Molecular Tools Used in Medical and Veterinary Entomology

Nayduch, Dana; Fryxell, Rebecca Trout; Olafson, Pia U.

doi:10.1016/b978-0-12-814043-7.00028-5

Cited by 4 publications

(2 citation statements)

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“…In addition to morphological identification, molecular methodologies, such as PCR coupled with DNA sequencing, proteomics, and isoenzyme analysis, have been used to identify mosquito species [ 46 , 47 ]. Molecular tools and analyses are especially important to distinguish morphologically similar species.…”

Section: Why Do Mosquito Systematics Matter?mentioning

confidence: 99%

Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control

et al. 2023

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Mosquitoes are vectors of many pathogens, including viruses, protozoans, and helminths, spreading these pathogens to humans as well as to wild and domestic animals. As the identification of species and the biological characterization of mosquito vectors are cornerstones for understanding patterns of disease transmission, and the design of control strategies, we conducted a literature review on the current use of noninvasive and nondestructive techniques for pathogen detection in mosquitoes, highlighting the importance of their taxonomic status and systematics, and some gaps in the knowledge of their vectorial capacity. Here, we summarized the alternative techniques for pathogen detection in mosquitoes based on both laboratory and field studies. Parasite infection and dissemination by mosquitoes can also be obtained via analyses of saliva- and excreta-based techniques or of the whole mosquito body, using a near-infrared spectrometry (NIRS) approach. Further research should be encouraged to seek strategies for detecting target pathogens while preserving mosquito morphology, especially in biodiversity hotspot regions, thus enabling the discovery of cryptic or new species, and the determination of more accurate taxonomic, parasitological, and epidemiological patterns.

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Section: Why Do Mosquito Systematics Matter?mentioning

confidence: 99%

Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control

et al. 2023

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“…Different approaches to mosquito identification are available: morphological, molecular (PCR and nucleic acids sequencing), proteomics tools, isozyme analysis, etc. [ 8 , 9 ]. However, for historical and technical reasons, morphological identification is still the reference method for both research purposes and operational surveillance as it requires little technical equipment, is easy to implement in the field and is inexpensive even when large numbers of individuals need to be identified.…”

Section: Introductionmentioning

confidence: 99%

Identification of mosquitoes (Diptera: Culicidae): an external quality assessment of medical entomology laboratories in the MediLabSecure Network

et al. 2018

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BackgroundIdentification of vectors is of prime importance in the field of medical entomology for both operational and research purposes. An external quality assessment of mosquito identification capacities was carried out within the MediLabSecure Network, which is composed of laboratories located in 19 countries close to the European Union around the Mediterranean and Black seas.MethodsA set of blind samples consisting of 7 or 8 adult mosquitoes and 4 larvae was given to each participant laboratory. In all, 138 adult mosquitoes and 76 larvae of different species were distributed for genus and species identification.ResultsAll identifications were exclusively morphology based. Overall, 81% of identifications were correct at the genus level, 64% at the species level. The results were highly varied among the 19 participating laboratories. The levels of correct identifications were: 100% (three laboratories), 90–95% (four laboratories), 50–75% (six laboratories) and < 50% (six laboratories).ConclusionsThis evaluation showed the need to maintain efforts in capacity building and quality control in the field of medical entomology and, more specifically, in the morphological identification of the Culicidae.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3127-7) contains supplementary material, which is available to authorized users.

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Perspectives in Triatomine Biology Studies: “OMICS”-Based Approaches

Tobias

Latorre-Estivalis

2021

Triatominae - The Biology of Chagas Disease Vectors

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Molecular Tools Used in Medical and Veterinary Entomology

Cited by 4 publications

References 88 publications

Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control

Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control

Identification of mosquitoes (Diptera: Culicidae): an external quality assessment of medical entomology laboratories in the MediLabSecure Network

Perspectives in Triatomine Biology Studies: “OMICS”-Based Approaches

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