Molecular Targets of Herbicides and Fungicides─Are There Useful Overlaps for Fungicide Discovery?

Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance

“…According to studies in the past years, more and more evidence has emerged to support comprehensive metabolism backgrounds underlying fungicide resistance. Such backgrounds include various ERG-encoding genes in fungal ergosterol biosynthesis pathways [36,41,42], acetyl-CoA carboxylase (ACCase)-encoding genes in the lipid and fatty acid oxidation path-ways [45][46][47], reactive oxygen species (ROS)-metabolizing enzyme-encoding genes in the cell wall maintenance and oxidative-stress-responsive processes [50,51], mitochondrial respiratory chain protein-encoding genes in the cellular energy metabolisms [53][54][55], ubiquitin-encoding genes in the post-translational modification processes [57][58][59], and protein kinase-encoding genes involved in mitogen-activated signal transductions [55,60,61]. In the present study, RNA-seq analysis revealed that Pdmfs2 knockout led to the down-regulation of genes involved in peroxisome (ko04146) and oxidative phosphorylation (ko00190) at no prochloraz treatment (Tables 1 and 2, and Figures 1 and 2).…”

“…These metabolisms and cellular stimuli processes in response to specific fungicide(s) have been studied, including ergosterol biosynthesis pathways, lipid and fatty acid oxidation pathways, cell wall maintenance, oxidative-stress-responsive processes, carbohydrate and amino acid metabolisms, cellular energy metabolisms, post-translational modification processes, and signal transduction pathways. All these pathways are highly dependent on many stress-responsive genes, including various ERG-encoding genes (such as erg1, erg3, erg11, erg24, erg25 and so on) [36,[40][41][42], acetyl-CoA carboxylase (ACCase)-encoding genes [43][44][45][46][47], reactive oxygen species (ROS)-metabolizing enzyme-encoding genes [48][49][50][51], mitochondrial respiratory chain protein-encoding genes [52][53][54][55], ubiquitin-encoding genes [56][57][58][59], and a series of protein kinase-encoding genes involved in mitogen-activated signal transductions [55,60,61]. As reported, MFS and ABC transporters played multiple roles in the transport of a diverse range of metabolic substrates and intermediates [62,63].…”

Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC

“…Among the newly published articles, Duke et al explored the overlap of herbicide and fungicide molecular targets . Doherty et al examined worker exposure in residential and golf course environments, and Sun et al shared their cloning, expression, and functional characterization of flavonoid-di- O -glycosyltransferases in maize …”