Molecular Typing of Methicillin Resistant Staphylococcus aureus Colonizing Egyptian Healthcare Workers and Patients

Mahdi, Wafaa K.M.; Hassuna, Noha A.; Esmail, Mona Abdel Monem; Hammad, Safaa S.; Abdelwahab, Sayed F.

doi:10.20546/ijcmas.2016.506.075

Cited by 1 publication

(2 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…Other studies have suggested that methicillin resistance can occur in the absence of mecA and that MRSA may possess additional resistance mechanisms, such as changed target sites or possibly decreased drug accumulation. The mecA absence could result from a detection error [48]. The potential of MRSA strains to develop resistance to antimicrobial treatment makes them cause major nosocomial diseases and spread worldwide in recent years [49].…”

Section: Ability Of S Aureus To Produce Virulence Factors (Meca Detec...mentioning

confidence: 99%

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Effectiveness of raw bacteriocin produced from lactic acid bacteria on biofilm of methicillin-resistant Staphylococcus aureus

et al. 2023

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Background and Aim: Probiotics are proven beneficial to health since they enhance immunity against dangerous pathogens and increase resistance to illness. Bacteriocin produced by lactic acid bacteria (LAB), demonstrates a broad inhibitory spectrum and therapeutic potential. This study aimed to isolate LAB-producing bacteriocin and investigate the effect of crude bacteriocin on biofilm from methicillin-resistant Staphylococcus aureus (MRSA). Materials and Methods: This study used randomly collected 80 white soft local cheeses (40 each from cows and sheep) from different supermarkets in Basrah Province. The obtained samples were cultured and the bacterial suspension of S. aureus was prepared at 1.5 × 108 cells/mL. The crude bacteriocin extracted from LAB was obtained, and the tube was dried and inverted to detect the biofilm loss at the bottom. Results: There were 67 (83.75%) LAB isolates. Among 40 milk samples collected directly and indirectly, there were 36 (83.33%). Staphylococcus aureus isolates based on conventional bacteriological analysis and biochemical tests. Molecular testing was conducted to identify LAB and MRSA. Depending on genotypic results, the effect of white soft local cheese (cows and sheep) and the amplification results of the 16S rRNA gene were detected in 46 LAB isolates from white soft local cheese from cows and sheep. Based on the molecular identification of the mecA, results on Staphylococcus determined that only 2 of 36 isolates of S. aureus carried the mecA. Moreover, there were 26 (86.66%) isolates (MRSA) from samples of raw milk from local markets and subclinical mastitis in cows. The ability of LAB isolates was tested. The effects of bacteriocin production on preventing biofilm growth and formation were investigated. Results demonstrated that bacteriocin has high activity. Microtiter plates applied to investigate the ability of S. aureus to produce biofilms revealed that all isolates were either weak or moderate biofilm producers, with neither non-biofilm nor strong biofilm producers found among the tested isolates. Conclusion: Lactic acid bacteria demonstrate a high ability to produce bacteriocin. Crude bacteriocin from LAB has a restrictive effect on biofilms produced by MRSA; thus, it can be used to reduce the pathogenicity of this bacterium. Keywords: 16S rRNA gene, biofilm formation assay, mecA gene, polymerase chain reaction, subclinical mastitis.

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Section: Discussionmentioning

confidence: 99%

Section: Ability Of S Aureus To Produce Virulence Factors (Meca Detec...mentioning

confidence: 99%

Effectiveness of raw bacteriocin produced from lactic acid bacteria on biofilm of methicillin-resistant Staphylococcus aureus

et al. 2023

View full text Add to dashboard Cite

show abstract

Molecular Typing of Methicillin Resistant Staphylococcus aureus Colonizing Egyptian Healthcare Workers and Patients

Cited by 1 publication

References 12 publications

Effectiveness of raw bacteriocin produced from lactic acid bacteria on biofilm of methicillin-resistant Staphylococcus aureus

Effectiveness of raw bacteriocin produced from lactic acid bacteria on biofilm of methicillin-resistant Staphylococcus aureus

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