Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR

Erol, İrfan; Göncüoğlu, Muammer; Ayaz, Naim Deniz; Ormanci, Fatma Seda Bilir; Hildebrandt, G.

doi:10.1111/j.1472-765x.2008.02379.x

Cited by 35 publications

(24 citation statements)

References 21 publications

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“…C. perfringens types prevalent in poultry meat and meat parts conducted in Turkey were similar to those found in our study (8,11,15). Although there are no published data on detection of C. perfringens in poultry edible offal samples, previous studies in Turkey showed that edible offal of broiler can be contaminated with different foodborne pathogens mainly including Salmonella spp.…”

Section: Discussionsupporting

confidence: 74%

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Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

GÖNCÜOĞLU¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to determine the prevalance and molecular characterization of Clostridium perfringens in edible offal of broiler. For this purpose a total of 90 samples, including 45 heart-livers and 45 gizzards were obtained from different supermarkets and transported to the laboratory in an ice box and tested at the same day. Culture technique was used for the isolation and selected biochemical tests, including acid phosphatase and reverse-CAMP tests was used for the identification of C. perfringens from the samples. Twenty one (46.7%) of 45 heart-liver and eight (17.7%) of 45 gizzards were found to be contaminated with C. perfringens. Isolates were molecular characterized by using multiplex PCR with alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) genes. Results showed that all isolates were carrying cpa gene and named as type A but none of them were positive for cpe gene. Only two gizzard isolates were positive for cpb2 gene. Our results indicate that edible offal of broiler are highly contaminated with C. perfringens type A which is mostly responsible for the foodborne poisoning worldwide. According to the high contamination rate edible offal should be considered as one of the important causes of C. perfringens type A foodborne disease.Key words: Broiler edible offal, Clostridium perfringens, molecular typing, multiplex PCR. Yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve moleküler tiplendirilmesiÖzet: Bu çalışmada yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve elde edilen izolatların moleküler karakterizasyonlarının belirlenmesi amaçlanmıştır. Çalışmada farklı süpermarketlerde satışa sunulan 45 kalp-karaciğer ve 45 taşlık olmak üzere toplam 90 yenilebilir iç organ soğuk zincir altında laboratuvara getirilerek aynı gün içerisinde analize alınmıştır. Örneklerden C. perfringens izolasyon ve identifikasyonunda klasik kültür tekniği ile asit fosfataz ve reverse-CAMP gibi biyokimyasal testler kullanılmıştır. Kırk beş kalp-karaciğer örneğinin 21'inin (% 46.7) ve 45 taşlık örneğinin sekizinin (% 17.7) C. perfringens ile kontamine olduğu saptanmıştır. Elde edilen izolatların alfa (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) ve enterotoksin (cpe) genlerine ait primer dizilimleri kullanılarak multipleks PCR ile moleküler tiplendirilmeleri yapılmıştır. Sonuçlar incelendiğinde bütün izolatların cpa geni taşıdığı belirlenmiş ve tip A olarak sınıflandırılmıştır, bununla beraber hiçbir izolatın cpe genine sahip olmadığı saptanmıştır. İki farklı taşlık örneğinden elde edilen izolatların cpa yanında cpb2 genini de taşıdıkları belirlenmiştir. Sonuç olarak yenilebilir piliç iç organlarının dünya genelinde gıda zehirlenmelerinde en sıklıkla rastlanan C. perfringens tip A ile yüksek oranda kontamine olduğu belirlenmiştir. Yüksek kontaminasyona bağlı olarak yenilebilir piliç iç organlarının C. perfringens tip A zehirlenmeleri açısından önemli birer kaynak oldukları saptanmıştır. Anahtar sözcükler: Clostridium per...

