Molecular weight distribution of wood components extracted from Pinus taeda biotreated by Ceriporiopsis subvermispora

Guerra, Anderson; Mendonça, Regis Teixeira; Ferraz, André

doi:10.1016/s0141-0229(03)00099-1

Cited by 73 publications

(49 citation statements)

References 27 publications

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“…These results indicate that ␤-1, ␤-5, ␤-␤, and 4-O-5 substructures were more resistant to the biological attack and that arylglycerol-␤-O-aryl units were degraded, resulting in a depolymerized residual lignin. A previous study with these MWLs also showed a similar trend, since the MWL average molecular mass decreased with biodegradation time (15).…”

Section: Discussionsupporting

confidence: 69%

“…On the other hand, some white-rot fungi degrade lignin efficiently without producing LiP (20). One of them, Ceriporiopsis subvermispora, has been extensively studied (15,16,17,31,32), owing to its suitability for biopulping (1,27). Although the industrial applicability of this fungus has been recognized, its ligninolytic strategies are not completely understood.…”

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confidence: 99%

“…Lignin depolymerization has been demonstrated in studies based on the molecular mass distribution of milled wood lignins (MWLs) recovered from biotreated wood samples (15) and in studies using in situ derivatization followed by reductive cleavage (DFRC) of lignin in biotreated wood, which indicates lignin depolymerization as a consequence of aryl-ether degradation (16).…”

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confidence: 99%

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Structural Characterization of Lignin during Pinus taeda Wood Treatment with Ceriporiopsis subvermispora

Guerra¹,

Mendonça²,

Ferraz³

et al. 2004

Appl Environ Microbiol

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Although lignin is recalcitrant, some specialized fungi developed the ability to degrade this complex molecule. Most of these fungi belong to the basidiomycetes and are responsible for the white-rot decay process (19). White-rot fungi use an intricate oxidative complex to degrade lignin, which is based on oxidative enzymes and low-molecular-mass mediators (28). Several white-rot fungi produce a lignin peroxidase (LiP) that can initiate one-electron oxidation of nonphenolic arylglycerol-␤-O-aryl ether units. This oxidation is followed by C␣-C␤ or ␤-O-aryl cleavage to give aromatic aldehydes or phenylglycerols, respectively, as primary products (19). On the other hand, some white-rot fungi degrade lignin efficiently without producing LiP (20). One of them, Ceriporiopsis subvermispora, has been extensively studied (15,16,17,31,32), owing to its suitability for biopulping (1,27). Although the industrial applicability of this fungus has been recognized, its ligninolytic strategies are not completely understood. It has often been assumed that lignin degradation by C. subvermispora is dependent on manganese-dependent peroxidases and laccases (28). The occurrence of lignin degradation inside the wood matrix during the first stages of wood decay, when low cell wall permeability does not permit enzyme diffusion, has clearly indicated that some low-molecular-mass agents also act in the early stages of lignin biodegradation (2). Lipid peroxidation induced by manganese-dependent peroxidase has been proposed for lignin biodegradation by C. subvermispora (6,17,18,32). Studies with lignin model compounds have shown that nonphenolic arylglycerol-␤-O-aryl units are disrupted by means of one-electron oxidation mechanisms similar to the routes proposed for basidiomycetes that produce LiP (18,32).Under solid-state fermentation of wood, C. subvermispora extensively depolymerizes lignin. Lignin depolymerization has been demonstrated in studies based on the molecular mass distribution of milled wood lignins (MWLs) recovered from biotreated wood samples (15) and in studies using in situ derivatization followed by reductive cleavage (DFRC) of lignin in biotreated wood, which indicates lignin depolymerization as a consequence of aryl-ether degradation (16).The present study provides additional data for evaluating lignin biodegradation by C. subvermispora during solid-state fermentation of Pinus taeda wood. The structural characteristics of MWLs extracted from sound (untreated) and biotreated wood samples were elucidated using wet-chemical and spectroscopic analyses. MATERIALS AND METHODSFungus, inoculum preparation, and wood biodegradation. C. subvermispora (Pilat) Gilbertson et Ryvarden cultures were maintained on 2% (wt/vol) malt extract agar plates at 4°C. The wood chips (approximately 2.5 by 1.2 by 0.2 cm) used in this work came from a single log of a 28-year-old P. taeda tree.Biodegradation experiments were carried out in 20-liter polypropylene bioreactors. Precolonized wood chips were used as inoculum seed to start the colonization ...

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Section: Discussionsupporting

confidence: 69%

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confidence: 99%

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confidence: 99%

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Structural Characterization of Lignin during Pinus taeda Wood Treatment with Ceriporiopsis subvermispora

Guerra¹,

Mendonça²,

Ferraz³

et al. 2004

Appl Environ Microbiol

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“…78 No entanto, parte da celulose residual foi despolimerizada a partir de 30 dias de cultivo, indicando que ao menos algum nível de degradação deve ocorrer. 79 Essa despolimerização de celulose poderia ser induzida tanto por radicais hidroxila oriundos da reação de Fenton como pela ação de endoglicanases, produzidas pelo fungo. 49,80 A baixa capacidade degradativa da celulose poderia ser atribuída à biossíntese dos ácidos alquil-itacônicos, como postulado pelo grupo de Watanabe, 76,81 ou ainda pela secreção de elevadas quantidades de ácido oxálico no meio, que também pode quelar e indisponibilizar Fe 3+ para as reações de redução por derivados fenólicos e a posterior reação de Fenton.…”

