Molecular Typing of <i>Agrobacterium</i> Species Isolates From Catheter-Related Bloodstream Infections

Giammanco, Giovanni M.; Pignato, Sarina; Santangelo, Carmelita; Grimont, Patrick A. D.; Grimont, Francine; Giammanco, G

doi:10.1086/502315

Cited by 16 publications

(16 citation statements)

References 11 publications

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“…For epidemiological purposes, PFGE-RFLP appeared more discriminative than MLST, since it was able to differentiate strains belonging to the same multilocus sequence type and is probably a suitable method for the epidemiological follow-up of human outbreaks due to Agrobacterium. PFGE previously permitted detection of the nosocomial transmission of Agrobacterium isolates from intravenous catheters of two hospitalized patients (17).…”

Section: Discussionmentioning

confidence: 99%

“…In the past 2 decades, Agrobacterium (Rhizobium) radiobacter has been recognized as an opportunistic human pathogen responsible for nosocomial infections, mainly bacteremia, peritonitis, and urinary tract infections (1,3,10,28). Bacteremias are usually secondary to the use of intravenous devices (3,11,17). A. radiobacter strains have also been found in the respiratory tracts of cystic fibrosis patients (5).…”

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confidence: 99%

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Multilocus Sequence-Based Analysis Delineates a Clonal Population of Agrobacterium (Rhizobium) radiobacter (Agrobacterium tumefaciens) of Human Origin

et al. 2011

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The genus Agrobacterium includes plant-associated bacteria and opportunistic human pathogens. Taxonomy and nomenclature within the genus remain controversial. In particular, isolates of human origin were all affiliated with the species Agrobacterium (Rhizobium) radiobacter, while phytopathogenic strains were designated under the synonym denomination Agrobacterium tumefaciens. In order to study the relative distribution of Agrobacterium strains according to their origins, we performed a multilocus sequence-based analysis (MLSA) on a large collection of 89 clinical and environmental strains from various origins. We proposed an MLSA scheme based on the partial sequence of 7 housekeeping genes (atpD, zwf, trpE, groEL, dnaK, glnA, and rpoB) present on the circular chromosome of A. tumefaciens C58. Multilocus phylogeny revealed that 88% of the clinical strains belong to genovar A7, which formed a homogeneous population with linkage disequilibrium, suggesting a low rate of recombination. Comparison of genomic fingerprints obtained by pulsed-field gel electrophoresis (PFGE) showed that the strains of genovar A7 were epidemiologically unrelated. We present genetic evidence that genovar A7 may constitute a human-associated population distinct from the environmental population. Also, phenotypic characteristics, such as culture at 42°C, agree with this statement. This human-associated population might represent a potential novel species in the genus Agrobacterium.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Multilocus Sequence-Based Analysis Delineates a Clonal Population of Agrobacterium (Rhizobium) radiobacter (Agrobacterium tumefaciens) of Human Origin

et al. 2011

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“…In the years since, A. tumefaciens transformation has also been achieved (with mixed success) in many other eukaryotic hosts. Cases of Agrobacterium infection and proliferation have been observed in human patients, suggesting that Agrobacterium may have some clinical potential as a gene delivery vector [179,180]. However, tail vein injections of A. tumefaciens in mice yielded no transgene expression, even though the bacteria remained viable [181].…”

Section: Parasitic Hgt: a Tumefaciensmentioning

confidence: 99%

Applying horizontal gene transfer phenomena to enhance non-viral gene therapy

Elmer

Christensen

Rege

2013

Journal of Controlled Release

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Horizontal gene transfer (HGT) is widespread amongst prokaryotes, but eukaryotes tend to be far less promiscuous with their genetic information. However, several examples of HGT from pathogens into eukaryotic cells have been discovered and mimicked to improve non-viral gene delivery techniques. For example, several viral proteins and DNA sequences have been used to significantly increase cytoplasmic and nuclear gene delivery. Plant genetic engineering is routinely performed with the pathogenic bacterium Agrobacterium tumefaciens and similar pathogens (e.g. Bartonella henselae) may also be able to transform human cells. Intracellular parasites like Trypanosoma cruzi may also provide new insights into overcoming cellular barriers to gene delivery. Finally, intercellular nucleic acid transfer between host cells will also be briefly discussed. This article will review the unique characteristics of several different viruses and microbes and discuss how their traits have been successfully applied to improve non-viral gene delivery techniques. Consequently, pathogenic traits that originally caused diseases may eventually be used to treat many genetic diseases.

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“…Agrobacterium is also considered to be a rare and opportunistic human pathogen [48][49][50]. In the isolates of human patients affected by bacteremia, peritonitis, and endocarditis, Agrobacterium radiobacter, a virulent Agrobacterium sp.…”

Section: Rhizobia Mediated Plant Transformationmentioning

confidence: 99%

“…was found to be the causative agent of the disease [49]. In addition, two other Agrobacterium sp., A. tumifaciens and Agrobacteruim vilis were isolated from hospitilized patients [50], but no measures were undertaken to determine Agrobacterium as the causative agent. In 2007, Agrobacterium was associated with Morgellons syndrome [51].…”

Section: Rhizobia Mediated Plant Transformationmentioning

confidence: 99%

Rhizobia species: A Boon for “Plant Genetic Engineering”

Patel¹,

Sinha²

2011

Indian J Microbiol

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Since past three decades new discoveries in plant genetic engineering have shown remarkable potentials for crop improvement. Agrobacterium Ti plasmid based DNA transfer is no longer the only efficient way of introducing agronomically important genes into plants. Recent studies have explored a novel plant genetic engineering tool, Rhizobia sp., as an alternative to Agrobacterium, thereby expanding the choice of bacterial species in agricultural plant biotechnology. Rhizobia sp. serve as an open license source with no major restrictions in plant biotechnology and help broaden the spectrum for plant biotechnologists with respect to the use of gene transfer vehicles in plants. New efficient transgenic plants can be produced by transferring genes of interest using binary vector carrying Rhizobia sp. Studies focusing on the interactions of Rhizobia sp. with their hosts, for stable and transient transformation and expression of genes, could help in the development of an adequate gene transfer vehicle. Along with being biologically beneficial, it may also bring a new means for fast economic development of transgenic plants, thus giving rise to a new era in plant biotechnology, viz. "Rhizobia mediated transformation technology."

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Molecular Typing of Agrobacterium Species Isolates From Catheter-Related Bloodstream Infections

Cited by 16 publications

References 11 publications

Multilocus Sequence-Based Analysis Delineates a Clonal Population of Agrobacterium (Rhizobium) radiobacter (Agrobacterium tumefaciens) of Human Origin

Multilocus Sequence-Based Analysis Delineates a Clonal Population of Agrobacterium (Rhizobium) radiobacter (Agrobacterium tumefaciens) of Human Origin

Applying horizontal gene transfer phenomena to enhance non-viral gene therapy

Rhizobia species: A Boon for “Plant Genetic Engineering”

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