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Section: Discussionsupporting

confidence: 74%

“…The suspensions were mixed and heated at 95°C for 20 min in a water bath (Memmert WB⁄OB 7-45, WBU 45, Schwabagh, Germany) and centrifuged for 3 min at 12 000 g and cooled on ice. A volume of 10 μl was used as a template in the PCR (11).…”

Section: Methodsmentioning

confidence: 99%

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

GÖNCÜOĞLU¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…In a study conducted by Erol et al [32], it was found that 12.2% of the turkey meat samples was contaminated by C. perfringens. Sarıgüzel and Erol [33] isolated and identified C. perfringens in 58 of 100 turkey minced meat samples.…”

Section: Resultsmentioning

confidence: 99%

A Study about Microbiological Quality and Species Identification of Frozen Turkey Meat

Vural

Erkan²,

Güran³

et al. 2013

IJNFS

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Abstract:The aim of this study was to identify potential health risks and evaluate the microbiological quality of frozen turkey meat sold in Diyarbakır, Turkey. In this study, a total of 110 frozen turkey meat samples were collected from retail markets in the city of Diyarbakır. The samples were analyzed by using standard analysis methods for total mesophilic aerobe bacteria, coliform bacteria, Escherichia coli, Staphylococcus-Micrococcus spp., Yersinia enterocolitica, sulfite reducing anaerobe bacteria, mould&yeast, Salmonella spp. and Listeria monocytogenes. Detection of meat species samples were determined by ELISA. The mean counts of microorganism in the frozen turkey meat which were analyzed, total mesophilic aerobe bacteria were found as 9.5x10 6 cfu/g, coliform bacteria as 2.7x10 4 cfu/g, Escherichia coli as 1.1x10 3 cfu/g, Staphylococcus-Micrococcus spp. as 3.4x10 4 cfu/g, Yersinia enterocolitica as 3.3x10 4 cfu/g, sulfite reducing anaerobe bacteria as 4.5x10 1 cfu/g, mould&yeast as 2.2x10 4 cfu/g. The existence of Salmonella spp, and Listeria monocytogenes in the examined samples were found as 3.63% and 17.27%, respectively. All samples analyzed were found to be turkey meat. In conclusion, this study demonstrates that some pathogens, including Salmonella spp., L. monocytogenes, E. coli and Yersinia enterocolitica are present in frozen turkey meat. Also, the presence of these organisms indicated that there were poor hygienic conditions during the slaughtering, packaging, storage and sales process. Therefore, this food is a serious risk to the public health. Temperature control also is a key issue in producing frozen turkey meat. In addition, it is also important that the products must be manufactured under good hygienic practices.

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“…In another study of 887 food samples, 278 were positive for C. perfringens but only 10 were also cpe-positive [27]. The type A strain of C. perfringens was detected in 22 of 180 turkey meat samples and most were cpe-negative [28]. Most surveys of animal feces and retail foods report low occurrence rates of cpe-positive C. perfringens.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Enterotoxin Gene-positive <i>Clostridium perfringens</i> Spores among Human and Livestock Samples and its Potential as a Human Fecal Source Tracking Indicator

Hashimoto

Tsuchioka

Higashi

et al. 2016

J. of Wat. & Envir. Tech.

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In this study, to evaluate whether Clostridium perfringens could be a useful fecal indicator in aquatic environments and could be employed as a potential source-tracking indicator, the distribution of C. perfringens spores and their toxin types in sewage and livestock fecal samples were analyzed. A total of 804 C. perfringens spore isolates (366 from human-related sewage and effluents, 128 from cattle, 129 from pigs, 72 from chicken, and 109 from abattoir wastewaters) were analyzed using multiplex polymerase chain reaction (PCR) to detect six C. perfringens toxin genes. On the basis of the presence of toxin genes, most of the isolates from both human sewage and livestock samples were determined as C. perfringens type A and they expressed cpa alone or cpa and C. perfringens enterotoxin (cpe) with or without cpb2. Moreover, cpe-positive C. perfringens was detected with frequencies of 29% and 32% in human sewage and effluents, respectively. However, only one isolate (from cattle feces) was cpe-positive among all the livestock samples tested. Thus, the distribution of cpe-positive C. perfringens should be considered an important source tracking indicator for human fecal pollution. Furthermore, we conclude that sewage effluents are a significant source of cpe-positive C. perfringens pollution.

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Molecular typing of Clostridium perfringens isolated from turkey meat by multiplex PCR

Cited by 35 publications

References 21 publications

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

A Study about Microbiological Quality and Species Identification of Frozen Turkey Meat

Distribution of Enterotoxin Gene-positive <i>Clostridium perfringens</i> Spores among Human and Livestock Samples and its Potential as a Human Fecal Source Tracking Indicator

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