“…Bovine Hageman factor was purified by the method of Komiya et al [7], following its potency to activate prekallikrein, and the preparation was characterized by determining its ability to correct the coagulation defect of Hageman factor-deficient human plasma [7]. SDS-polyacrylamide gel disc electrophoresis was performed by the method of Shapiro et al [8]. Gels were stained with 0.2 '% Coomassie Brilliant Blue in methanol-acetic acid-water (5: 1 :5, v/v/v) and excess dye was removed by dialysis against 7.5 5% acetic acid-5 5% methanol solution [o].…”