Molecular typing of Streptococcus suis isolates from Iberian pigs: A comparison with isolates from common intensively-reared commercial pig breeds

Rey, Verónica Sánchez del; Fernández‐Garayzábal, J. F.; Bárcena, Carmen; Briones, V.; Domı́nguez, Lucas; Gottschalk, Marcelo; Vela, A. I.

doi:10.1016/j.tvjl.2014.10.020

Cited by 5 publications

(5 citation statements)

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“…[ 54 ] in North America. This phenomenon has also been observed in other countries, such as Thailand [ 20 , 53 ], Spain [ 58 , 60 ], and Canada [ 15 , 52 ]. The great variability in the prevalence of S. suis serotype 2 isolated from pigs among the included studies, even in the same region, reflected the difference in study settings of the included studies and depended on several factors such as coinfection with porcine reproductive and respiratory syndrome virus at weaning, introduction of pigs into the nursery, sow parity, relative humidity, and temperature [ 7 , 70 , 71 ].…”

Section: Discussionsupporting

confidence: 62%

Prevalence of Streptococcus suis serotype 2 isolated from pigs: A systematic review and meta-analysis

Keonam,

Nam,

Saksangawong

et al. 2024

Vet World

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Background and Aim: Among Streptococcus suis serotypes, S. suis serotype 2 is the most significant serotype that causes serious diseases in pigs and humans worldwide. The present study aimed to estimate the global prevalence of S. suis serotype 2 isolated from pigs, determine its trend, and explore the factors associated with this serotype. Materials and Methods: We retrieved relevant published studies from PubMed, Scopus, and the Web of Science. The retrieved citations were screened for possible inclusion. Relevant data were then extracted from the included studies. The random-effects model was used for all meta-analyses. A subgroup meta-analysis was used to assess the heterogeneity of the prevalence for four characteristics (continents, sampling organs, reporting unit, and pig’s health status). A cumulative meta-analysis was performed to determine the cumulative prevalence over time. Meta-regression analysis was used to determine the trend of pooled prevalence of S. suis serotype 2 over time. Results: Of 600 articles retrieved, 36 studies comprising a total sample size of 6939 isolates or samples from 16 countries of four continents were included for meta-analysis. The pooled prevalence of S. suis serotype 2 isolated from pigs was 13.6% (95% confidence interval [CI], 10.7%–17.1%), with high heterogeneity among the included studies (Cochran’s Q, 431.6; p < 0.001; I2 = 91.9%; Table-1). No statistical significance was observed among subgroups of the four characteristics examined. However, the pooled prevalence of S. suis serotype 2 was as high as 16.0% (95% CI, 12.5%–20.3%; n = 16) in diseased pigs compared with 9.9% (95% CI, 5.6%–17.0%; n = 15) in healthy pigs. The pooled prevalence of S. suis serotype 2 isolated from pigs did not significantly decrease over time [regression coefficient = −0.020 (95% CI, 0.046–0.006, p = 0.139)]. The pooled prevalence of S. suis serotype 2 isolated from pigs fluctuated slightly between 13.2% and 17.8% from 2007 to 2023, although the pooled prevalence gradually decreased from 30.6% in 1987 to over 20% in 2003. Conclusion: The global prevalence of S. suis serotype 2 isolated from pigs was estimated to be 13.6% (approximately 10% in healthy pigs and around 16% in diseased pigs). S. suis serotype 2 isolated from pigs did not change significantly over time. These results indicate that S. suis serotype 2 remains a problem for the pig industry and poses a threat to human health. Keywords: meta-analysis, pigs, prevalence, serotype 2, Streptococcus suis.

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Section: Discussionsupporting

confidence: 62%

Prevalence of Streptococcus suis serotype 2 isolated from pigs: A systematic review and meta-analysis

Keonam,

Nam,

Saksangawong

et al. 2024

Vet World

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“…This serotype has been frequently isolated from diseased animals in intensively-reared commercial pig breeds, particularly in Spain, Germany, and the Netherlands [ 6 ]. Interestingly, it has also been isolated from Iberian pigs reared outdoors, wild boars, and even wild rabbits in Spain [ 8 – 10 ]. Epidemiological studies suggest that the prevalence of serotype 9 has also increased in China over recent years [ 11 ] and, to a much lesser extent, in North America (Canada) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Zheng¹,

