Molecular weight distribution of polysaccharides from edible seaweeds by high-performance size-exclusion chromatography (HPSEC)

Gómez‐Ordóñez, Eva; Jiménez‐Escrig, Antonio; Rupérez, Pilar

doi:10.1016/j.talanta.2012.01.067

Cited by 105 publications

(47 citation statements)

References 30 publications

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“…The samples were shaken overnight and filtered over a 0.45 µm regenerated cellulose filter (GE Healthcare). The detectors were calibrated using a pullulan standard (P-100, Shodex) [11,12]. The mobile phase solvent consisted of a 0.05 M sodium acetate (13.6 g/2 L) and 0.02 wt% sodium azide (0.4 g/2 L) adjusted to pH 6 with acetic acid.…”

Section: Molecular Weight Distributionmentioning

confidence: 99%

Chain-Length Shortening of Methyl Ethyl Hydroxyethyl Cellulose: An Evaluation of the Material Properties and Effect on Foaming Ability

et al. 2018

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During the past century, plastics have become a natural element in our every-day life. Lately however, an awareness about the fossil origin and often non-degradable nature of many plastics is rising. This has resulted in the emergence of some bio-based and/or biodegradable plastics, often produced from renewable resources. One possible candidate for bioplastics production could be found in cellulose. This paper aims at contributing information regarding a cellulose derivative, which could possibly be used in foamed plastics applications. Therefore, the reduction of the chain-length of a methyl ethyl hydroxyethyl cellulose (MEHEC), assessed by size exclusion chromatography, and the effect of chain-length on the foaming behaviour were studied. The foaming was accomplished with a hot-mould technique using aqueous polymer solutions. The generated steam was here used as the blowing agent and important parameters were polymer concentration and solution viscosity. The density of the produced foams was assessed and was in some cases comparable to that of commodity foams. It was found that reducing the chain-length enabled an increase of the initial polymer concentration for the foaming process. This is believed to be beneficial for creating more structurally stable foams of this type.

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Section: Molecular Weight Distributionmentioning

confidence: 99%

Chain-Length Shortening of Methyl Ethyl Hydroxyethyl Cellulose: An Evaluation of the Material Properties and Effect on Foaming Ability

et al. 2018

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“…[11] Alginic acid, fucoidan, and laminarin are three common and stable polysaccharides with large molecular weights in algae. [12][13][14] Rupérez and colleagues [15] already detected the three polysaccharides with different molecular weights (213-277 KD, 105-117 KD, and 7.3-7.6 KD for alginic acid, fucoidan, and laminarin, respectively) by commercial HPSEC. This study selected these three polysaccharides as the target compounds to evaluate the quantitation properties of MSP-based HPSEC and compared the prepared column with the commercial column.…”

Section: Introductionmentioning

confidence: 98%

“…Normally, a salt as an electrolyte is added to the mobile phase to suppress ion exclusion between the ionic polysaccharides and column packing. [15,23,24] NaCl, NaNO 3 , and NaAc as additives were added to the mobile phase and their effects on separation were investigated. The separation of three polysaccharides in the NaCl mobile phase was limited, and the column was corroded seriously by NaCl.…”

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confidence: 99%

“…The separation of three polysaccharides in the NaCl mobile phase was limited, and the column was corroded seriously by NaCl. To avoid the corrosive effects of salt, Rupérez and colleagues [15] applied NaNO 3 in the mobile phase and separated some polysaccharides in HPSEC, but the peaks shape of the targets was broad and not spire-like. The peaks for alginic acid and fucoidan were not separated completely in this study, as shown in Figure 4.…”

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confidence: 99%

“…Some researchers suggested that a low pH and high ionic strength buffer was suitable as a mobile phase for the separation of polysaccharides in HPSEC. [15,23] On the other hand, a lower ionic strength and pH of NaAc buffer solution was suitable for the prepared HPSEC, and perfect separation of the three polysaccharides was obtained. A suitable ionic buffer solution is a key point for a perfect mobile phase for polysaccharides separation in HPSEC, but a strong ionic salt buffer solution is not the only consideration.…”

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Exploration of Mesoporous Siliceous Particle-Based High-Performance Size Exclusion Chromatography for the Quantitation of Biomacromolecular Polysaccharides

Tang

Row

2014

Journal of Liquid Chromatography & Related Technologies

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Based on a modification of normal hydrothermal synthesis, uniform and spherical mesoporous siliceous particles (MSP) were prepared and applied to high-performance size exclusion chromatography (HPSEC). Sodium acetate buffer solution at pH ¼ 4.0 was the optimal mobile phase for the separation of the three biomacromolecular polysaccharides (alginic acid, fucoidan, and laminarin) in the MSP-based HPSEC. Based on the optimal separation conditions, the calibration curves of the three polysaccharide standards showed good linearity (R 2 > 0.999). In addition, the limits of detection of the three standards ranged from 0.02 to 0.13 mg mL À1 , the limits of quantitation ranged from 0.07 to 0.43 mg mL À1 , the related recoveries were 97.7-101.2%, and the relative standard deviations of the retention time were 0.2-0.5. All the quantitative parameters showed that the developed approach was suitable for quantifying biomacromolecular polysaccharides. Compared to a common commercial HPSEC column, the MSP-based HPSEC column was low cost and had a similar separation efficiency for the three polysaccharides. In particular, 2.8 mg g À1 of the alginic acid fractions, 4.4 mg g À1 of the fucoidan fractions, and 6.9 mg g À1 of the laminarin fractions were separated from the Thallus Laminariae extracts by MSP-based HPSEC. Similar results were obtained from the commercial HPSEC.

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Biological Activity of Algal Sulfated and Nonsulfated Polysaccharides

Rupérez

Gómez‐Ordóñez

Jiménez‐Escrig

2013

Bioactive Compounds From Marine Foods

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Molecular weight distribution of polysaccharides from edible seaweeds by high-performance size-exclusion chromatography (HPSEC)

Cited by 105 publications

References 30 publications

Chain-Length Shortening of Methyl Ethyl Hydroxyethyl Cellulose: An Evaluation of the Material Properties and Effect on Foaming Ability

Chain-Length Shortening of Methyl Ethyl Hydroxyethyl Cellulose: An Evaluation of the Material Properties and Effect on Foaming Ability

Exploration of Mesoporous Siliceous Particle-Based High-Performance Size Exclusion Chromatography for the Quantitation of Biomacromolecular Polysaccharides

Biological Activity of Algal Sulfated and Nonsulfated Polysaccharides

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