Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt

Abstract

IntroductionCanine parvovirus-2 (CPV-2) is one of the most common infectious diseases in dogs characterized by severe gastroenteritis, vomiting, and bloody diarrhea. Little information is available about this topic in Egypt, particularly in the Delta region. This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt.MethodsIn this study, 320 rectal swabs were collected from infected domestic dogs from two districts in delta Egypt. The samples were investigated by rapid immunochromatographic test and polymerase chain reaction for detection the prevalence of CPV-2 variants. The genetic characterization was performed using restriction fragment length polymorphism (RFLP) analysis and partial VP2 gene sequence.Results and discussionThe viral antigen was detected in (264/320, 82.5%) of samples by IC test, while PCR was found more sensitive by detecting (272/320, 85%) positive samples. The RFLP technique using MboII restriction enzyme was successfully used for the differentiation of CPV-2c antigenic variants from CPV-2a/2b strains. Interestingly, the molecular and phylogenetic analysis revealed that both CPV-2a and CPV-2c are circulating in the study area. Deduced amino acid sequence analysis showed changes at residue (N426E) and residue (T440A).: Our results indicated that CPV-2 is prevalent among dogs in Egypt, and therefore further molecular and epidemiological studies of CPV-2 are warranted.

Epidemiological, and molecular investigation of Canine parvovirus-2 infection in Egypt

“…Mekky et al [ 22 ] in Egypt and Ngwa et al [ 32 ] in Cameroon were unclear as to why CPV-2 infections were significantly more prevalent among German shepherd and Pit Bull breeds than in other breeds. Other studies carried out in Egypt reported that Pit Bull [ 33 ], German Shepherd [ 34 ], and Rottweiler [ 22 ] breeds were significantly associated with a higher risk of CPV-2 infections than other breeds of dogs.…”

“…Abdel-Rhman et al [ 42 ], Awad et al [ 43 ], Al-hosary et al [ 21 ], and Etman et al [ 44 ] reported that CPV-2a and CPV-2b are the predominant viruses isolated from infected dogs in Egypt. Zaher et al [ 18 ] and Elbaz et al [ 20 ] previously recorded the CPV-2c genotype in Egypt, while Magouz et al [ 34 ] in a neighboring governorate to the study area indicated that only CPV-2a and CPV-2c were circulating in that area. These latter studies were carried out in restricted geographic regions and time periods, and only partial fragments of the VP2 gene were analyzed.…”