Molecular validation of anthropophilic Phlebotominae sandflies (Diptera: Psychodidae) in Central Panama

Dutari, Larissa C.; Loaiza, José R.

doi:10.1590/0074-02760190034

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Here we summarize some of the results in light of their findings and to contrast with our proposed phylogeny. Dutari & Loaiza [ 15 ] focused primarily on sandflies from Panama recovering Pi. ovallesi and Mi.…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of Psychodopygina (Diptera, Psychodidae) supporting morphological systematics of this group of vectors of New World tegumentary leishmaniasis

et al. 2023

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New World sandflies are vectors of leishmaniasis, bartonellosis, and some arboviruses. A classification based on 88 morphological characters was proposed 27 years ago when the New World phlebotomines were organized into two tribes Hertigiini and Phlebotomini. The latter was structured into four subtribes (Brumptomyiina, Sergentomyiina, Lutzomyiina, and Psychodopygina) and 20 genera. The subtribe Psychodopygina, including most of the American vectors of tegumentary Leishmania comprises seven genera from which no molecular work has been produced to support this classification. Here, we carried out a molecular phylogeny based on combined sequences (1,334 bp) of two genes: partial 28S rDNA and mtDNA cytochrome b from 47 taxa belonging to the Psychodopygina. The Bayesian phylogenetic reconstruction agreed with the classification based on morphological characters, supporting the monophyly of the genera Psychodopygus and Psathyromyia, whereas Nyssomyia and Trichophoromyia seemed to be paraphyletic. The paraphylies of the two latter groups were exclusively caused by the doubtful position of the species Ny. richardwardi. Our molecular analysis provides additional support to adopt the morphologic classification of Psychodopygina.

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Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of Psychodopygina (Diptera, Psychodidae) supporting morphological systematics of this group of vectors of New World tegumentary leishmaniasis

et al. 2023

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“…In our analysis (ML and BI), we now included this molecular information and confirm that in Central and South America the molecular data only correspond to the monophyletic species Bi . olmeca bicolor [ 48 , 52 – 54 ], whereas the sequences of the COI and cytb genes from Mexico available in GenBank and the sequences generated in this study ( COI / cytb / 18S rDNA), separate Bi . olmeca into another monophyletic group [ 15 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic relations among Mexican phlebotomine sand flies (Diptera: Psychodidae) and their divergence time estimation

Lozano-Sardaneta,

Díaz-Cruz,

Viveros-Santos

et al. 2023

PLoS ONE

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Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) have biological relevance as vectors of several pathogens. To ensure periodic entomological monitoring it is necessary to have efficient and accurate tools for an adequate taxonomic identification. There are only few studies on phylogenetic analyses of phlebotomine sand flies from Neotropics, based mostly on morphological and/or molecular data, which makes the delimitation of intra- and interspecific variability of species challenging. Here we generated new molecular information on sand fly species distributed in endemic areas of leishmaniasis in Mexico, using mitochondrial and ribosomal genes, and incorporating morphological information available. Specifically, we established their phylogenetic relationships, and estimated their divergence time. Our study provides molecular information for 15 phlebotomine sand fly species from different areas of Mexico, contributing to the genetic inventory and phylogenetic relations among Neotropical species of the subfamily Phlebotominae. Mitochondrial genes proved to be suitable markers for the molecular identification of phlebotomine sand flies. However, the incorporation of additional nuclear gene information could increase the significance of phylogenetic inferences. We also provided evidence about a possible divergence time of phlebotomine sand fly species, supporting their presumable origin in the Cretaceous period.

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Molecular validation of anthropophilic Phlebotominae sandflies (Diptera: Psychodidae) in Central Panama

Cited by 2 publications

References 25 publications

Molecular phylogeny of Psychodopygina (Diptera, Psychodidae) supporting morphological systematics of this group of vectors of New World tegumentary leishmaniasis

Molecular phylogeny of Psychodopygina (Diptera, Psychodidae) supporting morphological systematics of this group of vectors of New World tegumentary leishmaniasis

Phylogenetic relations among Mexican phlebotomine sand flies (Diptera: Psychodidae) and their divergence time estimation

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