Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions

Díaz‐Barrera, Alvaro; Sanchez-Rosales, Francisco; Padilla-Córdova, Claudio; Andler, Rodrigo; Peña, Carlos

doi:10.1007/s00449-021-02532-8

Cited by 8 publications

(9 citation statements)

References 42 publications

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“…In this line, recently Díaz-Barrera et al ( 2021 ) reported that, under oxygen limitation conditions (5.0 ± 0.9 mmol L −1 h −1 ) and no-nitrogen fixation, similar to those carried out in our study, A. vinelandii ATCC 9046 channeled the carbon source mainly to the production of biomass and intracellular polymers like PHB.…”

Section: Discussionsupporting

confidence: 89%

“…4 ). Other authors have reported that when OTR max was reduced, the μ value also decreased, although without changes in the final biomass concentration (Peña et al 2007 , 2011 ; Díaz-Barrera et al 2021 ). In our study, the growth of strain ATCC 12837 in BS medium under limited oxygenation conditions did not significantly affect growth, and also presented better cell viability, protein content, and lower qO 2 .…”

Section: Discussionmentioning

confidence: 92%

“…RQ was estimated from the quotient between the molar ratio of cumulative CO 2 production to cumulative O 2 utilization (Anderlei et al 2004 ). The specific oxygen uptake rate (qO 2 ) was obtained from the quotient between the OTR max value and the total protein content as previously described by Díaz-Barrera et al ( 2011 , 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

et al. 2021

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This study aimed to evaluate the growth, respiratory activity, and biodegradation of chlorpyrifos in cultures of Azotobacter vinelandii ATCC 12837. A strategy based on the modification of culture media and aeration conditions was carried out to increase the cell concentration of A. vinelandii, in order to favor and determine its tolerance to chlorpyrifos and its degradation ability. The culture in shaken flasks, using sucrose as a carbon source, significantly improved the growth compared to media with mannitol. When the strain was cultivated under oxygen-limited (5.5, 11.25 mmol L−1 h−1) and no-oxygen-limited conditions (22 mmol L−1 h−1), the growth parameters were not affected. In cultures in a liquid medium with chlorpyrifos, the bacteria tolerated a high pesticide concentration (500 ppm) and the growth parameters were improved even under conditions with a reduced carbon source (sucrose 2 g L−1). The strain degraded 99.6% of chlorpyrifos at 60 h of cultivation, in co-metabolism with sucrose; notably, A. vinelandii ATCC 12837 reduced by 50% the initial pesticide concentration in only 6 h (DT50). Graphical Abstract

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 92%

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Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

et al. 2021

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“…4). Other authors have reported that when OTR max was reduced, the µ value also decreased, although without changes in the nal biomass concentration (Peña et al 2007;Peña et al 2011;Díaz-Barrera et al 2021). In our study, the growth of strain ATCC 12837 in BS medium under limited oxygenation conditions did not signi cantly affect growth, and also presented better cell viability, protein content, and lower qO 2 .…”

Section: Growth and Respiratory Activity Of A Vinelandii Under Oxygen And Non-oxygen-limited Conditionsmentioning

confidence: 88%

Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

Conde-Avila

Peña

Pérez‐Armendáriz

et al. 2021

Preprint

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This study aimed to evaluate the growth, respiratory activity, and biodegradation of chlorpyrifos in cultures of Azotobacter vinelandii ATCC 12837. A strategy based on the modification of culture media and aeration conditions was carried out to increase the cell concentration of A. vinelandii , in order to favor and determine its tolerance to chlorpyrifos and its degradation ability. The culture in shaken flasks, using sucrose as a carbon source, significantly improved the growth compared to media with mannitol. When the strain was cultivated under oxygen-limited (5.5, 11.25 mmol L- 1 h- 1 ) and no-oxygen-limited conditions (22 mmol L -1 h -1 ), the growth parameters were not affected. In cultures in a liquid medium with chlorpyrifos, the bacteria tolerated a high pesticide concentration (500 ppm) and the growth parameters were improved even under conditions with a reduced carbon source (sucrose 2 g L -1 ). The strain degraded 99.6 % of chlorpyrifos at 60 h of cultivation, in co-metabolism with sucrose; notably, A. vinelandii ATCC 12837 reduced by 50% the initial pesticide concentration in only 6 h (DT 50 ).

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“…Some mucoid strains of P. aeruginosa biosynthesize great amounts of alginate containing mainly M-and MG-blocks (Table 1), often O-acetyl-esterified, which leads to an increasing interaction of the polymer with water molecules, polymer extension, and water capacity, thus allowing the development of a thickly natured and highly structured biofilm matrix [34][35][36]. Azotobacter spp., on the other hand, produce alginates that are closely associated with the cells and able to capture divalent ions such as Ca 2+ that form a hard and brittle gel due to the high content of L-guluronate residues (G-blocks) [37,38], allowing the formation of desiccation-resistant cysts under adverse environmental conditions [39,40].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Alginate Biosynthesis and Metabolism

Serrato¹

2024

Biochemistry

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Alginate is a linear anionic heteropolysaccharide with a chemical structure consisting of 1,4-linked subunits of β-D-mannuronic acid (M) and its C-5 epimer α-L-guluronic acid (G). It is well known that the monomer composition and molecular weight of alginates affect their properties and influence their use in the food and pharmaceutical industries. Alginate is usually extracted from seaweed for commercial purposes, but can also be produced by bacteria as exopolysaccharide (EPS). Pseudomonas spp. and Azotobacter vinelandii are well-known alginate-producing microorganisms. Their biochemical machinery for alginate biosynthesis is influenced by changing culture conditions and manipulating genes/proteins, making it relatively easy to obtain customized EPS with different molecular weights, M/G compositions, and thus physicochemical properties. Although these two genera have very similar biosynthetic pathways and molecular mechanisms for alginate production, with most of the genes involved being virtually identical, their regulation has been shown to be somewhat different. In this chapter, we present the main steps of alginate biosynthesis in bacteria, including precursor synthesis, polymerization, periplasmic modifications, transport/secretion, and post-secretion modification.

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Molecular weight and guluronic/mannuronic ratio of alginate produced by Azotobacter vinelandii at two bioreactor scales under diazotrophic conditions

Cited by 8 publications

References 42 publications

Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

Bacterial Alginate Biosynthesis and Metabolism

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