Molecular Tools for Bioengineering Eukaryotic Microalgae

Kumar, Anil; Perrine, Zoee; Stroff, Clayton; Postier, Bradley L.; Coury, Daniel A.; Sayre, Richard T.; Allnutt, F. C. Thomas

doi:10.2174/2211550105666160127002147

Cited by 10 publications

(5 citation statements)

References 101 publications

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“…In C. reinhardtii, Chlorella zofingiensis and A. protothecoides, the modified phytoene desaturase (PDS) gene, which protects against herbicides that inhibit carotenoid synthesis, and the modified acetolactate synthase (ALS), conferring resistance against sulfonylurea herbicides, which impairs branch chain amino acid synthesis, have been effectively used for selection of transgenic lines Liu et al 2014;Kumar et al 2016).…”

Section: Transformation and Selection Methodsmentioning

confidence: 99%

“…Microalgae are already susceptible to a variety of abiotic stresses that can be alleviated through single gene inserts. For example, strains overexpressing iron storage proteins (specifically ferritin) enhanced resistance to high illumination, which could be successfully applied as a selection strategy (Kumar et al 2016). Similar strategies for low sulphur, salt or heavy metals could be applied as well.…”

Section: Transformation and Selection Methodsmentioning

confidence: 99%

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Advances and challenges in genetic engineering of microalgae

Fajardo

Donato

Carrasco

et al. 2019

Reviews in Aquaculture

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Knowledge at the molecular level of microalgae has obtained great scientific interest in recent years, because of their potential for biofuel production, as well as other highly valued molecules. Recent advances in the area of the genetic manipulation of microalgae open an entirely new field of possible applications for these organisms as biorefineries. This review represents a compendium that gathers the main milestones that have marked the area of genetic engineering in microalgae during the last decade, from classical techniques for the transformation and expression of transgenes, to the most avant‐garde techniques currently used for gene silencing and DNA edition, as well as a discussion about the future perspectives in this field.

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Section: Transformation and Selection Methodsmentioning

confidence: 99%

Section: Transformation and Selection Methodsmentioning

confidence: 99%

Advances and challenges in genetic engineering of microalgae

Fajardo

Donato

Carrasco

et al. 2019

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…[ 214 ] The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to map the major core transcriptome focusing on the essential metabolic pathways. [ 215 ] For the synthesis of astaxanthin and lipid an industrially important algae C. fingiensis was involved. For enhancing the hydrocarbon production, the metabolic pathway and their enzymes responsible for biosynthesis of terpenoid hydrocarbon in the B. braunii strain Showa was reconstructed, providing a metabolic and genetic framework for alteration.…”

Section: Omics Approaches For Enhancement Of Biorefinery Capabilities...mentioning

confidence: 99%

Integration of Microalgae‐Based Wastewater Bioremediation–Biorefinery Process to Promote Circular Bioeconomy and Sustainability: A Review

Singh

Shourie

Mazahar

2022

CLEAN Soil Air Water

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Bioremediation of wastewater using microalgae is inexpensive, energy efficient, and effective in pollutant reduction as compared to conventional wastewater treatment technologies. Wastewater is a huge resource of minerals, nutrients, bioenergy, and valuable organic compounds and can be used for cultivation of microalgae. The microalgal biomass can be further used as biorefinery feedstock to produce biofuels and commercially important high‐value products. The potential of microalgae toward bioremediation and biorefinery applications presents the avenues for integrating the two processes to support circular bioeconomy and sustainability. This review presents a holistic view of integration of bioremediation and biorefinery processes using microalgae for deriving multiple benefits like pollutant removal, resource recovery, biofuel production, and generation of high‐value commercial products. The current status of high‐throughput microalgal screening technologies is also discussed since the selection of suitable microalgal strains is crucial for the application. The review further summarizes various processes involved in bioremediation and biorefinery systems such as cultivation, bioremediation, harvesting, and downstream processing. Recent trends in microalgal strain improvement for bioremediation and biorefinery applications through genetic engineering, bioinformatics, omics technologies, and genome editing tools are highlighted, while addressing the risks, biosafety issues, and regulatory affairs associated with genetically modified algae.

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“…[18,19]. Another strategy, that does not require the development and selection of mutagenized strains, is the use of genes that provide resistance to abiotic stresses, such as strains overexpressing iron storage proteins [80].…”

Section: Optimization Of Microalgae Applications: Culture Genetics and Proteomicsmentioning

confidence: 99%