Molecular typing and drug susceptibility of Mycobacterium tuberculosis isolates from Chongqing Municipality, China

Zhang, Dan; An, Jun; Wang, Jianmin; Hu, Chuan; Wang, Zhengui; Zhang, Ruyue; Wang, Yufeng; Pang, Yu

doi:10.1016/j.meegid.2012.10.008

Cited by 26 publications

(37 citation statements)

References 33 publications

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“…M. tuberculosis is divided into 162 clades according to the international spoligotyping database SpolDB4 [26], and the Beijing family is regarded as the most important genetic pattern of the East Asian clade [22]. The pattern is predominant in China, and its proportion is greater in northern China than in southern China.…”

Section: Discussionmentioning

confidence: 99%

“…The genotyping efficiency varied depending on the disparate loci sets in different surveyed areas. To identify a suitable locus set for classifying MTB in Henan, we first chose the 26-locus set to assess the 668 clinical isolates according to previous studies [21, 22, 60]. In total, 567 genotypes, forming 38 clusters, and 529 unique genotypes were obtained by 26-locus VNTR analysis with an HGDI score of 0.9984.…”

Section: Discussionmentioning

confidence: 99%

“…First, each locus was amplified individually as previously described [22]. Then, the PCR products were detected in a 1.5% agarose gel using a 50 bp DNA ladder as the molecular weight standard.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, little was known about the genetic diversity of MTB in this region until now. The study is the first to use26-locus MIRU-VNTR, including the standard 24-locus and two other loci (ETRF and Mtub38) [21, 22]},for assessments in Henan. In this study, we carried out spoligotyping and MIRU-VNTR to classify 668 representative strains from17 cities.…”

Section: Introductionmentioning

confidence: 99%

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Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

et al. 2018

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BackgroundTuberculosis remains a serious threat to human health as an infectious disease in China. Henan, a most populated province in China, has a high incidence of tuberculosis (TB). Though the genetic diversity of Mycobacterium tuberculosis (MTB) has been investigated in many regions, there have been only a few studies on the molecular characteristics and drug resistance phenotypes in Henan. This is the first study on the genetic profile of MTB from Henan.MethodsA total of 668 MTB isolates from various areas were genotyped with spoligotyping and 26-locus MIRU-VNTR (classical 24-locus MIRU-VNTR and 2 other loci). The association between TB spoligotype signatures and drug-resistant profiles was analysed.ResultsOur data revealed that MTB isolates circulating in Henan had a high degree of genetic variation. The Beijing family was the most predominant genotype (83.53%,n = 558), and the typical Beijing type(ST1) was the major sublineage (81.73%,n = 546). In total,668 isolates were divided into 567 different types, forming 38 clusters (2–15 isolates per cluster), and 529 unique types by 26-locus MIRU-VNTR analysis. There was no correlation between the Beijing family and gender, age at diagnosis or treatment history, whereas the Beijing family was significantly associated with all four first-line drug resistance and multidrug-resistant phenotypes. For these samples, 15 of 26 MIRU-VNTR loci had high or moderate discriminatory power according to the Hunter-Gaston discriminatory index. A combination of the 10 most polymorphic loci had similar discriminatory power as the 26-locus set.ConclusionThe Beijing genotype is the most prevalent family. Ten-locus MIRU-VNTR in combination with spoligotyping can efficiently classify the molecular type of MTB in Henan Province.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3351-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

et al. 2018

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“…Although the prevalence of Beijing genotype among most of northern regions was higher than 75%, we also found that Beijing genotype only accounted for 57.3% of MTB isolates in Xinjiang Uygur Autonomous Region [14]. In southern China, the prevalence of Beijing family in Zhejiang (71.6%) was lower than that in Jiangsu (80.5%) [16], Shanghai (79.3%) [17], and Jiangxi (76.7%) [18], while it was higher than that in Chongqing (66.7%) [20], Guangxi (61.9%) [21], Fujian (57.3%) [22], and Guizhou (54.0%) [6–13, 15, 19, 23] (Table 4). …”

Section: Discussionmentioning

confidence: 99%

A First Insight into the Genetic Diversity and Drug Susceptibility Pattern of Mycobacterium tuberculosis Complex in Zhejiang, China

Liu

Chen

et al. 2016

BioMed Research International

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In this study, our aim was to determine the predominant genotypes among the Mycobacterium tuberculosis (MTB) strains circulating in Zhejiang Province. In addition, we also sought to determine the potential associations between MTB genotypes and susceptibility to first-line drugs. Out of these isolates, 673 (71.6%) were classified into the Beijing genotype, while the other 267 (28.4%) were from non-Beijing families. The highest proportion of Beijing genotype was found in Huzhou (80.0%) and the lowest in Lishui (48.3%). Statistical analysis revealed that there was a significant difference in the prevalence of Beijing genotype among different regions (χ 2 = 17.57, P = 0.04). In addition, the overall proportions of drug resistance to INH, RIF, SM, and EMB were 13.2% (124/940), 21.8% (75/940), 3.4% (32/940), and 5.9% (55/940) in Zhejiang, respectively. Further comparison revealed that there was no significant difference in drug susceptibility profiles between Beijing and non-Beijing strains (P > 0.05). In conclusion, we describe the genetic diversity and drug susceptibility pattern of MTB in Zhejiang for the first time. Our data demonstrate that Beijing genotype is the predominant lineage in Zhejiang, while the distribution of Beijing-genotype strains shows geographic diversity. In addition, no correlation is observed between Beijing genotype and anti-TB drug resistance.

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Genetic diversity and drug resistance of Mycobacterium tuberculosis in Yunnan, China

Song

Yang

et al. 2019

Clinical Laboratory Analysis

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BackgroundChina is a country with high burden of tuberculosis (TB), especially drug‐resistant TB (DR‐TB), which is still a serious health problem in Yunnan Province. Mycobacterium tuberculosis (MTB) is the pathogenic microorganism of TB. The epidemiological characteristics of MTB strains in local areas need to be described.MethodsA total of 430 clinical MTB isolates were collected from Yunnan Province and genotyped through the method of 24‐locus mycobacterial interspersed repetitive unit‐variable number tandem DNA repeats (MIRU‐VNTR).ResultsThe genotypes of the 24 loci showed abundantly genetic diversity, and allelic diversity index (h) of these loci varied from 0.012 to 0.817. Among the 430 strains, 30 clusters and 370 unique genotypes were identified. Beijing family was the predominant lineage (70.47%) in Yunnan MTB strains, and the other lineages contained T family (5.81%), MANU2 (0.70%), LAM (3.26%), CAS (0.23%), New‐1 (8.37%), and some unknown clades (11.16%). A total of 74 TB strains were identified as drug resistance through drug susceptibility testing (DST), including 38 multidrug‐resistant TB (MDR‐TB) and 36 single‐drug‐resistant TB (SDR‐TB). The frequency of MDR‐TB strains was significantly higher in Beijing family (10.89%) than that in non‐Beijing family (3.94%, P = 0.032).ConclusionsAlthough MTB strains showed high genetic diversity in Yunnan, China, the Beijing family was still the dominant strain. A high frequency of MDR‐TB strains was recorded in the Beijing family.

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Molecular typing and drug susceptibility of Mycobacterium tuberculosis isolates from Chongqing Municipality, China

Cited by 26 publications

References 33 publications

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China

A First Insight into the Genetic Diversity and Drug Susceptibility Pattern of Mycobacterium tuberculosis Complex in Zhejiang, China

Genetic diversity and drug resistance of Mycobacterium tuberculosis in Yunnan, China

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