Molecular weight and structure characterization of sodium hyaluronate and its gamma radiation degradation products by flow field-flow fractionation and on-line multiangle light scattering

Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis

“…During exposure to irradiation, HA undergoes chemical degradation and depolymerization (62). Irradiation induces HA chain fragmentation, and dose-dependently affects the three-dimensional polymeric structure of HA (63,64). Thus, irradiation induces a synchronous disruption and degradation of HA, but does not interfere with HA synthesis (65)(66)(67).…”

Flow field-flow fractionation/multiangle light scattering of sodium hyaluronate from various degradation processes

“…The FlFFF channel used was the frit inlet asymmetrical FlFFF (FI-AFlFFF), which was the same as used in an earlier study [15]. The FI-AFlFFF channel dimensions were 27.2 cm × 0.0170 cm (length × thickness) with an initial breadth of 2.0 cm and a final breadth of 1.0 cm (trapezoidal), and a geometrical volume of 0.70 cm 3 .…”

“…Alkaline degradation of polysaccharides is known to occur with a cleavage of glycosidic linkages at the reducing terminus, and a recent report on alkaline degradation of molasses has been shown to produce de-icers [14]. In our earlier work, irradiation of HA using gamma rays was shown to produce a significant decrease in MW with a narrow MWD [15]. In optimizing the degradation process for the industrial use of HA, accurate characterization of MW and MWD as well as conformational information is necessary.…”

“…The first approach to resolve HA by FlFFF was tried by Wahlund and Litzen [27] to show the possibility of size fractionation with an asymmetrical FlFFF channel and, later FlFFF/MALS of HA was attempted by Takahashi et al [8]. Recently, Moon and coworkers [28] investigated the efficiency of field-programmed separation in Frit inlet asymmetrical FlFFF (FI-AFlFFF) for HA analysis and applied the method to study the effect of sample dissolution temperature and gamma radiation degradation on the size and conformation of NaHA [15,29,30].…”

Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: a review

“…A series of papers have been published in which AsFlFFF–MALS was applied to study the degradation of ultrahigh molecular weight hyaluronate under the influence of thermal treatment [29, 30], γ irradiation [31], and ultrasonic, enzymatic or alkaline treatment [32]. Degradation products with molecular masses around 50 kDa could be detected next to the original compounds with molecular masses up to 100 MDa.…”