Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels

Conversion of bovine prekallikrein to kallikrein. Evidence of limited proteolysis of prekallikrein by bovine hageman factor (factor XII)

“…Bovine Hageman factor was purified by the method of Komiya et al [7], following its potency to activate prekallikrein, and the preparation was characterized by determining its ability to correct the coagulation defect of Hageman factor-deficient human plasma [7]. SDS-polyacrylamide gel disc electrophoresis was performed by the method of Shapiro et al [8]. Gels were stained with 0.2 '% Coomassie Brilliant Blue in methanol-acetic acid-water (5: 1 :5, v/v/v) and excess dye was removed by dialysis against 7.5 5% acetic acid-5 5% methanol solution [o].…”

Cell envelope proteins of dividing and non‐dividing cells of Escherichia coli

“…To determine approximately the molecular weight of the protein present only in membrane fraction of dividing cells, the technique of Shapiro, Viiiuela and Maize1 [9] was applied to urea-acetic acid polyacrylamide gels. Proteins of known molecular weight were dissolved in phenol-acetic acid mixture and added directly to the samples of envelope protein fraction, Samples without added protein standard were run in parallel and served as control.…”

The molecular weight of a yeast pyruvate kinase and its subunits

“…Electrophoretic separation on 7% and 10% polyacrylamide gels containing SDS (0.1%) at pH 7.0 was performed by a modification of the method of Shapiro et al [9] .…”