Molecular variation in a novel polymorphic antigen associated with Plasmodium falciparum merozoites

McColl, Damian J.; Silva, Anabel; Foley, Michael; Kun, Jürgen F. J.; Favaloro, Jenny M.; Thompson, J. K.; Marshall, Vikki M.; Coppel, Ross L.; Kemp, David J.; Anders, Robin F.

doi:10.1016/0166-6851(94)00149-9

Cited by 105 publications

(81 citation statements)

References 36 publications

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“…6 § Strain D10. 4 ¶ Strain F32. 5 first and the fourth time points, there was a clearly dominant MSP2 allele but this was less marked for AMA1.…”

Section: Resultsmentioning

confidence: 99%

“…Whereas new allelic forms of MSP1, MSP2, and AMA1 were identified in this study, MSP3 alleles were identical to those previously described. 4,25 There is only a single complete GLURP sequence in Genbank, 5 and these three sequences differ in R2 repeat number. AMA1 is the most sensitive isolate marker, with seven different alleles present.…”

Section: Discussionmentioning

confidence: 99%

“…The proteins include integral membrane proteins such as apical membrane antigen 1 (AMA1) 1 and merozoite surface proteins (MSP) 1 2 and 2 3 . Others such as the MSP3 4 and the glutamate-rich protein (GLURP) 5 are peripheral membrane proteins that associate with the merozoite surface by protein-protein interactions.…”

Section: Introductionmentioning

confidence: 99%

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Alterations in Plasmodium falciparum genotypes during sequential infections suggest the presence of strain specific immunity.

Eisen

Saul²,

Fryauff³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. Many of the asexual stage Plasmodium falciparum proteins that are the targets of host protective responses are markedly polymorphic. The full repertoire of diversity is not defined for any antigen. Most studies have focused on the genes encoding merozoite surface proteins 1 and 2 (MSP1, MSP2). We explored the extent of diversity of some of the less studied merozoite surface antigens and analyzed the degree of complexity of malaria field isolates by deriving nucleotide sequences of several antigens. We have determined the genotype of apical membrane antigen 1 (AMA1) in a group of 30 field samples, collected over 29 months, from individuals living in an area of intense malaria transmission in Irian Jaya, identifying 14 different alleles. AMA1 genotyping was combined with previously determined MSP2 typing. AMA1 had the greatest power in distinguishing between isolates but methodological problems, especially when mixed infections are present, suggest it is not an ideal typing target. MSP1, MSP3, and glutamate-rich protein genotypes were also determined from a smaller group of samples, and all results were combined to derive an extended antigenic haplotype. Within this subset of 10 patients, nine different genotypes could be discerned; however, five patients were all infected with the same strain. This strain was present in individuals from two separate villages and was still present 12 months later. This strain was predominant at the first time point but had disappeared at the fourth time point. This significant change in malaria genotypes could be due to strain-specific immunity developing in this population.

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“…6 § Strain D10. 4 ¶ Strain F32. 5 first and the fourth time points, there was a clearly dominant MSP2 allele but this was less marked for AMA1.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Alterations in Plasmodium falciparum genotypes during sequential infections suggest the presence of strain specific immunity.

Eisen

Saul²,

Fryauff³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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“…In brief, for difference spectra, 10 M hemin from a stock of 1 mM was added simultaneously in both test and reference cuvettes. The test cuvette contained 10 M protein (MSP3F, MSP3N, MSP3Ca, MSP3Cb, MSP3Cc, or polyhistidine-tagged MSP 19 (control)) in 20 mM Tris, 150 mM NaCl, pH 8.0, and only buffer was added in reference cuvette.…”

Section: Expression and Purification Of Msp3 Recombinant Proteins-mentioning

confidence: 99%

“…high histidine/cysteine content, etc., that would explain its heme binding properties (5). However, MSP3 contains three domains of alanine heptad repeats, a glutamic acid-rich region and a C-terminal leucine zipper-like motif (19). The presence of a specific 40-residue sequence in the leucine zipper region has been implicated in the formation of dimers and tetramers of MSP3 (16,17).…”

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confidence: 99%

Plasmodium falciparum Merozoite Surface Protein 3

Imam

Singh

Kaushik

et al. 2014

Journal of Biological Chemistry

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Background: Plasmodium falciparum merozoite surface protein 3 (MSP3) oligomerizes and binds heme. Results: MSP3 forms amyloid-like structures that bind ϳ35 heme molecules, and these filamentous structures are also present on the surface of merozoites. Conclusion: Amyloid formation by MSP3 is related to heme binding. Significance: The presence of MSP3 fibrils on merozoite surface and significant heme binding suggest its functional role during erythrocytic stages of P. falciparum.

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