Molecular Tuning of IR-786 for Improved Tumor Imaging and Photothermal Therapy

Lim, Wonbong; Byun, Jae Yong; Jo, Gayoung; Kim, Eun Jeong; Park, Min Ho; Hyun, Hoon

doi:10.3390/pharmaceutics14030676

Cited by 14 publications

(6 citation statements)

References 25 publications

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“…In the third cycle, the temperature of the HAP800-PEG solutions mostly returned to the ambient temperature after the continuous laser irradiation. This result revealed that the polymethine cyanine backbone of P800SO3 can be damaged by the concentrated NIR light, which is consistent with previous reports [ 33 , 34 ]. After confirming the optimal accumulation time of the tumor site, we performed the in vivo PTT treatment at 24 h post-injection of HAP800-PEG.…”

Section: Resultssupporting

confidence: 93%

“…According to the previous method [ 35 ], the photothermal conversion efficiency ( η ) of HAP800-PEG was calculated as 34.7%. The η value of HAP800-PEG is similar to that of the polymethine cyanine fluorophores reported previously [ 33 , 34 ]. This result demonstrates that HAP800-PEG can be employed as a promising PTT agent for effective photothermal cancer therapy.…”

Section: Resultssupporting

confidence: 86%

“…The heating and cooling were repeated three times to test the photothermal stability of HAP800-PEG. Based on the equations reported previously [ 33 , 34 , 35 ], the photothermal conversion efficiency ( η ) of HAP800-PEG was calculated as follows:…”

Section: Methodsmentioning

confidence: 99%

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Near-Infrared Fluorescent Hydroxyapatite Nanoparticles for Targeted Photothermal Cancer Therapy

Park

et al. 2023

Pharmaceutics

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Near-infrared (NIR) fluorophores have attracted great attention due to their excellent optical and photothermal properties. Among them, a bone-targeted NIR fluorophore (named P800SO3) contains two phosphonate groups, which play important roles in binding with hydroxyapatite (HAP) as the main mineral component of bones. In this study, biocompatible and NIR fluorescent HAP nanoparticles functionalized with P800SO3 and polyethylene glycol (PEG) were readily prepared for tumor-targeted imaging and photothermal therapy (PTT). The PEGylated HAP nanoparticle (HAP800-PEG) demonstrated improved tumor targetability with high tumor-to-background ratios (TBR). Moreover, the HAP800-PEG also showed excellent photothermal properties, and the temperature of tumor tissue reached 52.3 °C under NIR laser irradiation, which could completely ablate the tumor tissue without recurrence. Therefore, this new type of HAP nanoparticle has great potential as a biocompatible and effective phototheranostic material, which enables the use of P800SO3 for targeted photothermal cancer treatment.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 86%

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Near-Infrared Fluorescent Hydroxyapatite Nanoparticles for Targeted Photothermal Cancer Therapy

Park

et al. 2023

Pharmaceutics

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“…With increasing the concentration from 100 to 300 µM, the temperature changes of the solution reached approximately from 40 to 80 • C upon 808 nm laser irradiation (1.0 W/cm 2 ) in 2 min. As shown in Figure 3d, the photothermal conversion efficiency (η) of IR-783 was calculated to be 28.9%, which is comparable to that of the heptamethine cyanine dyes reported previously [28,29]. This result suggests that the commercial IR-783 can be applied for in vivo photothermal tumor ablation.…”

