Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis

Lawrence, Christine; Ronco, E; Dubrou, S.; Leclercq, Roland; Nauciel, C; Matsiota-Bernard, P.

doi:10.1099/00222615-48-4-327

Cited by 25 publications

(16 citation statements)

References 17 publications

(5 reference statements)

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“…This might constitute a confounding factor in the attribution of clonality. Although the results obtained by PFGE were overall in agreement with those obtained by MAb typing, the isolates of L. pneumophila with the same patterns might not come from the same environmental source (18). Therefore, these results, even if they are reliable (16,22), should be used with caution (7,25).…”

supporting

confidence: 72%

Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

et al. 2005

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In the summer of 2003 a community-acquired outbreak of Legionella pneumophila occurred in Rome, Italy. Three molecular typing methods, pulse-field gel electrophoresis, amplified fragment length polymorphism analysis, and sequence-based typing (SBT), were used to establish the clonal correlation among the isolates of the epidemic cluster. By comparison of the methods, SBT was the most rapid and the easiest to perform and provided unambiguous results.

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supporting

confidence: 72%

Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

et al. 2005

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“…This method has been useful and rapid but is less discriminatory than recent genotyping methods. Among the latter, PFGE subsequent to macrorestriction has been shown to be an effective method for genotyping Legionellae (15,19,26,30,35). However, some laboratories may not have the requisite equipment.…”

Section: Discussionmentioning

confidence: 99%

“…Several genotypic methods have also been developed and used for epidemiological investigations, in some cases together with MAb subtyping (10,15,17,19,26,27,28,30,32,35). The European Working Group on Legionella Infections (EWGLI [www.ewgli.org]) studied the molecular methods that were currently in use and the collaboration group found two methodsmacrorestriction followed by pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism analysis (AFLP)-to be the two most useful (7).…”

mentioning

confidence: 99%

A Hospital-Associated Outbreak of Legionnaires' Disease Caused by Legionella pneumophila Serogroup 1 Is Characterized by Stable Genetic Fingerprinting but Variable Monoclonal Antibody Patterns

et al. 2003

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An outbreak of 18 pneumonia cases caused by Legionella pneumophila serogroup 1 occurred at a Swedish university hospital 1996 to 1999. Eight clinical isolates obtained by culture from the respiratory tract were compared to 20 environmental isolates from the hospital and to 21 epidemiologically unrelated isolates in Sweden, mostly from patients, by using pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism analysis (AFLP), and monoclonal antibody (MAb) typing. All patients and most environmental isolates from the outbreak hospital belonged to the same genotypic cluster in both PFGE and AFLP. This genotype was distinctly different from other strains, including a cluster from a second hospital in a different part of the country. The MAb subtype of the outbreak clone was Knoxville except for three isolates that were Oxford. A variation in the MAb reactivity pattern was also found in a second genotypic cluster. These changes in the MAb reactivity pattern were due to the absence or presence of the lag-1 gene coding for an Oacetyltransferase that is responsible for expression of the lipopolysaccharide epitope recognized by MAb 3/1 of the Dresden Panel. In all MAb 3/1-positive strains, the lag-1 gene was present on a genetic element that was bordered by a direct repeat that showed a high degree of sequence homology. Due to this homology, the lag-1 gene region seemed to be an unstable element in the chromosome. MAb patterns are thus a valuable adjunct to genotyping methods in defining subgroups inside a genotypic cluster of L. pneumophila sg 1.

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“…Other procedures used to subtype L. pneumophila include detection of repetitive elements within DNA, amplified fragment length polymorphism, and sequence-based typing (gyrA gene) (56,86,113,116,176,177,197,274,276).…”

Section: Subtyping Of Legionella Sppmentioning

confidence: 99%

Legionella and Legionnaires' Disease: 25 Years of Investigation

2002

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There is still a low level of clinical awareness regarding Legionnaires' disease 25 years after it was first detected. The causative agents, legionellae, are freshwater bacteria with a fascinating ecology. These bacteria are intracellular pathogens of freshwater protozoa and utilize a similar mechanism to infect human phagocytic cells. There have been major advances in delineating the pathogenesis of legionellae through the identification of genes which allow the organism to bypass the endocytic pathways of both protozoan and human cells. Other bacteria that may share this novel infectious process are Coxiella burnetti and Brucella spp. More than 40 species and numerous serogroups of legionellae have been identified. Most diagnostic tests are directed at the species that causes most of the reported human cases of legionellosis, L. pneumophila serogroup 1. For this reason, information on the incidence of human respiratory disease attributable to other species and serogroups of legionellae is lacking. Improvements in diagnostic tests such as the urine antigen assay have inadvertently caused a decrease in the use of culture to detect infection, resulting in incomplete surveillance for legionellosis. Large, focal outbreaks of Legionnaires' disease continue to occur worldwide, and there is a critical need for surveillance for travel-related legionellosis in the United States. There is optimism that newly developed guidelines and water treatment practices can greatly reduce the incidence of this preventable illness

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Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis

Cited by 25 publications

References 17 publications

Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

Comparison of Three Molecular Methods Used for Subtyping of Legionella pneumophila Strains Isolated during an Epidemic of Legionellosis in Rome

A Hospital-Associated Outbreak of Legionnaires' Disease Caused by Legionella pneumophila Serogroup 1 Is Characterized by Stable Genetic Fingerprinting but Variable Monoclonal Antibody Patterns

Legionella and Legionnaires' Disease: 25 Years of Investigation

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