Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis

Kang, Xiangtao; Chen, Meng; Pan, Zhiming

doi:10.1007/978-1-0716-0791-6_17

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…The standard strain Salmonella Braenderup H9812 (ATCC ® BAA-664 ™ ) was chosen as the universal size standard. Staining gel after electrophoresis used ethidium bromide and sterile ultrapure water was used to destain (Kang et al, 2021).…”

Section: Pulsed-field Gel Electrophoresis (Pfge)mentioning

confidence: 99%

Colistin resistance and resistance determinants are mobile amongSalmonella entericaisolates from diseased and healthy pigs in Thailand

Prasertsee

Pascoe

Patchanee

2023

Preprint

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Salmonella is an important enteric pathogen that poses a threat to human and livestock animal health, with emerging multidrug resistance (MDR) a major public health issue globally. We investigated the prevalence of Salmonella in healthy and diseased pigs from Thai pig farms and determined their phenotypic and genotypic antimicrobial resistance profiles. A total of 150 fecal samples were collected from pigs housed in pens from four separate pig farms in southern Thailand and tested for the presence of Salmonella. Confirmed Salmonella isolates were tested for their susceptibility to 11 antimicrobials, and PCR used to detect known antimicrobial resistance genes (ARGs). Salmonella isolates were cultured from 69% (103/150) of all fecal samples, with higher prevalence in disease pigs (12/15; 80%), compared with healthy pigs (91/135; 67%). Serotype Rissen was the most frequently identified serotype among the Salmonella isolates. Resistance to ampicillin (AMP) (97%), sulfonamide-trimethoprim (SXT) (97%), and tetracycline (TET) (94%) were the most common phenotypes observed. The most common ARGs identified were blaTEM gene (99.%), tetA (87%), sul1 (77%), and dfrA1 (74%), and more than 95% of the Salmonella isolates tested were MDR - based on resistance to three or more antimicrobial classes. The most common antimicrobial resistance pattern exhibited was AMP-TET-SXT (76%), and resistance to colistin (via the mcr-1 gene) was observed in both healthy and diseased pigs. The clonal groups of PFGE analysis in serotype Typhimurium revealed the genetic relationship among Salmonella isolated from healthy and diseased pigs from different pig farms.

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Section: Pulsed-field Gel Electrophoresis (Pfge)mentioning

confidence: 99%

Colistin resistance and resistance determinants are mobile amongSalmonella entericaisolates from diseased and healthy pigs in Thailand

Prasertsee

Pascoe

Patchanee

2023

Preprint

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“…It plays a significant role epidemic control and tracking the sources of infection. Among many molecular methods, the PFGE method is currently known the "gold standard" of molecular typing methods for bacterial pathogens and nosocomial infections (8,9). In an epidemic case, rapid clustering can be performed with PFGE and epidemiologically related cases can be easily distinguished from sporadic cases by this method.…”

Section: Introductionmentioning

confidence: 99%

Molecular Typing with PFGE Method of Methicillin-resistant Staphylococcus aureus Isolates from in a University Hospital

SAVAS¹,

Parlak²

2021

HMJ

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Staphylococcus aureus is a pathogen that causes many infections in humans, and the emergence of methicillin-resistant (MRSA) isolates in the last fifty years poses a threat to society and hospitals. Preventing the spread of MRSA strains between and within hospitals can only be achieved with a correct typing. The PFGE method is considered to be the gold standard among molecular typing methods. Materials and MethodsIn this study, a clonal relationship between nine methicillin-resistant S. aureus isolates isolated from Van Yüzüncü Yıl University hospital was determined .Pfge analysis was done with the Bionumerics program. The similarity index was created at a tolerance value of 1% using the "Dice" coefficient. The clustering relationship between isolates was demonstrated using the UPGMA (unweighted pair group method of arithmatic averages) method. The principles set out by Tenover et al. were adopted for the evaluation of genotypic association between isolates ResultsAs a result of the analysis 4 independent genotypes were identified. It was determined that there were 2 related isolates in 3 genotypes. ConclusionIn our study, it was determined that there was not a dominant MRSA clone in different years in the hospital studied, since samples from different years were studied. However, it is thought that it would be beneficial to increase the precautions in order to prevent the distribution of infection in all hospitals and to control the infection in all areas.

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Molecular Typing of Salmonella by Pulsed-Field Gel Electrophoresis

Cited by 2 publications

References 16 publications

Colistin resistance and resistance determinants are mobile amongSalmonella entericaisolates from diseased and healthy pigs in Thailand

Colistin resistance and resistance determinants are mobile amongSalmonella entericaisolates from diseased and healthy pigs in Thailand

Molecular Typing with PFGE Method of Methicillin-resistant Staphylococcus aureus Isolates from in a University Hospital

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