Molecular typing of <i><scp>N</scp>eisseria perflava</i> clinical isolates

Mechergui, Arij; Achour, Wafa; Giorgini, Dario; Baaboura, Rekaya; Taha, Muhamed‐Kheir; Hassen, Assia Ben

doi:10.1111/apm.12042

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…The digestion of genomic DNA with enzymes such as SpeI has proven to be highly discriminatory for N. perflava [5]. This is in accordance with a previous study [27], which found that PFGE is able to discriminate nonpathogenic Neisseria spp.…”

Section: Plasmid Analysissupporting

confidence: 90%

“…MLST can type the commensal N. perflava using sequencing of seven housekeeping gene fragments: abcZ, adk, aroE, gdh, pdhC, fumC and pgm [5]. It revealed 22 different allelic profiles among 22 N. perflava clinical isolates collected by swabbing from neutropenic patients, with an extensive heterogeneity in the alleles identified among N. perflava isolates [5].…”

Section: S Rrna Sequencingmentioning

confidence: 99%

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Principles and applications of typing methods for commensal Neisseria

Mechergui¹,

Achour²,

Hassen³

2015

Reviews in Medical Microbiology

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This review describes the design as well as advantages and disadvantages for current phenotypic (antibiogram and serotyping) and molecular techniques (plasmid analysis, arbitrarily primed PCR, 16S rRNA, pulse-field gel electrophoresis and multilocus sequence typing) for typing of commensal Neisseria spp. compared with pathogenic Neisseria species. Identical methods for typing Neisseria species should not be used in all situations, that is, for microepidemiological, macroepidemiological or evolutionary questions.

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Section: Plasmid Analysissupporting

confidence: 90%

Section: S Rrna Sequencingmentioning

confidence: 99%

Principles and applications of typing methods for commensal Neisseria

Mechergui¹,

Achour²,

Hassen³

2015

Reviews in Medical Microbiology

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“…Indeed, Neisseria species are naturally competent, and inter‐ and intraspecific horizontal gene transfer has been described . Also, a genetic diversity is found among the MLST housekeeping genes of the commensal N. perflava .…”

Section: Discussionmentioning

confidence: 99%

Intraspecific 16S rRNA gene diversity among clinical isolates ofNeisseriaspecies

Mechergui

Achour

Hassen

2013

APMIS

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In the present work, nearly the entire 16S rRNA gene sequences of 46 clinical samples of Neisseria spp. were determined, and the aligned sequences were analyzed to investigate the diversity of 16S rRNA genes in each commensal Neisseria species. Two 16S rRNA types were identified in two Neisseria sicca strains, three 16S rRNA types in five Neisseria macacae strains, fourteen 16S rRNA types in twenty Neisseria flavescens isolates, and fourteen 16S rRNA types in nineteen Neisseria mucosa isolates. The number of nucleotides that were different between 16S rRNA sequences within specie ranged from 1 to 15. We found high intraspecific sequence variation in 16S rRNA genes of Neisseria spp. strains.

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“…PFGE was done as described previously by Mechergui et al . Our 46 isolates were digested with Spe I, and the resulting fragments were separated by electrophoresis in CHEFF DR II (Biorad) in 1% MP agarose gel (Boehringer Mannheim) made with 0.5× TBE (Tris Borate 45 mmol/L/EDTA 1 mmol/L pH 8).…”

Section: Methodsmentioning

confidence: 99%

Genotyping of commensal Neisseria spp strains by pulsed‐field gel electrophoresis and 16S rRNA gene sequencing

Mechergui¹,

Achour²,

Hassen³

2017

Clinical Laboratory Analysis

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Background We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis. Methods Identification to the genus was done using conventional methods and API NH (bio‐Mérieux®). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI. Results Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty‐three different PFGE patterns were found among the tested strains. Conclusion We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.

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Molecular typing of Neisseria perflava clinical isolates

Abstract: Multilocus sequence typing and pulsed-field gel electrophoresis were used to type 22 commensal isolates of Neisseria perflava collected by swabbing from neutropenic patients. High genetic diversity was found among our N. perflava clinical isolates.

Cited by 4 publications

References 16 publications

Principles and applications of typing methods for commensal Neisseria

Principles and applications of typing methods for commensal Neisseria

Intraspecific 16S rRNA gene diversity among clinical isolates ofNeisseriaspecies

Genotyping of commensal Neisseria spp strains by pulsed‐field gel electrophoresis and 16S rRNA gene sequencing

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