Molecular Weight Dependence of the Antitumor Activity of Schizophyllan

Kojima, Takemasa; Tabata, Kengo; Itoh, Wataru; Yanaki, Toshio

doi:10.1080/00021369.1986.10867365

Cited by 26 publications

(30 citation statements)

References 4 publications

(4 reference statements)

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“…The total free‐energy change and the equilibrium constant for the overall transition from random coil to helix can be determined as function of chain length and interaction parameters 36. For schizophyllan a M of (25–40) × 10 3 g/mol has been identified as a range of minimum molecular mass required in order for the triplexes to remain stable 37. Below this M , the strands exist as single strands in aqueous solution.…”

Section: Properties and Structure Of Denatured And Renatured (13)‐β‐mentioning

confidence: 99%

Higher order structure of (1,3)‐β‐D‐glucans and its influence on their biological activities and complexation abilities

2008

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(1,3)‐β‐D‐Glucans form a group of biologically active biopolymers that exist in different structural organizations depending on the environmental conditions. The biological effect of (1,3)‐β‐D‐glucans is a core issue stimulating large research efforts of the molecular properties and their consequences for action as biological response modifiers. The fascination for these molecules increased further following the finding of their ability to form complexes of defined geometry with a number of structures, ranging from linear architectures as polymers or carbon nanotubes, to globular structures as gold particles or dye molecules. The fascinating information concerning the relationship between sample treatment history and molecular organization has not yet reached out to all the contributors within the field, resulting in unnecessary apparent inconsistencies in the literature. In addition to environmental conditions, the sample history is known to influence on the precise structural organization of these molecules. The present knowledge related to the structure of native as well as denatured, renatured and annealed (1,3)‐β‐D‐glucans is reviewed. The influence of their structural organization on the biological activity and complexation abilities is discussed, and some factors hindering progress in the understanding of their biological effects or complexation abilities are pointed out. © 2008 Wiley Periodicals, Inc. Biopolymers 89: 310–321, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley. com

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Section: Properties and Structure Of Denatured And Renatured (13)‐β‐mentioning

confidence: 99%

Higher order structure of (1,3)‐β‐D‐glucans and its influence on their biological activities and complexation abilities

2008

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“…3) backbone and the b(1 ! 6) branch of polysaccharides are probably responsible for their anti-tumor activity [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis

Shu

Lin

2006

Process Biochemistry

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“…It has been reported that the stiff glucans of triplexes including schizophyllan 43 and lentinan 44,45 have significant anti-tumor activities, which are strongly dependent on molecular weight and chain conformation. AF1 is a recently reported novel polysaccharide with a stiff chain conformation.…”

Section: Molecular Weights and Chain Conformation Of Af1 And Its Fracmentioning

confidence: 99%

Anti-hepatoma activity of the stiff branched β-d-glucan and effects of molecular weight

Ping

Liu

et al. 2016

J. Mater. Chem. B

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The water soluble b-(1 -3)-D-glucan with short branches (AF1) isolated from Auricularia auricula-judae was successfully fractionated by ultrasonication into three fractions with different weight-average molecular weights (M w s). The results of static and dynamic laser light scattering, viscometry and atomic force microscopy confirmed that the AF1 samples adopted a stiff chain conformation in water, and the coexistence of individuals and aggregates occurred gradually with increasing concentration. The AF1 sample with the highest M w easily self-entangled, and exhibited a strong shear rate-dependence of viscosity in water. The glucans displayed anti-hepatoma activity and significantly inhibited H22 tumour growth without cytotoxicity towards normal tissues. They displayed both molecular weight-and dosage-dependencies of anti-tumour activity, and the sample with an M w of 7.7 Â 10 5 at the dosage of 5 mg kg À1 exhibited the highest inhibition ratio of B77% against H22 tumour, even significantly higher than the positive control of cytoxan. The immunohistochemical and western blot analyses revealed that the AF1 glucans triggered cell apoptosis, indicated by the activation of caspase 3/9 and down-regulated tumour angiogenesis factors of VEGF and CD31. The underlying antitumor mechanism was suggested to induce tumour cell apoptosis and to inhibit angiogenesis in tumour tissues via enhancement of the immune-response. Taken together, the AF1 b-glucan was a potent natural drug candidate with high anti-cancer activities and less cytotoxicity, and the AF1 sample with a moderate molecular weight existed in aqueous solution as a more extended chain conformation, which plays an important role in activating immune responses.

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Molecular Weight Dependence of the Antitumor Activity of Schizophyllan

Cited by 26 publications

References 4 publications

Higher order structure of (1,3)‐β‐D‐glucans and its influence on their biological activities and complexation abilities

Higher order structure of (1,3)‐β‐D‐glucans and its influence on their biological activities and complexation abilities

Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis

Anti-hepatoma activity of the stiff branched β-d-glucan and effects of molecular weight

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