Du²,

Qiu³

et al. 2018

Vet Res

101

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Streptococcus suis is one of the most important bacterial pathogens in the porcine industry and also a zoonotic agent. Serotype 9 is becoming one of the most prevalent serotypes within the S. suis population in certain European countries. In the present study, serotype 9 strains isolated from a country where infection due to this serotype is endemic (Spain), were compared to those recovered from Canada, where this serotype is rarely isolated from diseased pigs. For comparison purposes, strains from Brazil and the only strain isolated from a human case, in Thailand, were also incorporated. Firstly, sequence types (STs) were obtained followed by detection of putative virulence factors. Phylogenetic trees were constructed using the non-recombinant single nucleotide polymorphisms from core genomes of tested strains. Most Spanish strains were either ST123 or ST125, whereas Canadian strains were highly heterogeneous. However, the distribution of putative virulence factors was similar in both groups of strains. The fact that ST16 strains harbored more putative virulence genes and shared greater similarity with the genome of human serotype 2 strains suggests that they present a higher zoonotic and virulence potential than those from Canada and Spain. More than 80% of the strains included in this study carried genes associated with resistance to tetracycline, lincosamides and macrolides. Serotype 9 strains may be nearly 400 years old and have evolved in parallel into 2 lineages. The rapid population expansion of dominant lineage 1 occurred within the last 40 years probably due to the rapid development of the porcine industry.Electronic supplementary materialThe online version of this article (10.1186/s13567-017-0498-2) contains supplementary material, which is available to authorized users.

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“…However, the 73 non-typable isolates included 6 isolates that reacted with multiple serotypes: a first isolate with serotypes 4 and 9, a second isolate with serotypes 6, 12, 23, and 30, and a third isolate with serotypes 6, 23, and 30, while three more isolates reacted with serotypes 23 and 30. Isolates reacting with multiple serotypes are commonly isolated from tonsils [2]. Multiple serotypes may be the result of similar capsular polysaccharide composition among the different serotypes, which are probably due to recombination [13].…”

Section: Discussionmentioning

confidence: 99%

Serotypes of Streptococcus suis isolated from healthy pigs in Phayao Province, Thailand

Thongkamkoon¹,

Kiatyingangsulee

Gottschalk

2017

BMC Res Notes

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Background Streptococcus suis (S. suis) is an important swine and human pathogen. There are 33 serotypes that have been described. Zoonotic cases are very common the Northern part of Thailand, especially in Phayao Province. However, the prevalence of S. suis and, more particularly the different serotypes, in pigs in this region is poorly known and needed to be addressed.The context and purpose of the studyDistribution of S. suis serotypes varies depending on the geographical area. Knowledge of the serotype distribution is important for epidemiological studies. Consequently, 180 tonsil samples from slaughterhouse pigs in Phayao Province had been collected for surveillance, from which 196 S. suis isolates were recovered. Each isolate was subcultured and its serotype identified using multiplex PCR. Slide agglutination combined with precipitation tests were used following multiplex PCR to differentiate the isolates showing similar sizes of amplified products specific to either serotype 1 or 14 and 2 or 1/2. Non-typable isolates by multiplex PCR were serotyped by the coagglutination test.ResultsOf the 196 isolates, 123 (62.8%) were typable and 73 (37.2%) were non-typable. This study revealed the presence of serotypes 1, 1/2, 2, 3, 4, 5, 7, 9, 11, 12, 13, 14, 21, 22, 23, 24, 25, 29, and 30. Serotype 23 was the most prevalent (20/196, 10.2%), followed by serotype 9 (16/196, 8.2%), serotype 7 (16/196, 8.2%), and serotype 2 (11/196, 5.6%). The latter is the serotype responsible for most human cases.ConclusionAlmost all serotypes previously described are present in Northern Thailand. Therefore, this report provides useful data for future bacteriological studies.

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Molecular typing of Streptococcus suis isolates from Iberian pigs: A comparison with isolates from common intensively-reared commercial pig breeds

Cited by 5 publications

References 24 publications

Prevalence of Streptococcus suis serotype 2 isolated from pigs: A systematic review and meta-analysis

Prevalence of Streptococcus suis serotype 2 isolated from pigs: A systematic review and meta-analysis

Genomic comparisons of Streptococcus suis serotype 9 strains recovered from diseased pigs in Spain and Canada

Serotypes of Streptococcus suis isolated from healthy pigs in Phayao Province, Thailand

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