Molecular Techniques for Blood and Blood Product Screening

Hu, Yuan

doi:10.1007/978-3-319-95111-9_2

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…The sensitivity of PCR can vary depending on the agent targeted, the sample type, and the timing of specimen collection (early vs. late infection; Brettschneider et al, 1998 ; Brouqui et al, 2004 ). Blood is the most frequent specimen used in molecular testing ( Hu, 2018 ). PCR of whole blood is highly useful for diagnosis of acute anaplasmosis and babesiosis ( Sanchez et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood

et al. 2022

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Assay sensitivity can be a limiting factor in the use of PCR as a tool for the detection of tick-borne pathogens in blood. We evaluated the performance of Tick-borne disease Capture Sequencing Assay (TBDCapSeq), a capture sequencing assay targeting tick-borne agents, to test 158 whole blood specimens obtained from the Lyme Disease Biobank. These included samples from 98 individuals with signs and symptoms of acute Lyme disease, 25 healthy individuals residing in Lyme disease endemic areas, and 35 samples collected from patients admitted to the Massachusetts General Hospital or referred to the infectious disease clinic. Compared to PCR, TBDCapSeq had better sensitivity and could identify infections with a wider range of tick-borne agents. TBDCapSeq identified a higher rate of samples positive for Borrelia burgdorferi (8 vs. 1 by PCR) and Babesia microti (26 vs. 15 by PCR). TBDCapSeq also identified previously unknown infections with Borrelia miyamotoi, Ehrlichia, and Rickettsia species. Overall, TBDCapSeq identified a pathogen in 43 samples vs. 23 using PCR, with four co-infections detected versus zero by PCR. We conclude that capture sequencing enables superior detection of tick-borne agents relative to PCR.

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Section: Introductionmentioning

confidence: 99%

Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood

et al. 2022

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Hyperoncotic human albumin solutions for intravenous fluid therapy: Effectiveness of pathogen safety and purification methods, and clinical safety

Simon¹,

Schumann

Bieri

et al. 2023

Biosafety and Health

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Potential Application of PCR Based Molecular Methods in Fish Pathogen Identification: A Review

Ador

Haque

Paul

et al. 2021

Aquaculture Studies

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Molecular biology developments have led to fast growth in new methods for fish disease diagnosis. Molecular diagnostic methods are rapid and more specific, more sensitive than the culture of pathogens, serology, histology, and biochemical methods which are traditionally utilized to identify causative agent fish disease. Molecular diagnostic methods are valuable for detecting specific pathogens that are difficult to culture in vitro or require a long cultivation period and it significantly more rapid in providing results compared to culture. It enables earlier informed decision-making and rapid diagnosis of bacteremia, particularly for low levels of bacteria in specimens. Molecular techniques which have the major significance are mainly PCR-based molecular diagnostic methods including Polymerase Chain Reaction (PCR), Real-Time Polymerase Chain Reaction (RT-PCR), Multiplex Polymerase Chain Reaction (multiplexPCR), and Random Amplified Polymorphic DNA (RAPD). These have been increasingly utilized to diagnose fish disease for the last recent years. Molecular diagnostic methods can detect pathogens from asymptomatic fish, so disease outbreaks could be prevented. As a consequence, antibiotic treatment can be reduced and the development of antibiotic-resistant bacteria can be eliminated. In this review paper, we attempt to summarize the potentiality of PCR-based molecular diagnostic methods and their application in fish pathogen identification.

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Molecular Techniques for Blood and Blood Product Screening

Cited by 3 publications

References 55 publications

Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood

Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood

Hyperoncotic human albumin solutions for intravenous fluid therapy: Effectiveness of pathogen safety and purification methods, and clinical safety

Potential Application of PCR Based Molecular Methods in Fish Pathogen Identification: A Review

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