Section: Sistema Fentonunclassified

Mecanismos envolvidos na biodegradação de materiais lignocelulósicos e aplicações tecnológicas correlatas

Aguiar¹,

Ferraz²

2011

Quím. Nova

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Recebido em 9/11/10; aceito em 21/6/11; publicado na web em 8/8/11MECHANISMS INVOLVED IN THE BIODEGRADATION OF LIGNOCELLULOSIC MATERIALS AND RELATED TECHNOLOGICAL APPLICATIONS. The biodegradation of lignocellulosic materials is an important natural process because it is responsible for the carbon recycling. When induced under controlled conditions, this process can be used for technological applications such as biopulping, biobleaching of cellulosic pulps, pre-treatment for subsequent saccharification and cellulosic-ethanol production, and increase of the digestibility in agroindustrial residues used for animal feed. In the present work, the enzymatic and non-enzymatic mechanisms involved in the biodegradation of lignocellulosic materials by fungi were reviewed. Furthermore, the technological applications of these extracellular metabolites are presented and discussed.Keywords: wood biodegradation; oxidative enzymes; Fenton reaction. INTRODUÇÃOA biodegradação dos materiais lignocelulósicos tem sido objeto de muitos estudos, pois, além de corresponder a uma importante etapa do ciclo do carbono na natureza, também pode ser aplicada em processos tecnológicos. As aplicações em questão são variadas e incluem desde a ação direta de fungos sobre a madeira, desenhada como uma etapa de pré-tratamento aos processos de fabricação de celulose e papel, 1,2 até processos que usam os fungos decompositores de materiais lignocelulósicos como agentes indutores da degradação de compostos xenobióticos, quer em solos contaminados 3 ou em efluentes industriais. 4 O pré-tratamento de materiais lignocelulósicos com fungos também tem sido apontado como uma forma de facilitar processos subsequentes de conversão da celulose em açúcares fermentescíveis destinados à bioconversão em etanol e outros produtos de interesse comercial. 5,6 Também na área de nutrição animal, a aplicação de fungos decompositores de materiais lignocelulósicos tem sido descrita. Nesse caso, o pré-tratamento biológico é usado para aumentar a digestibilidade de resíduos agroindustriais utilizados na alimentação de ruminantes e não ruminantes. Os fungos, principalmente os causadores de podridão branca seletivos na degradação de lignina, desestruturam a parede celular vegetal, promovendo a conversão dos polissacarídeos em açúcares de fácil assimilação. Além disso, a biomassa fúngica que se desenvolve sobre o lignocelulósico pode servir como fonte de proteína. 7 A aplicação de enzimas produzidas pelos fungos decompositores de materiais lignocelulósicos também tem ganhado muito interesse na atualidade. O uso de xilanases 8 e lacases 9,10 em processos de branqueamento de polpas celulósicas e a aplicação de celulases na hidrólise enzimática de celulose nos processos de produção de etanol de segunda geração, 11 são exemplos de como o avanço no entendimento da biodegradação dos lignocelulósicos tem permitido explorar aplicações tecnológicas de grande relevância para o mundo moderno.O desenvolvimento das aplicações mencionadas anteriormente tem ocorrido de forma simultânea à ger...

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“…Nevertheless, fungal pre-treatment is accompanied by two intrinsic disadvantages: a long residence time and low degradation efficiency (Dias et al 2010;Pinto et al 2012). Additionally, most white-rot fungi degrade lignin and polysaccharides simultaneously, but some preferentially degrade lignin (Kuhad et al 1997;Guerra et al 2003). In this regard, Phanerochaete chrysosporium is the most widely used white-rot fungus for lignin degradation (Bak et al 2009;Zeng et al 2014;Saha et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Fungus-Assisted Acetic Acid Pre-Treatment of Eucommia ulmoides Oliver Seed Shells for Enhancement of Enzymatic Hydrolysis

Ouyang¹,

Wang²,

Peng³

et al. 2017

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The potential of nine fungal strains for pre-treating Eucommia ulmoides Oliver seed shells (EUOSSs) was investigated. Phanerochaete chrysosporium Burds. was found to be the best fungal strain for pretreating EUOSSs. After co-pre-treatment with acetic acid and P. chrysosporium Burds., which was cultivated in a solid state with an approximately 74% moisture content at 28 °C for 28 d, the weight loss of the EUOSSs was 51.9%. Because of the cooperative efficiency of the biochemical pre-treatment, an enzymatic digestibility value of 86.6% was achieved. The high digestibility value was attributed to the synergism between the acetic acid and fungal treatments, which led to improved enzymatic accessibility of the EUOSSs. As an environmentally friendly processing method, fungal pre-treatment can save a great amount of energy and, in combination with an acetic acid treatment, is more efficient at improving the rate of sugar transformation.

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Molecular weight distribution of wood components extracted from Pinus taeda biotreated by Ceriporiopsis subvermispora

Cited by 73 publications

References 27 publications

Structural Characterization of Lignin during Pinus taeda Wood Treatment with Ceriporiopsis subvermispora

Structural Characterization of Lignin during Pinus taeda Wood Treatment with Ceriporiopsis subvermispora

Mecanismos envolvidos na biodegradação de materiais lignocelulósicos e aplicações tecnológicas correlatas

Fungus-Assisted Acetic Acid Pre-Treatment of Eucommia ulmoides Oliver Seed Shells for Enhancement of Enzymatic Hydrolysis

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