Molecular tissue profiling by MALDI imaging: recent progress and applications in cancer research

Lee, Pey Yee; Yeoh, Yeelon; Omar, Nursyazwani; Pung, Yuh Fen; Lim, Lay Cheng; Low, Teck Yew

doi:10.1080/10408363.2021.1942781

Cited by 27 publications

(20 citation statements)

References 133 publications

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“…Here, analysis of so-called "learning curves" in the related domain of micro-array analysis [124,125] have shown promise in determining effective sample sizes [126], and a minimum of 75 specimens per class have been suggested [127]. Here, there are parallels to draw from proteomics using Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) profiling [128][129][130][131], where guidelines for determining the appropriate sample size for clinical proteomic profiling studies have been put forward, using a linear mixed model that allows for the inclusion of estimates of biological and technical replicate variance in a given experiment [132]. In a similar vein, by extending previously published approaches [133,134], the effect of biological variance (e.g., intratumoural heterogeneity), as an important determinant of the overall sample size required, has been examined towards a method that allows for the adjustment of expected biological variance in calculations of sample size [135].…”

Section: Initial Statistical Modeling Should Be Based On Sufficient Sample Numbersmentioning

confidence: 99%

Lipid Profiling in Cancer Diagnosis with Hand-Held Ambient Mass Spectrometry Probes: Addressing the Late-Stage Performance Concerns

et al. 2021

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Untargeted lipid fingerprinting with hand-held ambient mass spectrometry (MS) probes without chromatographic separation has shown promise in the rapid characterization of cancers. As human cancers present significant molecular heterogeneities, careful molecular modeling and data validation strategies are required to minimize late-stage performance variations of these models across a large population. This review utilizes parallels from the pitfalls of conventional protein biomarkers in reaching bedside utility and provides recommendations for robust modeling as well as validation strategies that could enable the next logical steps in large scale assessment of the utility of ambient MS profiling for cancer diagnosis. Six recommendations are provided that range from careful initial determination of clinical added value to moving beyond just statistical associations to validate lipid involvements in disease processes mechanistically. Further guidelines for careful selection of suitable samples to capture expected and unexpected intragroup variance are provided and discussed in the context of demographic heterogeneities in the lipidome, further influenced by lifestyle factors, diet, and potential intersect with cancer lipid pathways probed in ambient mass spectrometry profiling studies.

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Section: Initial Statistical Modeling Should Be Based On Sufficient Sample Numbersmentioning

confidence: 99%

Lipid Profiling in Cancer Diagnosis with Hand-Held Ambient Mass Spectrometry Probes: Addressing the Late-Stage Performance Concerns

et al. 2021

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“…All these feature makes MALDI the ideal technique for routine clinical analysis, as demonstrated by the huge number of existing applications for diagnostic purposes [ 19 , 20 , 21 ]. MALDI has indeed demonstrated a great potential when applied to the fight against cancer [ 22 ], and part of the huge amount of work performed on tumor-related topics, including the analysis of drugs [ 23 ], MALDI-imaging developments [ 24 , 25 ], diagnosis of ovarian [ 26 ], non-small cell lung [ 27 ], and prostate [ 28 ] cancer, has very recently been reviewed.…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF/MS Analysis of Non-Invasive Human Urine and Saliva Samples for the Identification of New Cancer Biomarkers

Zambonin

Aresta

2022

Molecules

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Cancer represents a group of heterogeneous diseases that are a leading global cause of death. Even though mortality has decreased in the past thirty years for different reasons, most patients are still diagnosed at the advanced stage, with limited therapeutic choices and poor outcomes. Moreover, the majority of cancers are detected using invasive painful methods, such as endoscopic biopsy, making the development of non-invasive or minimally invasive methods for the discovery and fast detection of specific biomarkers a crucial need. Among body fluids, a valuable non-invasive alternative to tissue biopsy, the most accessible and least invasive are undoubtedly urine and saliva. They are easily retrievable complex fluids containing a large variety of endogenous compounds that may provide information on the physiological condition of the body. The combined analysis of these fluids with matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF/MS), a reliable and easy-to-use instrumentation that provides information with relatively simple sample pretreatments, could represent the ideal option to rapidly achieve fast early stage diagnosis of tumors and their real-time monitoring. On this basis, the present review summarizes the recently reported applications relevant to the MALDI analysis of human urine and saliva samples.

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“…Coupling data on molecular profiles to advanced data processing algorithms facilitates the development of platforms for various goals. Analysis and comparison of the levels of small biomolecules in the organism can be useful in studies of metabolomics [6], the search for cancer biomarkers [7], disease pathogenesis [8], differentiation between stages [9], reasonable prognosis and the careful selection of the treatment strategy [10].…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Layer of Silver for Detection of Small Biomolecules in Surface-Assisted Laser Desorption Ionization Mass Spectrometry

et al. 2022

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A facile approach for the synthesis of a silver nanostructured layer for application in surface-assisted laser desorption/ionization mass spectrometry of low-molecular-weight biomolecules was developed using electrochemical deposition. The deposition was carried out using the following silver salts: trifluoroacetate, acetate and nitrate, varying the voltage and time. The plate based on trifluoroacetate at 10 V for 15 min showed intense SALDI-MS responses for standards of various classes of compounds: fatty acids, cyclitols, saccharides and lipids at a concentration of 1 nmol/spot, with values of the signal-to-noise ratio ≥50. The values of the limit of detection were 0.71 µM for adonitol, 2.08 µM for glucose and 0.39 µM for palmitic acid per spot. SEM analysis of the plate showed anisotropic flower-like microstructures with nanostructures on their surface. The reduced chemical background in the low-mass region can probably be explained by the absence of stabilizers and reducing agents during the synthesis. The plate synthesized with the developed approach showed potential for future use in the analysis of low-molecular-weight compounds of biological relevance. The absence of the need for the utilization of sophisticated equipment and the synthesis time (10 min) may benefit large-scale applications of the layer for the detection of various types of small biomolecules.

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Molecular tissue profiling by MALDI imaging: recent progress and applications in cancer research

Cited by 27 publications

References 133 publications

Lipid Profiling in Cancer Diagnosis with Hand-Held Ambient Mass Spectrometry Probes: Addressing the Late-Stage Performance Concerns

Lipid Profiling in Cancer Diagnosis with Hand-Held Ambient Mass Spectrometry Probes: Addressing the Late-Stage Performance Concerns

MALDI-TOF/MS Analysis of Non-Invasive Human Urine and Saliva Samples for the Identification of New Cancer Biomarkers

Nanostructured Layer of Silver for Detection of Small Biomolecules in Surface-Assisted Laser Desorption Ionization Mass Spectrometry

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