Molecular typing of colonizing Streptococcus agalactiae strains by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) in a Chennai based hospital

Bishi, Dillip Kumar; Verghese, Susan T.; Verma, Rama Shanker

doi:10.1007/s12088-008-0017-2

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…ERIC-PCR-based genotyping method is reproducible and as discriminative as pulsed field gel electrophoresis. It is fast, cheap, and requires no speJournal of Poultry Science,55 (1) cialized equipment or reagents (Bishi et al, 2008;Kosek et al, 2012). In this study, two Salmonella-positive overshoe samples collected from one person on farm A yielded an identical gel pattern, further demonstrating the reliability and accuracy of the ERIC-PCR.…”

Section: Eric-pcr Fingerprinting and Clustering Analysissupporting

confidence: 51%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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Salmonella is the most common cause of foodborne illnesses worldwide. Poultry eggs are a major contamination source of Salmonella. The prevalence of Salmonella has been effectively reduced since a series of measures were taken to reduce contamination in egg-laying houses. In the present study, 1,512 environmental samples obtained from layer farms of different production scales were screened in a voluntary Salmonella survey study. Contaminations were detected using a PCR method. Genetic relationships among Salmonella samples were specified using molecular typing by enterobacterial repetitive intergenic consensus (ERIC)-PCR. The survey results showed that two layer farms, located in the Shandong and Hebei provinces, were contaminated with Salmonella. Thirty-one samples from these two farms, including feed, drinking nipples, egg collection belt, air inlets and outlets, air, overshoes, and eggshells, were identified as Salmonella-positive. It was observed that certain samples within the henhouses as well as in the egg collecting areas showed relatively high genetic similarities. The survey conclusively revealed minor Salmonella contamination in northern China. Moreover, various areas within the layer farms were identified as part of the propagation chain of Salmonella. Furthermore, evidence of cross-contamination of Salmonella was found in the laying houses and egg collection areas, even between these two regions. Therefore, it is necessary to establish routine Salmonella detection and subsequent environmental control measures in order to decrease the prevalence of Salmonella.

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Section: Eric-pcr Fingerprinting and Clustering Analysissupporting

confidence: 51%

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Liu

et al. 2018

J. Poult. Sci.

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“…ERIC-PCR: DNA of GBS isolates was extracted using phenol-chloroform method as previously described [26] . ERIC PCR was done with ERIC primers consisting of ERIC 1 (5'-ATG TAAGCTCCTGGGGATTCAC-3') and ERIC 2 (5'-AAG TAA GTG ACT GGG GTG AGCG-3') [27] . PCR program was carried out with a pre-denaturation step at 95°C for 5 min, followed by 30 cycles of denaturation (at 95°C for 60 s, 59°C for 50 s, and 72°C for 60 s) and a final extension at 72°C for 10 min [28] .…”

Section: Methodsmentioning

confidence: 99%

Molecular Typing of Streptococcus agalactiae- cMLSB Phenotype Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) in Isfahan, Iran

Jalalifar

Motallebirad²,

Dashtbin³

et al. 2022

IEM

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Backgrounds: Group B Streptococcus (GBS) is an important opportunistic bacterial pathogen that could cause serious infections, especially in neonates, adults, and the elderly. In GBS isolates, a macrolide resistance phenotype that confers constitutive resistance to macrolidelincosamide-streptogramin B antibiotics (cMLSB phenotype) has become a global concern. On the other hand, little is known about the genetic relatedness and diversity of GBS isolates isolated from various patients in Iran. Hence, this study aimed to determine the genetic relatedness and molecular typing of cMLSB-GBS isolates using enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) technique. Materials & Methods: A total of 100 GBS isolates were collected from patients with urinary tract infections (UTIs). Among them, 52 erythromycin-resistant GBS isolates were selected, and double-disc diffusion (D-zone) technique was applied to determine the MLSB phenotype among the isolates based on CLSI criteria. Then the genetic relatedness of MLSB-GBS isolates was assessed using ERIC-PCR fingerprinting method. Findings: Among 52 erythromycin-resistant GBS isolates, 38 isolates were identified with cMLSB phenotype, nine isolates with M phenotype, and five isolates with iMLSB phenotype. The analysis of ERIC-PCR patterns revealed eight different ERIC types that were divided into seven clusters (A-G) and one single type. Also, four isolates were non-typeable. ERIC type A/ serotype Ib was the most prevalent clone among the isolates. Conclusion:The current study findings showed a high level of diversity and multiclonal spread of the cMLSB phenotype in Isfahan. ERIC type A/ serotype Ib is the predominant clone circulating among erythromycin-resistant GBS strains.

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“…(Chen et al, ; Leal, Queiroz, Pereira, Tavares, & Figueiredo, ). ERIC‐PCR is a method of simple operation, low cost, rapid, effective discrimination and repetitiveness; it is valuable for bacterial genotyping (Bishi, Verghese, & Verma, ). The MLST is considered as a highly sensitive technique for determining the evolutionary history and epidemiology of pathogens.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Streptococcus spp. isolated from tilapia (Oreochromis spp.) cultured in river‐based cage and earthen ponds in Northern Thailand

Niu

Khattiya

Zhang

et al. 2020

Journal of Fish Diseases

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Streptococcus spp. are major pathogenic bacteria associated with massive mortality in tilapia. This study investigated the phenotypic and genotypic characterization of Streptococcus agalactiae (GBS) and Streptococcus iniae (S. iniae) isolated from tilapia in river-based floating cage and earthen pond farms in northern Thailand. Isolates were identified by biochemical and molecular analyses. Capsular typing, enterobacterial repetitive intergenic consensus polymerase chain reaction and multilocus sequence typing were performed to investigate the genetic relatedness. Six and one isolates were confirmed as GBS and S. iniae, respectively. All Streptococcus spp. isolates were obtained from 4 river-based cage farms (4/33), while samples collected from earthen pond farms (N = 28) were negative for streptococcosis. All GBS with serotype Ⅲ and sequence type (ST) 283 was observed. The β-haemolytic GBS isolates were resistant to five antimicrobials, while the S. iniae was susceptible to all antimicrobials. This study indicates both GBS and S. iniae are the major bacterial pathogens responsible for streptococcosis infection in farmed tilapia of northern Thailand with GBS as dominant species. This survey highlights that the river-based cage farms seriously impact on the healthy development of the tilapia industry. K E Y W O R D Searthen pond farm, northern Thailand, river-based cage farm, Streptococcus spp., tilapia ACK N OWLED G EM ENTS

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Molecular typing of colonizing Streptococcus agalactiae strains by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) in a Chennai based hospital

Cited by 7 publications

References 21 publications

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

<i>Salmonella</i> Contamination in Layer Farms in China: Detection and Genetic Analysis

Molecular Typing of Streptococcus agalactiae- cMLSB Phenotype Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) in Isfahan, Iran

Phenotypic and genotypic characterization of Streptococcus spp. isolated from tilapia (Oreochromis spp.) cultured in river‐based cage and earthen ponds in Northern Thailand

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