Section: In Vitro Cytotoxicity Cell Binding and Photothermal Effectsupporting

confidence: 79%

Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

Park,

Hyun

2024

IJMS

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IR-783, a commercially available near-infrared (NIR) heptamethine cyanine dye, has been used for selective tumor imaging in breast, prostate, cervical, and brain cancers in vitro and in vivo. Although the molecular mechanism behind the structure-inherent tumor targeting of IR-783 has not been well-demonstrated, IR-783 has unique properties such as a good water solubility and low cytotoxicity compared with other commercial heptamethine cyanine dyes. The goal of this study is to evaluate the phototherapeutic efficacy of IR-783 as a tumor-targeted photothermal agent in human colorectal cancer xenografts. The results demonstrate that IR-783 shows both the subcellular localization in HT-29 cancer cells and preferential accumulation in HT-29 xenografted tumors 24 h after its intravenous administration. Furthermore, the IR-783 dye reveals the superior capability to convert NIR light into heat energy under 808 nm NIR laser irradiation in vitro and in vivo, thereby inducing cancer cell death. Taken together, these findings suggest that water-soluble anionic IR-783 can be used as a bifunctional phototherapeutic agent for the targeted imaging and photothermal therapy (PTT) of colorectal cancer. Therefore, this work provides a simple and effective approach to develop biocompatible, hydrophilic, and tumor-targetable PTT agents for targeted cancer phototherapy.

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“…24 As different chemical couplings can alter the structure of the targeted ligand, the fluorescence imaging ability, and the therapeutic potential. 25 We successfully synthesized IR-817, a new near-infrared fluorescent dye, by coupling IR-808 with dicyclohexylcarbodiimide in our previous study. 26 The in vitro and in vivo imaging studies revealed that IR-817 functioned as an excellent melanoma-targeting agent.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Near-Infrared Dye IR-817 Encapsulated in Albumin Nanoparticles for Enhanced Imaging and Photothermal Therapy in Melanoma

Wang,

Liao,

Ban

et al. 2023

IJN

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Background: Near-infrared cyanine dyes have high sensitivity and spatial resolution imaging capabilities, but they also have unavoidable drawbacks such as photobleaching, low water solubility, fluorescence quenching, and toxic side effects. As an effective biologic drug carrier, albumin combines with cyanine dyes to form albumin@dye nanoparticles. These nanoparticles can alleviate the aforementioned issues and are widely used in tumor imaging and photothermal therapy. Methods: Herein, a newly synthesized near-infrared dye IR-817 was combined with bovine serum albumin (BSA) to create BSA@IR-817 nanoparticles. Through the detection of fluorescence emission and absorption, the optimal concentration and ratio of BSA and IR-817 were determined. Subsequently, dynamic light scattering (DLS) measurements and scanning electron microscopy (SEM) were used for the physical characterization of the BSA@IR-817 nanoparticles. Finally, in vitro and in vivo experiments were conducted to assess the fluorescence imaging and photothermal therapeutic potential of BSA@IR-817 nanoparticles. Results: IR-817 was adsorbed onto the BSA carrier by covalent conjugation and supramolecular encapsulation, resulting in the formation of dispersed, homogeneous, and stable nanoparticles with a particle size range of 120-220 nm. BSA@IR-817 not only improved the poor water solubility, fluorescence quenching, and toxic side effects of IR-817 but also enhanced the absorption and fluorescence emission peaks in the near-infrared region, as well as the fluorescence in the visible spectrum. In addition, BSA@IR-817 combined with laser 808 irradiation was able to convert light energy into heat energy with temperatures exceeding 50 °C. By creating a mouse model of subcutaneous melanoma, it was discovered that the tumor inhibition rate of BSA@IR-817 was greater than 99% after laser irradiation and that it achieved nearly complete tumor ablation without causing significant toxicity. Conclusion: Our research, therefore, proposes the use of safe and effective photothermal nanoparticles for the imaging, diagnosis, and treatment of melanoma, and offers a promising strategy for future biomedical applications.

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Molecular Tuning of IR-786 for Improved Tumor Imaging and Photothermal Therapy

Cited by 14 publications

References 25 publications

Near-Infrared Fluorescent Hydroxyapatite Nanoparticles for Targeted Photothermal Cancer Therapy

Near-Infrared Fluorescent Hydroxyapatite Nanoparticles for Targeted Photothermal Cancer Therapy

Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

Multifunctional Near-Infrared Dye IR-817 Encapsulated in Albumin Nanoparticles for Enhanced Imaging and Photothermal Therapy in Melanoma

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