Molecular variation in plant cell populations evolving in vitro in different physiological contexts

Bogani, Patrizia; Simoni, Alessandra Traldi; Lió, Píetro; Germinario, Angela; Buiatti, M.

doi:10.1139/gen-44-4-549

“…The fact that we obtained four similar but not identical amplification products implies the validity of the method, since amplification products from any contaminants would be identical in sequence. The observed polymorphism was to be expected for a non-coding intervening sequence and it seemed more intense in polypurine and polypyrimidine stretches, in line with previous studies on hypervariable regions [2]. The variability of non-coding chloroplast DNA regions for phylogenetic studies and population genetic analysis is well documented [16].…”

Section: Discussionsupporting

confidence: 85%

Ultrastructure and DNA sequence analysis of single Concentricystis cells from Alta Val Tiberina Holocene sediment

Milanesi

¹

,

Vignani

²

,

Ciampolini

³

et al. 2006

Journal of Archaeological Science

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“…One approach is to examine the genome of regenerants with the use of molecular markers [18]. Hence, it is necessary to apply new approaches to studying the somaclonal variation in plants.…”

Section: Resultsmentioning

confidence: 99%

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

Kuznetsova

¹

,

Ash

²

,

Hartina

³

et al. 2005

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Long-term pea callus cultures of different genotypes (mutants R-9 and W-1 and cultivar Viola) were used to regenerate plants (generation R 0 ). The regenerants displayed changes both in qualitative and in quantitative traits. The most dramatic morphological alterations and complete sterility were observed in regenerants of the cultivar Viola. To estimate the genetic differences, regenerants were compared with the original lines with the use of RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) analyses. The extent of divergence varied among regenerants and depended mostly on the original genotype. The genetic difference from the original line was no more than 1% in W-1 regenerants, 0.7-5.3% in R-9 regenerants, and 10-15% in sterile regenerants of the cultivar Viola. The genetic variation of plants regenerated from a callus culture maintained for ten years did not exceed that of plants obtained from a culture maintained for two years. PLANT GENETICS

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“…Hence, it is necessary to apply new approaches to studying the somaclonal variation in plants. One approach is to examine the genome of regenerants with the use of molecular markers [18].…”

Section: Resultsmentioning

confidence: 99%

“…Products amplified with primer QR5 from DNAs of R-9 regenerants6,8,18,19, 45, 48, 49, and 56 and the original R-9 mutant (K + ). M, molecular weight marker (100 bp, SibEnzyme).…”

mentioning

confidence: 99%

RAPD and ISSR analyses of regenerated pea Pisum sativum l. plants

Кузнецова¹,

Ash

²

,

Hartina³

et al. 2005

View full text Add to dashboard Cite

Long-term pea callus cultures of different genotypes (mutants R-9 and W-1 and cultivar Viola) were used to regenerate plants (generation R 0 ). The regenerants displayed changes both in qualitative and in quantitative traits. The most dramatic morphological alterations and complete sterility were observed in regenerants of the cultivar Viola. To estimate the genetic differences, regenerants were compared with the original lines with the use of RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeat) analyses. The extent of divergence varied among regenerants and depended mostly on the original genotype. The genetic difference from the original line was no more than 1% in W-1 regenerants, 0.7-5.3% in R-9 regenerants, and 10-15% in sterile regenerants of the cultivar Viola. The genetic variation of plants regenerated from a callus culture maintained for ten years did not exceed that of plants obtained from a culture maintained for two years. PLANT GENETICS

show abstract

Molecular variation in plant cell populations evolving in vitro in different physiological contexts

Cited by 4 publications

References 0 publications

Ultrastructure and DNA sequence analysis of single Concentricystis cells from Alta Val Tiberina Holocene sediment

Ultrastructure and DNA sequence analysis of single Concentricystis cells from Alta Val Tiberina Holocene sediment

RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants

RAPD and ISSR analyses of regenerated pea Pisum sativum l. plants

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