Molecular-Weight-Dependent Toxic Effects of Chitosans on the Human Keratinocyte Cell Line HaCaT

Wiegand, Cornelia; Winter, Dominic; Hipler, Uta‐Christina

doi:10.1159/000276996

Cited by 72 publications

(39 citation statements)

References 63 publications

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“…They actively migrated and uniformly occupied the deep layers of the chitosan scaffolds. It has been previously shown that pure chitosan with a molecular weight of 120 kDa induces apoptosis of HaCaT keratinocytes via activation of caspase-dependent signaling pathways [33]. Our results for N-TERT keratinocytes (much more "normal" than HaCaT cells) and chitosan with a molecular weight of 270 kDa contra- dict these data.…”

Section: Molecular Weight Of Chitosan and The Level Of Its Deacetylatsupporting

confidence: 77%

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

et al. 2015

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We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.

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Section: Molecular Weight Of Chitosan and The Level Of Its Deacetylatsupporting

confidence: 77%

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

et al. 2015

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“…The results obtained indicate that LCH sponges exhibit bio-safety at lower concentration (25%) during short time (1 day). The sponges prepared in our experiment are even less toxic than chitosan 1130 which has high molecular weight [23]. However, highly concentrated extraction shows a serious toxic effect on both HSF and HaCaT cells.…”

Section: Water Absorption Behaviormentioning

confidence: 69%

Study on Chitosan-Lactate Sponges with Oriented Pores as Potential Wound Dressing

Lai¹,

Chen²,

Zhang³

2013

MSA

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The motive of this work was to provide an inexpensive potential wound dressing using chitosan lactate (LCH) which was synthesized by the grafting lactic acid onto the amino groups in chitosan (CH) without a catalyst. The XRD and 13 C NMR results demonstrated that the grafting by lactic acid took place at C2 site in CH, leading to the destruction of the regularity of the packing in the original CH chains and formation of the amorphous CH salts. The unique device was developed in our experiments which could yield an approximately vertical thermal gradient, forming the uniformly vertical pores in LCH sponges. TEM images revealed that both TBA and LCH concentration affected the micro-structure of the sponges, although they worked via different mechanisms. In the water suction experiments, the capillary coefficient Ks was introduced to evaluate the structure-function relationship. The positive or negative influence of LCH, TBA and porosity on Ks clearly stood out when their relationships were plotted graphically. The in vitro biocompatibility of LCH sponges was evaluated. The results obtained indicated that LCH sponges exhibited bio-safety at lower concentration (25%) during short time (1 day). However, highly concentrated extraction showed a serious toxic effect on both HSF and HaCaT cells. The release kinetics for hydrophilic and hydrophobic drugs with different formulation sponges was determined in in vitro release experiments. The contribution of the drug diffusion, matrix erosion and microstructure of porous materials must be taken into account on the release mechanism. The method and the structure described in present paper provided a starting point for the design and fabrication of a family of chitosan derivatives based porous materials with potentially broad applicability

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“…Alternatively, chitosan might cause membrane disruption through electrostatic interaction, leading to an increase of the inflammatory cytokines IL-6 and IL-8. These cytokines have been shown to have mitogenic effects on normal fibroblasts and keratinocytes (16). Cancer cells might lose their ability to respond to the membrane-damaging effects of chitosan, such as the release of such inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Chitosan exerts anticancer activity through induction of apoptosis and cell cycle arrest in oral cancer cells

et al. 2014

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Molecular-Weight-Dependent Toxic Effects of Chitosans on the Human Keratinocyte Cell Line HaCaT

Cited by 72 publications

References 63 publications

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

Study on Chitosan-Lactate Sponges with Oriented Pores as Potential Wound Dressing

Chitosan exerts anticancer activity through induction of apoptosis and cell cycle arrest in oral cancer cells

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