Molecular Typing of
            <i>Vibrio parahaemolyticus</i>
            Isolates, Obtained from Patients Involved in Food Poisoning Outbreaks in Taiwan, by Random Amplified Polymorphic DNA Analysis

Wong, Hin-Chung; Liu, Chi-Chang; Pan, Tze-Ming; Wang, Tien-Kuei; Lee, Chih-Lung; Shih, Daniel Yang-Chih

doi:10.1128/jcm.37.6.1809-1812.1999

Cited by 35 publications

(16 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to considerable sequence similarity among the 16S rRNA gene sequences, an alternate method was employed to investigate the genetic diversity among the Pseudovibrio isolates. Using RAPD, which is frequently used to determine the genetic relationship between closely related bacterial species (Wong et al 1999;De Cesare et al 2001;Sudheesh et al 2002), 33 different RAPD types were identified among the 73 Pseudovibrio isolates, indicating that there is a genetically diverse population of Pseudovibrio spp. present which was not reflected in the 16S rRNA gene sequence analysis.…”

Section: Discussionmentioning

confidence: 99%

Diversity and antimicrobial activity of Pseudovibrio spp. from Irish marine sponges

Halloran

Barbosa

Morrissey

et al. 2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To evaluate the diversity and antimicrobial activity present among Pseudovibrio spp. isolated from marine sponges. Methods and Results: Seventy‐three bacterial isolates from the marine sponges Polymastia boletiformis, Axinella dissimilis and Haliclona simulans were identified as Pseudovibrio spp. using phylogenetic analysis of 16S rRNA gene sequences. Genetic diversity among these isolates was estimated using random amplification of polymorphic DNA (RAPD), and 33 RAPD types were identified among the 73 Pseudovibrio isolates. These Pseudovibrio spp. were assayed for the production of compounds with antimicrobial activity against various clinically relevant pathogens. Sixty‐two (85%) of the isolates showed activity against at least one of the pathogens tested, including Escherichia coli, Salmonella enterica serotype Typhimurium, methicillin‐resistant Staphylococcus aureus (MRSA), and Clostridium difficile. PCR screens of the Pseudovibrio isolates also revealed the presence of potential antibiotic‐producing polyketide synthase genes. Conclusions: Marine sponges harbour a diverse population of Pseudovibrio spp., the majority of which demonstrate antimicrobial activity. The identification of several different antimicrobial activity spectra suggests that the Pseudovibrio isolates may produce a suite of antimicrobial compounds. Significance and Impact of the Study: This is the first study in which an extended population of Pseudovibrio isolates from marine sponges has been analysed and establishes the little‐studied Pseudovibrio as a potentially important genus in the search for antimicrobial compounds of clinical relevance.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity and antimicrobial activity of Pseudovibrio spp. from Irish marine sponges

Halloran

Barbosa

Morrissey

et al. 2011

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Unlike PCR, RAPD does not require a known target sequence for its operation. A 10-mer primer (5¢-CAG GCG CAC A-3¢) was selected to generate polymorphic amplification profiles of V. parahaemolyticus genomic DNA of 308 clinical and environmental isolates of V. parahaemolyticus by the RAPD method, generating 41 patterns that were grouped into 16 types (A-P) (Wong et al, 1999c). Several groups have used a variation of RAPD known as arbitrarily primed PCR to type urease-positive strains of V. parahaemolyticus (Okuda et al, 1997a) and the pandemic O3:K6 strains (Okuda et al, 1997b).…”

Section: Typing Methodsmentioning

confidence: 99%

A review of the current status of cultural and rapid detection of Vibrio parahaemolyticus

Bisha

Simonson

Janes

et al. 2012

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary Vibrio parahaemolyticus is ubiquitous in estuarine environments and can be commonly found in seafood products. This bacterial pathogen continues to emerge as an important cause of foodborne illness, and several foodborne disease outbreaks caused by V. parahaemolyticus have been linked to the consumption of contaminated seafood, in particular those consumed raw such as oysters. In response to these outbreaks, especially during the 1990s, several cultural, immunological‐based and molecular detection methods have been developed, which allow for rapid detection and quantification of total and pathogenic V. parahaemolyticus. The development of molecular methodology has allowed for clinical and environmental isolates of V. parahaemolyticus to be subtyped, thus providing the framework for risk‐based strategies aimed at controlling foodborne outbreaks cause by this pathogen. It is important that the detection and typing methods strive to accomplish detection and differentiation of the pathogenic strains from environmental (non‐pathogenic) ones, as well as to detect the presence of the organism and not just the presence of V. parahaemolyticus produced toxins, which can also be produced by closely related species. This review covers the current status of detection and typing methodology for identification and characterisation of V. parahaemolyticus from seafood.

show abstract

“…2003). Illnesses have been reported from a number of countries including Taiwan, Japan, USA and India (Wong et al. 1999).…”

Section: Introductionmentioning

confidence: 99%

“…2004), ribotyping (Wong et al. 1999), random amplified polymorphic DNA (RAPD) analysis (Wong et al. 1999; Lu et al.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular typing of Vibrio parahaemolyticus isolated from seafood harvested along the south-west coast of India

Bhowmick

Khushiramani

Raghunath

et al. 2007

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aims: Evaluation of protein profiling for typing Vibrio parahaemolyticus using 71 strains isolated from different seafood and comparison with other molecular typing techniques such as random amplified polymorphic DNA analysis (RAPD) and enterobacterial repetitive intergenic consensus sequence (ERIC)‐PCR. Methods and Results: Three molecular typing methods were used for the typing of 71 V. parahaemolyticus isolates from seafood. RAPD had a discriminatory index (DI) of 0·95, while ERIC‐PCR showed a DI of 0·94. Though protein profiling had less discriminatory power, use of this method can be helpful in identifying new proteins which might have a role in establishment in the host or virulence of the organism. Conclusions: The use of protein profiling in combination with other established typing methods such as RAPD and ERIC‐PCR generates useful information in the case of V. parahaemolyticus associated with seafood. Significance and Impact of the Study: The study demonstrates the usefulness of nucleic acid and protein‐based studies in understanding the relationship between various isolates from seafood.

show abstract

Molecular Typing of Vibrio parahaemolyticus Isolates, Obtained from Patients Involved in Food Poisoning Outbreaks in Taiwan, by Random Amplified Polymorphic DNA Analysis

Cited by 35 publications

References 7 publications

Diversity and antimicrobial activity of Pseudovibrio spp. from Irish marine sponges

Diversity and antimicrobial activity of Pseudovibrio spp. from Irish marine sponges

A review of the current status of cultural and rapid detection of Vibrio parahaemolyticus

Molecular typing of Vibrio parahaemolyticus isolated from seafood harvested along the south-west coast of India

Contact Info

Product

Resources

About