Molecular typing of Yersinia pseudotuberculosis by using an IS200-like element

Odaert, M.; Berche, Patrick; Simonet, Michel

doi:10.1128/jcm.34.9.2231-2235.1996

Cited by 23 publications

(13 citation statements)

References 36 publications

(36 reference statements)

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“…In a previous study, we showed that HincII-restricted DNA from Y. pseudotuberculosis strain IP2777 (5.8, 4.3, 2.9, 2.6, 2.3, 2.0, 1.8 and 1 kb) and Y. enterocolitica strain 8081 (3.1 and 2.3 kb) contained sequences homologous to IS1541 [2]. We assumed that each hybridizing band harbored the unrestricted IS sequence since there was no HincII restriction site within IS1541.…”

Section: Cloning Is1541-related Elements From Y Pseudotuberculosis Amentioning

confidence: 98%

“…Y. pseudotuberculosis strain IP2777 [2], Y. enterocolitica O:8 strain 8081 [2] and the cloning host strain DH5K of Escherichia coli were used in this study. Yersinia DNA was inserted into vector pUC19 (Appligene, Illkirch, France).…”

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

“…It contains a single open reading frame (ORF) which encodes a putative 152-amino acid protein. Sequences related to IS1541 were also detected by Southern blot analysis in the genomes of the two enteropathogenic Yersinia species Yersinia pseudotuberculosis and Yersinia enterocolitica [2]. By contrast, DNA from the non-pathogenic species Yersinia frederiksenii, Yersinia kristensenii, Yersinia intermedia, Yersinia bercovieri and Yersinia mollaretii did not hybridize with this probe [2].…”

Section: Introductionmentioning

confidence: 95%

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Characterization of IS1541-like elements in Yersinia enterocolitica and Yersinia pseudotuberculosis

Devalckenaere

1999

FEMS Microbiology Letters

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We characterized Yersinia enterocolitica and Yersinia pseudotuberculosis insertion sequences related to insertion sequence 1541, recently identified in Yersinia pestis. For each of the two species, two insertion sequence copies were cloned and sequenced. Genetic elements from Y. pseudotuberculosis were almost identical to insertion sequence 1541, whereas these from Y. enterocolitica were less related. Phylogenetic analysis of the putative transposases encoded by insertion sequences from the three pathogenic members of the genus Yersinia showed that they clustered with those encoded by Escherichia coli and Salmonella enterica elements belonging to the insertion sequence 200/insertion sequence 605 group. Insertion sequences originating from Y. pestis and Y. pseudotuberculosis constitute a monophyletic lineage distinct from that of Y. enterocolitica. ß

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Section: Cloning Is1541-related Elements From Y Pseudotuberculosis Amentioning

confidence: 98%

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Characterization of IS1541-like elements in Yersinia enterocolitica and Yersinia pseudotuberculosis

Devalckenaere

1999

FEMS Microbiology Letters

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“…With time, IS elements can shift locations, replicate into other genomic positions, and can be excised from the genome. Collectively, we refer to these changes in the number and positions of IS elements IS200-like element [33] as transposition events. Polymorphism among strains results from variation in the number of elements, or the copy number, and their positions in the genome of the pathogen.…”

Section: Introductionmentioning

confidence: 99%

Optimal estimation of transposition rates of insertion sequences for molecular epidemiology

Tanaka

Rosenberg

2001

Statistics in Medicine

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Outbreaks of infectious disease can be confirmed by identifying clusters of DNA fingerprints among bacterial isolates from infected individuals. This procedure makes assumptions about the underlying properties of the genetic marker used for fingerprinting. In particular, it requires that each fingerprint changes sufficiently slowly within an individual that isolates from separate individuals infected by the same strain will exhibit similar or identical fingerprints. We propose a model for the probability that an individual's fingerprint will change over a given period of time. We use this model together with published data in order to estimate the fingerprint change rate for IS6110 in human tuberculosis, obtaining a value of 0.0139 changes per copy per year. Although we focus on insertion sequences (IS), our method applies to other fingerprinting techniques such as pulsed-field gel electrophoresis (PFGE). We suggest sampling intervals that produce the least error in estimates of the fingerprint change rate, as well as sample sizes that achieve specified levels of error in the estimate.

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“…IS200 has been extensively used for the typing of Salmonella isolates of numerous serotypes by RFLP analysis (8,14,24), an approach that could be called IS200 typing (9). Similarly, molecular typing by RFLP analysis for IS1541 of isolates of Yersinia pseudotuberculosis has been described (29).…”

mentioning

confidence: 99%

Evaluation of IS200-PCR and Comparison with Other Molecular Markers To Trace Salmonella enterica subsp.enterica Serotype Typhimurium Bovine Isolates from Farm to Meat

Millemann

Gaubert

Remy

et al. 2000

J. Clin. Microbiol.

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A procedure that uses an original molecular marker (IS200-PCR) and that is based on the amplification of DNA with outward-facing primers complementary to each end of IS200 has been evaluated with a collection of 85 Salmonella enterica subsp. enterica serotype Typhimurium isolates. These strains were isolated from a group of 10 cows at different stages: during transportation between the farm and the slaughterhouse, on the slaughter line, from the environment, and from the final product (ground beef). The 85 isolates were characterized by their antibiotic resistance patterns and were compared by IS200-PCR and by use of four other genotypic markers. Those markers included restriction profiles for 16S and 23S rRNA (ribotypes) and amplification profiles obtained by different approaches: random amplified polymorphic DNA analysis, enterobacterial repetitive intergenic consensus PCR, and PCR ribotyping. The results of the IS200-PCR were in accordance with those of other molecular typing methods for this collection of isolates. Five different genotypes were found, which made it possible to refine the hypotheses on transmission obtained from phenotypic results. The genotyping results indicated the massive contamination of the whole group of animals and of the environment by one clonal strain originally recovered from one cow that excreted the strain. On the other hand, a few animals and their environment appeared to be simultaneously contaminated with genetically different strains.

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Molecular typing of Yersinia pseudotuberculosis by using an IS200-like element

Cited by 23 publications

References 36 publications

Characterization of IS1541-like elements in Yersinia enterocolitica and Yersinia pseudotuberculosis

Characterization of IS1541-like elements in Yersinia enterocolitica and Yersinia pseudotuberculosis

Optimal estimation of transposition rates of insertion sequences for molecular epidemiology

Evaluation of IS200-PCR and Comparison with Other Molecular Markers To Trace Salmonella enterica subsp.enterica Serotype Typhimurium Bovine Isolates from Farm to Meat

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