Molecular typing of Mycobacterium spp. isolates from Yemeni tuberculosis patients

Abstract

This study was done to characterize at the species level Mycobacterium spp. isolates from Yemeni pulmonary tuberculosis patients. Early-morning sputum samples were collected from 170 patients referred to the National Tuberculosis Institute in Sana'a city with suspected pulmonary tuberculosis. Samples were processed with Ziehl-Neelsen stain and cultured in Ogawa and Lowenstein-Jensen media. The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by HindIII enzyme digestion. Of the 120 isolates analysed, 118 (98.3%) were identified as M. tuberculosis complex and 2 (1.7%) were identified as mycobacteria other than M. tuberculosis. The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex.

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

“…The remaining three articles reported A. baumannii , Pseudomonas aeruginosa , and S. aureus . All the bacterial isolates in these articles were from human sources, including 120 strains of M. tuberculosis [ 115 ], 60 of S. aureus [ 270 ], and 65 of P. aeruginosa [ 237 ]. As mentioned, the number of reports from Yemen were generally fewer compared to the other countries studied; three research articles mentioned the isolation of M. tuberculosis from patients residing in tuberculosis (TB) centers in Yemen.…”

“…In a similar study was conducted by the same author, in which MDR M. tuberculosis involving 135 patients was explored to evaluate health-related risk factors [ 16 ]. Furthermore, sputum samples were collected from 170 patients in the National TB Institute of Sana’a and 118 M. tuberculosis isolates were identified [ 115 ]. Two articles, both written by Jaber et al, reported mortality data in Yemen [ 16 , 17 ].…”