Molecular testing of gliomas

Abstract

telomerase activity and expression level of hTERT. In the present study, we find out the effect of sodium selenite and selenium l methionine and cadmium chloride on telomerase activity in adenocarcinoma cancer cell line (AGS). Methods: The adenoma carcinoma gastric cancer cells (AGS) were cultured in RPMI1640 supplemented with 10 % fetal bovine serum (FBS) and 1% pen/strep. Concentrations of cadmium chloride, selenium l methionine and sodium selenite were added the adenocarcinoma gastric cancer cells (AGS). Finally, the telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and qRT-TRAP assays. Results: Average telomerase activity (as threshold cycle (CT)) has been detected for three concentrations (5lM and 10lM and 20 lM) of the noted compounds. The results were 24.