Molecular Weight Distribution of Hyaluronic Acid of Human Synovial Fluid in Rheumatic Diseases

Bjelle, A; Andersson, Torvald; Granath, Kirsti

doi:10.3109/03009748309102899

Cited by 31 publications

(17 citation statements)

References 17 publications

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“…However, an increase in the level of low molecular weight-HA (LMW-HA) under inflammatory or pathologic conditions, such as osteoarthritis (OA), reduces the viscoelasticity of synovial fluid, leading to deterioration of joint lubrication. 1 In addition, LMW-HA has effects on immune and inflammatory processes. 16 In synovial fluid, the amount of HA and its molecular weight distribution is determined by a balance between the synthesis and degradation of HA.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells

et al. 2010

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Hyaluronan (HA) plays a crucial role in the lubricating and buffering properties of synovial fluid. The purpose of this study was to examine the effects of interleukin (IL)-1beta on HA degradation in cultured synovial membrane cells. The rabbit synovial membrane cell line HIG-82 was cultured with and without IL-1beta. The amounts of HA of varying molecular weights in the medium were analyzed using high-performance liquid chromatography, the mRNA levels of HA synthase (HAS) and hyaluronidase (HYAL) were analyzed by means of real-time PCR, and HYAL activity was analyzed by HA zymography. The amounts of HA with a molecular weight lower than 300 kDa, and between 300 and 1900 kDa, in the culture medium of HIG-82 cells were significantly higher in the presence of IL-1beta. However, the amount of HA with a molecular weight greater than 1900 kDa was significantly lower in the presence of IL-1beta. Both HAS2 and HAS3 mRNA levels were upregulated by treatment with IL-1beta. So, too, were the levels of HYAL1 and HYAL2 mRNA, which resulted in enhanced HYAL activity. However, HYAL activity was inhibited by transfection of HYAL2-siRNA. Our results suggest that IL-1beta is a crucial factor in the fragmentation of HA in inflammatory joints.

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Section: Introductionmentioning

confidence: 99%

“…13 It has been suggested that the degradation of HA by hyaluronidase (HYAL) plays a crucial role in the development of OA. 29 HYAL is a family of b-endoglucosidases that degrades HA into small fragments by cleaving internal b (1,4) linkages. 10 In human, the HYAL genes HYAL1, HYAL2, HYAL3, HYAL4, PH-20, and HYALP1 have been identified.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells

et al. 2010

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“…[2][3][4] Different methods for the estimation of HA concentration and molecular weights have been used. [1][2][3][4][5] The HA has usually been isolated from the synovial fluid before the analyses, which introduces the uncertainty of incomplete recovery and possible degradation of the polymer. Most studies have afforded mean molecular weifhts rather than molecular weight distributions,-3 and in some studies control values were lacking.S In the present work we determined the molecular weight distribution of HA in SF by a gel chromatographic technique developed in one of our laboratories.…”

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confidence: 99%

Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies.

Dahl¹,

Dahl²,

Engström‐Laurent³

et al. 1985

Annals of the Rheumatic Diseases

492

303

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SUMMARY The molecular weight distribution of hyaluronate (HA) in synovial fluid (SF) from 10 patients with rheumatoid arthritis (RA), from six patients with other joint disorders, and from five recently deceased persons without joint affections was investigated by a gel chromatographic procedure. A new and highly specific radioassay was used for determination of the HA concentration in the effluent from the chromatographic column, and this allowed analyses on 0-5 ml or less of untreated synovial fluid. The results confirmed the findings by others that the weight-average molecular weight (Mw) of HA in SF from patients with RA (4.8 x 106) was similar to that in other joint diseases (5.0 x 106) and moderately but significantly (p 6 x 106) is in excess in joint disease. The pathological state is therefore characterised not by lack of high molecular weight hyaluronate but by a dilution of it.

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“…On the other hand, it is well-known that hyaluronan has been detected in immune-related diseases and inflammatory sites [27][28][29] and it induces various kinds of inflammatory factors. 16,30) Therefore, it may regulate both immune and inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Hyaluronan: A Cytokine-Like Factor Present in Rabbit Uterine Cervix during Pregnancy

Uchiyama

Matsumoto

Suzuki

et al. 2004

Biological & Pharmaceutical Bulletin

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Molecular Weight Distribution of Hyaluronic Acid of Human Synovial Fluid in Rheumatic Diseases

Cited by 31 publications

References 17 publications

Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells

Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells

Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies.

Endogenous Hyaluronan: A Cytokine-Like Factor Present in Rabbit Uterine Cervix during Pregnancy

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