Molecular weight of scleroglucan and other extracellular microbial polysaccharides by size-exclusion chromatography and low angle laser light scattering

Lecacheux, D.; Mustiere, Y.; Panaras, R.; Brigand, G.

doi:10.1016/0144-8617(86)90005-6

Cited by 57 publications

(11 citation statements)

References 19 publications

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“…23 This biopolymer is chemically identical 24 to the schizophyllum polysaccharide ͑schizophyllan͒ used by Sasaki 15 but typically has a larger molecular weight of around 5 ϫ 10 6 as opposed to roughly 5 ϫ 10 5 for schizophyllan. 25,26 The polydispersity of typical scleroglucan preparations is low ͑approximately 1.1͒ due to fermentation-based production methods. 25 This polymer was chosen for both its low cost and physical properties.…”

Section: Polymer Properties and Nondimensional Parametersmentioning

confidence: 99%

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An experimental and numerical investigation of drag reduction in a turbulent boundary layer using a rigid rodlike polymer

et al. 2005

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The drag reduction in a zero pressure gradient (ZPG) turbulent boundary layer (TBL) using a rigid rodlike polymer was experimentally and numerically investigated. Using injection of the rigid polysaccharide scleroglucan, drag reductions of approximately 10–15 % were observed, with three distinct drag reduction regimes: a non-Newtonian flow region near the injector, followed by a region of nearly constant drag reduction, and finally a region of negligible drag reduction. Decreasing the effective rotary Peclét number reduced the drag reduction effectiveness. Increasing the concentration did not improve the drag reduction, but instead shifted the spatial development of the drag reduction further downstream. A complementary direct numerical simulation of the ZPG TBL using the rigid rod constitutive equation was performed at a matching inlet Reynolds number. The simulation assumed a homogeneous concentration distribution and used estimated effective parameters for the rodlike additive. Spatial evolution of the fiber stresses is rapid and develops asynchronously with the flow structure. The simulated turbulence statistics and experimental measurements at a position 23 boundary layer thicknesses downstream compare favorably, with the primary differences due to the concentration distribution assumed in the simulation.

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Section: Polymer Properties and Nondimensional Parametersmentioning

confidence: 99%

“…25,26 The polydispersity of typical scleroglucan preparations is low ͑approximately 1.1͒ due to fermentation-based production methods. 25 This polymer was chosen for both its low cost and physical properties. We will refer to the polymer with the abbreviation "SG" throughout this work.…”

Section: Polymer Properties and Nondimensional Parametersmentioning

confidence: 99%

An experimental and numerical investigation of drag reduction in a turbulent boundary layer using a rigid rodlike polymer

et al. 2005

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“…Variations in branching length have been previously reported among scleroglucans, but the incidence of cultivation parameters on this feature is still poorly understood and optimizations usually follow an empirical basis (Lecacheux et al. 1986; Morin 1998; Laroche and Michaud 2007).…”

Section: Discussionmentioning

confidence: 99%

Structural stability ofSclerotium rolfsiiATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach

Fariña

Viñarta

Cattaneo

et al. 2009

Journal of Applied Microbiology

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Aims: Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter‐scale production led to productivity (Pr) and yield (YP/C) values higher at 48 h (Pr = 0·542 g l−1 h−1; YP/C = 0·74) than at 72 h (Pr = 0·336 g l−1 h−1; YP/C = 0·50). Both EPSs were neutral glucose‐homopolysaccharides with a β‐(1,3)‐glycosidic backbone and single β‐(1,6)‐glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra‐red diagnostic peak at 890 cm−1 confirmed β‐glycosidic linkages, while gentiobiose released by β‐(1,3)‐glucanases confirmed single β‐1,6‐glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.

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“…16 Since perfect linearity of the LALLS signal area vs. the injected quantity was observed within the range of SEC concentrations, A , was neglected. Taken together, the results which were progressively accumulated ( Table I is the collection of 10 runs over the past 6 months) have allowed us to show a very simple relationship between the absolute a, (from LALLS) and the same average in polystyrene units aPs:…”

Section: Characterization Of Polyorganophosphazenesmentioning

confidence: 99%

High performance size exclusion chromatography of poly(organo)phosphazenes

Jaeger

Lecacheux²,

Potin³

1990

J of Applied Polymer Sci

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SynopsisThe determination of the molecular weights of polyorganophosphazenes can only be achieved with great care. The nature of the substituents, the proportion of residual chlorine atoms after substitution, and also any traces of hydrolysis can considerably modify the behavior of these polymers in solution and thus lead to erroneous results. In our current research we have been developing a characterization method based on steric exclusion chromatography, coupled with a light scattering detector. We have used columns with styrene-divinylbenzene organic micropacking as the stationary phase and THF with added LiBr (0.1 mol/L) as the eluent. This method has enabled us to establish accurate correlations between molecular weight, hydrodynamic volume, and viscosimetric measurements for polyaryloxyphosphazenes of varying structures and origins. The method also provides results which agree very well with the variables for the preparation of polyphosphazenes, which is based on polycondensation of P-trichloro-N-dichlorophosphoryl monophosphazene C1,P = N-P(O)Cl,.Finally this method allows us to show that, despite their mineral backbone, these polymers obey Benoit's universal calibration concept.

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Molecular weight of scleroglucan and other extracellular microbial polysaccharides by size-exclusion chromatography and low angle laser light scattering

Cited by 57 publications

References 19 publications

An experimental and numerical investigation of drag reduction in a turbulent boundary layer using a rigid rodlike polymer

An experimental and numerical investigation of drag reduction in a turbulent boundary layer using a rigid rodlike polymer

Structural stability ofSclerotium rolfsiiATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach

High performance size exclusion chromatography of poly(organo)phosphazenes

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