Molecular Typing and Macrolide Resistance of Syphilis Cases in Manitoba, Canada, From 2012 to 2016

Shuel, Michelle; Hayden, Kristy; Kadkhoda, Kamran; Tsang, Raymond S. W.

doi:10.1097/olq.0000000000000734

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…Our findings are in agreement with those reported by Casal et al [ 31 ], i.e., there was an association between PCR and tests for IgM. In addition, a better correlation between PCR and treponemal test vs. non-treponemal VDRL was reported by Shuel et al [ 32 ]. PCR positivity of whole blood samples appears to correlate with the early stages of syphilis.…”

Section: Discussionsupporting

confidence: 93%

“…identification of syphilis. In fact, as shown in this and other studies [31,32], PCR positivity has been found to be positively associated with the results of the treponemal serological test. The group of RPR-negative and treponemal test-positive samples could, at least in part, represent samples from patients with a previous syphilis infection, especially in PCR negative samples taken from patients with an undetermined stage (n = 68).…”

Section: Plos Onesupporting

confidence: 84%

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A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

et al. 2020

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Syphilis, caused by Treponema pallidum ssp. pallidum (TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.

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Section: Discussionsupporting

confidence: 93%

Section: Plos Onesupporting

confidence: 84%

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

et al. 2020

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“…The second most common strains in this study were type 14d/g (also SS14-like clade), which were only found in samples from MSM, and were the second most prevalent type found in MSM samples. Type 14d/g strains were also previously reported to be the dominant strain type in the United States, France, Australia, Canada, and Italy (6,11,12,16,17). A strong link has been reported between type 14d/g strains and macrolide resistance (6,11,12,16,17).…”

Section: Discussionmentioning

confidence: 88%

“…Type 14d/g strains were also previously reported to be the dominant strain type in the United States, France, Australia, Canada, and Italy (6,11,12,16,17). A strong link has been reported between type 14d/g strains and macrolide resistance (6,11,12,16,17).…”

Section: Discussionmentioning

confidence: 88%

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Molecular Typing and Macrolide Resistance Analyses of Treponema pallidum in Heterosexuals and Men Who Have Sex with Men in Japan, 2017

et al. 2019

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In recent years, syphilis notifications have increased dramatically in Japan. We carried out molecular typing and macrolide resistance analyses of Treponema pallidum subsp. pallidum samples collected from patients at four clinics and a hospital in Tokyo and Osaka prefectures in 2017. The macrolide resistant strain type 14d/f (SS14-like clade) was found in significantly more cases of syphilis among heterosexuals than in those among men who have sex with men (MSM); i.e., 79% (31/39) of the strains from heterosexuals were 14d/f compared to 37% (7/19) of those from MSM (odds ratio [OR], 6.6; 95% confidence interval [CI], 1.7 to 26.7; P = 0.002). In addition, 83% (50/60) of the strains were identified as macrolide resistant with an A2058G mutation in the 23S rRNA gene; 90% (35/39) of the strains from heterosexuals were macrolide resistant compared to 58% (11/19) of those from MSM. The odds of having the resistant mutation were considerably higher in the former (OR, 6.4; 95% CI, 1.3 to 33.5; P = 0.02). Heterosexual women and heterosexual men showed similar distributions, and the association remained the same when restricted to men. The strain type distribution and the prevalence of macrolide resistance differed substantially between syphilis strains from heterosexual cases and from MSM cases, suggesting distinct epidemiologic profiles for the two communities and providing important insight into the dynamics of syphilis in Japan.

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Treponema

Shukla

Pereira

Pillay

2022

Molecular Typing in Bacterial Infections, Volume I

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Molecular Typing and Macrolide Resistance of Syphilis Cases in Manitoba, Canada, From 2012 to 2016

Abstract: The finding of an increase in genetic diversity in the strains in this study and an increase in macrolide resistance compared with previous Canadian reports highlighted the need for continued surveillance including strain characterization.

Cited by 8 publications

References 17 publications

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

Molecular Typing and Macrolide Resistance Analyses of Treponema pallidum in Heterosexuals and Men Who Have Sex with Men in Japan, 2017

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