystals can be transformed into nonclose-packed architectures by a plasma-induced size reduction step, thus providing valuable masks for more sophisticated lithographic processes. Finally, the controlled co-assembly of binary colloidal crystals with defined stoichiometries on a Langmuir trough is introduced and characterized with respect to accessible configurations and size ratios.Several approaches to lithography are presented that aim at introducing different features to colloidal lithography. First, using metal-complex containing latex particles, the synthesis of which is described as well, symmetric arrays of metal nanoparticles can be created by controlled combustion of the organic material of the colloids. The process does not feature an inherent limit in nanoparticle size and is able to produce complex materials as will be demonstrated for FePt alloy particles. Precise control over both size and spacing of the particle array is presented.Second, two lithographic processes are introduced to create sophisticated nanoparticle dimer units consisting of two crescent shaped nanostructures in close proximity; essentially by using a single colloid as mask to generate two structures simultaneously. Strong coupling processes of the parental plasmon resonances of the two objects are observed that are accompanied by high near-field enhancements. A plasmon hybridization model is elaborated to explain all polarization dependent shifts of the resonance positions. Last, a technique to produce laterally patterned, ultra-flat substrates without surface topographies by embedding gold nanoparticles in a silicon dioxide matrix is applied to construct robust and re-usable sensing architectures and to introduce an approach for the nanoscale patterning of solid supported lipid bilayer membranes.

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Section: Introductionmentioning

confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

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“…Membrane proteins exert a variety of fundamental functions such as electron and proton transfer, voltage‐gated ion translocation, and enzymatic transformations. Since most of these processes can be monitored and controlled by electrochemical techniques, substantial efforts have been made to develop strategies for immobilizing membrane proteins on electrodes while preserving their native functions 1–4. In one of the most interesting approaches tethered membranes are used to immobilize functional integral proteins on an electrode surface 4–8.…”

mentioning

confidence: 99%

“…Since most of these processes can be monitored and controlled by electrochemical techniques, substantial efforts have been made to develop strategies for immobilizing membrane proteins on electrodes while preserving their native functions 1–4. In one of the most interesting approaches tethered membranes are used to immobilize functional integral proteins on an electrode surface 4–8. One strategy is to first attach a solubilized protein through a His tag to an appropriately functionalized metal surface, and then reconstitute a lipid bilayer around the fixed protein by detergent–lipid exchange 5.…”

mentioning

confidence: 99%

A remote sensing contribution to hydrologic modelling in arid and inaccessible watersheds, Pishin Lora basin, Pakistan

Sagin

Sultan

Khan

et al. 2011

Hydrological Processes

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Abstract:The lack of adequate field measurements often hampers the construction and calibration of rainfall-runoff models over many of the world's watersheds. We adopted methodologies that rely heavily on readily available remote sensing datasets as viable alternatives for assessing, managing, and modelling of such remote and inadequately gauged regions. The Soil and Water Assessment Tool was selected for continuous (1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005) rainfall-runoff modelling of one such area, the northeast part of the Pishin Lora basin (NEPL). Input to the model included satellite-based Tropical Rainfall Measuring Mission precipitation data, and modelled runoff was calibrated against satellite-based observations, the latter included: (i) monthly estimates of the water volumes impounded by the Khushdil Khan (latitude 30°40 0 N, longitude 67°40 0 E), and the Kara Lora (latitude 30°34 0 N, longitude 66°52 0 E) reservoirs, and (ii) inferred wet versus dry conditions in streams across the NEPL. Calibrations were also conducted against observed flow reported from the Burj Aziz Khan station at the NEPL outlet (latitude 30°20 0 N; longitude 66°35 0 E). Model simulations indicate that (i) average annual precipitation (1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005), runoff and recharge in the NEPL are 1300 ð 10 6 m 3 , 148 ð 10 6 m 3 , and 361 ð 10 6 m 3 , respectively; (ii) within the NEPL watershed, precipitation and runoff are high for the northeast (precipitation: 194 mm/year; runoff: 38 ð 10 6 m 3 /year) and northwest (134 mm/year; 26 ð 10 6 m 3 /year) basins compared to the southern basin (124 mm/year; 8 ð 10 6 m 3 /year); and (3) construction of delay action dams in the northeast and northwest basins could increase recharge from 361 ð 10 6 m 3 /year up to 432 ð 10 6 m 3 /year and achieve sustainable extraction. The adopted methodologies are not a substitute for traditional approaches, but they could provide first-order estimates for rainfall, runoff, and recharge in the arid and semi-arid parts of the world that are inaccessible and/or lack adequate coverage with field data.

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“…Supported membranes, which comprise a bilayer assembly of phospholipids on a solid substrate, enable the investigation of the protein and membrane using a wide range of surface-sensitive analytical techniques. [14][15][16] In general, the self-assembly properties of S-layer structures permit their easy preparation on solid supports to form a tether for the lipid bilayer structure via vesicle fusion 14,15 which we call the S-layer supported lipid membrane (SsLM). [7][8][9] S-layer proteins do recrystallize as a crystalline monomolecular layer on a broad spectrum of solid substrates, including gold-and silicon dioxide-coated surfaces, in order to form a 5-10 nm-thick highly porous lattice.…”

Section: Introductionmentioning

confidence: 99%

Inspired and stabilized by nature: ribosomal synthesis of the human voltage gated ion channel (VDAC) into 2D-protein-tethered lipid interfaces

et al. 2015

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We present an elegant synthesis and reconstitution approach for functional studies of voltage responsive membrane proteins. For such studies, we propose a planar architecture of an S-layer-supported lipid membrane as a suitable matrix for presenting unmodified membrane protein species, and here we focus on the voltage-dependent anion channel (VDAC) from human mitochondria. The presented cell-free strategy, in which VDAC proteins are synthesized in bacterial cell lysate, into a membrane structure, offers a great advantage in the study of such subtle membrane proteins over the conventional, cell-based synthesis approach in terms of reproducibility. The material-assay combination is superior over cell-culture related synthesis and purification approaches as here we bypass by a one-step synthesis procedure the complex cell culture, and expression and purification endeavours, and, moreover, the protein of interest never has to be detergent solubilized and had been synthesized de novo. We provide here a detailed description from the all over procedure and our first results, describing in detail the cell-free synthesis and robustness of such a material-assay combination: functional VDAC protein species embedded in a planar membrane architecture and ready for electrochemical characterization.

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Molecularly controlled functional architectures

Cited by 20 publications

References 35 publications

Surface Patterning with Colloidal Monolayers

Surface Patterning with Colloidal Monolayers

A remote sensing contribution to hydrologic modelling in arid and inaccessible watersheds, Pishin Lora basin, Pakistan

Inspired and stabilized by nature: ribosomal synthesis of the human voltage gated ion channel (VDAC) into 2D-protein-tethered lipid interfaces

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