Molecular variability of the root-lesion nematode, Pratylenchus loosi (Nematoda: Pratylenchidae), from tea in Iran

Mirghasemi, Seyedeh Negin; Fanelli, Elena; Troccoli, Alberto; Jamali, Salar; Sohani, Mohammad Mehdi; Luca, Francesca De

doi:10.1007/s10658-019-01792-1

Cited by 5 publications

(7 citation statements)

References 33 publications

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“…One advantage of Hsp90 over rDNA genes is that it was found to be single copy in Caenorhabditis elegans ( Birnby et al ., 2000 ), Heterodera glycines , and Meloidogyne javanica ( Skantar and Carta, 2004 ). However, intraspecific variation in Hsp90 was found in Pratylenchus species, with different variants suggested to be important for nematode adaptation to different environmental stresses and host plants ( Fanelli et al ., 2018 ; Mirghasemi et al ., 2019 ; Troccoli et al ., 2021 ). The Hsp90 gene may also be multi-copy in H. carotae ( Madani et al ., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Genome Characterization and Development of Real-Time PCR Assays for Ditylenchus dipsaci and D. weischeri

Пономарева

Badiss

Sultana

et al. 2022

Journal of Nematology

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The stem and bulb nematode Ditylenchus dipsaci is a destructive nematode pest on many crops and is internationally quarantined in many countries, whereas Ditylenchus weischeri, only known to infect a weed plant (Cirsium arvense), is an unregulated nematode species with no known economic importance. In this study, we used comparative genomics to identify multiple gene regions and developed novel real-time PCR assays for the detection of D. dipsaci and D. weischeri. We sequenced the genomes of two mixed-stage nematode populations of D. dipsaci and two mixed-stage nematode populations of D. weischeri. The assembled genomes of D. dipsaci were 228.2 Mb and 239.5 Mb, and the genomes of D. weischeri were 177.0 Mb and 196.3 Mb. Depending on the species, 21,403–27,365 gene models were predicted. Using orthologous group analysis, single-copy and species-specific genes were identified. Primers and probes were designed targeting two species-specific genes in each species. The assays detected as low as 12 pg of DNA from the target species, or as few as five nematodes, with a Cq of 31 cycles or less. Our study provides genome data for two additional D. dipsaci isolates and two D. weischeri isolates, and four new and validated molecular assays to be used for rapid detection and identification of the two species.

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Section: Discussionmentioning

confidence: 99%

Genome Characterization and Development of Real-Time PCR Assays for Ditylenchus dipsaci and D. weischeri

Пономарева

Badiss

Sultana

et al. 2022

Journal of Nematology

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“…(2020) reported a low intraspecific variability of 0 bp to 8 bp (0%–1.93%) among populations of P. hexincisus from China, Italy, and the United States and a high intraspecific variability of 54 bp to 102 bp (19.1%–24.5%) among populations from the same countries. Mirghasemi et al . (2019) reported a low intraspecific variability of 0.0% to 0.96% (0–4 bp) between populations of P. loosi from Iran, China, and Japan.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the results of these investigations, the intraspecific variability recorded for P. araucensis in the present study can be considered low. However, the nucleotide differences between populations of P. araucensis from Valle del Cauca and those from Caldas and Quindío may be related to contrasting climatic differences between regions, specifically rainfall ( Castillo and Vovlas, 2007 ; Mirghasemi et al ., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Pratylenchus araucensis (Rhabditida: Pratylenchidae) a Widely Distributed Nematode in Musa spp. From Colombia

Arboleda-Riascos

Riascos-Ortiz

Agudelo

et al. 2022

Journal of Nematology

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Pratylenchus is one of the most limiting nematodes of Musaceae production in the world. Knowledge of the nematode species is one of the requirements for its management in the field. This study aimed to identify up to the species level Pratylenchus populations associated with plantain and banana crops in the states of Caldas, Quindío, and Valle del Cauca in Colombia. In these regions, Pratylenchus has been reported to affect these crops in the past, but with records of the nematode only up to the genus level. For this purpose, five populations of Pratylenchus extracted from samples composed of roots and rhizospheric soil, four from plantain and one from banana, were identified through morphological, morphometric, and molecular analysis (sequencing of the D2–D3 of rRNA and cytochrome oxidase subunit I of mtDNA). All populations were identified as P. araucensis, a species reported previously in eastern Colombia, and one that the present study found in the center and southwest of the country, indicating that this species of nematode is widely distributed in the Musaceae-producing areas of Colombia. The present study reports the first COI mtDNA sequences for this species of nematode.

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“…For all stages, the optimum temperatures are 18–24°C (Gnanapragasham & Mohotti, 2018 ). Highest population densities may occur in spring and autumn (Seraji et al., 2006 ). Nematodes move only short distances over a year.…”

Section: Pest Categorisationmentioning

confidence: 99%

Pest categorisation of Pratylenchus loosi

Bragard,

Baptista,

Chatzivassiliou

et al. 2024

EFS2

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Following the EFSA commodity risk assessment of Malus domestica plants imported from Türkiye into the EU, the EFSA Panel on Plant Health performed a pest categorisation of Pratylenchus loosi (Nematoda: Pratylenchidae) for the EU. Pratylenchus loosi belongs to the order Rhabditida, subfamily Pratylenchidae. This nematode is not known to be present in the EU. The species is not included in the EU Commission Implementing Regulation 2019/2072. The pest occurs primarily in tropical, subtropical and warm temperate areas. It is widely distributed in Asian countries, with tea plants (Camellia sinensis) as the main host. The pest was reported from more than 60 plant species, but reports from hosts other than C. sinensis, e.g. citrus (Citrus spp.) and banana (Musa spp.), are associated with high uncertainty due to doubtful pest identification. Morphological and molecular methods are available for the identification of the pest. Pathways of entry are host plants for planting except seeds, as well as soil attached to plants for planting, machinery or footwear. Soil import to the EU is prohibited from third countries. The climatic preferences of P. loosi are compatible with the microclimatic conditions occurring in the areas of the EU where tea is grown outside. The impact of the nematode is primarily known for Asian countries, where it is a devastating pathogen on tea plants, but there is a key uncertainty on impacts on hosts other than tea. Considering the strong pathogenicity of the pest, its establishment in tea producing areas would have negative consequences for tea producers. Therefore, the Panel concludes that P. loosi satisfies all the criteria that are within the remit of EFSA to assess for it to be regarded as a potential Union quarantine pest.

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Molecular variability of the root-lesion nematode, Pratylenchus loosi (Nematoda: Pratylenchidae), from tea in Iran

Cited by 5 publications

References 33 publications

Genome Characterization and Development of Real-Time PCR Assays for Ditylenchus dipsaci and D. weischeri

Genome Characterization and Development of Real-Time PCR Assays for Ditylenchus dipsaci and D. weischeri

Pratylenchus araucensis (Rhabditida: Pratylenchidae) a Widely Distributed Nematode in Musa spp. From Colombia

Pest categorisation of Pratylenchus loosi

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