Molecular targets and anticancer potential of sanguinarine—a benzophenanthridine alkaloid

Galadari, Sehamuddin; Rahman, Anees; Pallichankandy, Siraj; Thayyullathil, Faisal

doi:10.1016/j.phymed.2017.08.006

Cited by 79 publications

(62 citation statements)

References 146 publications

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“…As shown in Figure 3, SC treatment reduced 49.75% of wound closure rate compared to the untreated group, which is consistent with the tube formation assay. Our results are in line with the earlier study where SC exhibited a significantly anti-angiogenic effect in various kinds of tumors (Gaziano et al, 2016;Galadari et al, 2017).…”

Section: Inhibition Of Human Retinal Microvascular Endothelial Cells supporting

confidence: 93%

“…One of the promising candidate is Sanguinarine Chloride (SC) (Mackraj et al, 2008;Basu and Kumar, 2016), a benzo phenanthridine alkaloid derived from the root of Sanguinaria Canadensis and other poppy-fumaria species, which has been proved to exert anti-tumor properties due to its anti-proliferation, antiangiogenic and anti-invasive properties in various preclinical studies (Gaziano et al, 2016;Achkar et al, 2017). Specifically, SC could inhibit angiogenesis of cancer cells via blocking VEGF-induced vessel growth, cell migration, sprouting, and survival (Galadari et al, 2017;Xu et al, 2012;Eun and Koh, 2004;Basini et al, 2007a;Basini et al, 2007c). As the fact that neovascular vessels built in ocular diseases has the similar pathology features with tumor, which are all built to compensate for the lack of oxygen (Hennig and Goepferich, 2015), we hypothesed that SC might also be a suitable antiangiogenic treatment for ocular neovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

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The Antiangiogenic Effect of Sanguinarine Chloride on Experimental Choroidal Neovacularization in Mice via Inhibiting Vascular Endothelial Growth Factor

Zhang

Mao

et al. 2021

Front. Pharmacol.

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Background: The purpose of this study is to investigate the antiangiogenic effect of Sanguinarine chloride (SC) on models of age-related macular degeneration (AMD) both in vivo and in vitro.Methods: Choroidal neovascularization (CNV) was conducted by laser photocoagulation in C57BL6/J mice. SC (2.5 μM, 2 μl/eye) was intravitreally injected immediately after laser injury. The control group received an equal amount of PBS. 7 days after laser injury, CNV severity was evaluated using fundus fluorescein angiography, hematoxylin and eosin (H&E) staining, and choroid flat-mount staining. Vascular endothelial growth factor (VEGF) expression in the retina/choroid complex was measured by western blot analysis and ELISA kit. In vitro, human retinal microvascular endothelial cells (HRMECs) were used to investigate the effects of SC on cell tube formation, migration, and cytotoxicity. The expression of VEGF-induced expression of extracellular signal-regulated kinase (ERK)1/2, protein kinase B (AKT), mitogen-activated protein kinases (p38-MAPK) in vitro and laser induced VEGF expression in vivo were also analyzed.Results: SC (≤2.5 μM) was safe both in vitro and in vivo. Intravitreal injection of SC restrained the formation of laser induced CNV in mice and decreased VEGF expression in the laser site of the retina/choroid complex. In vitro, SC inhibited VEGF-induced tube formation and endothelial cell migration by decreasing the phosphorylation of AKT, ERK1/2, and p38-MAPK in HRMECs.Conclusions: SC could inhibit laser-induced CNV formation via down-regulating VEGF expression and restrain the VEGF-induced tube formation and endothelial migration. Therefore, SC could be a potential candidate for the treatment of wet AMD.

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Section: Inhibition Of Human Retinal Microvascular Endothelial Cells supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The Antiangiogenic Effect of Sanguinarine Chloride on Experimental Choroidal Neovacularization in Mice via Inhibiting Vascular Endothelial Growth Factor

Zhang

Mao

et al. 2021

Front. Pharmacol.

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“…Sanguinarine, a benzophenanthridine alkaloid, exhibits broad‐spectrum anticancer activities, but little attention has been paid to its effects on hypoxia‐induced breast cancer progression in breast cancers.…”

Section: Introductionmentioning

confidence: 99%

Sanguinarine disrupts the colocalization and interaction of HIF‐1α with tyrosine and serine phosphorylated‐STAT3 in breast cancer

Wang

Fan

et al. 2020

J Cellular Molecular Medi

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Breast cancer is one leading cause of death in females, especially triple‐negative breast cancer (TNBC). Hypoxia is a key feature leading to tumour progression driven by hypoxia‐inducible factor (HIF)‐1α. The aim is to investigate the mechanism of HIF‐1α and signal transducer and activator of transcription‐3 (STAT3) interaction and discover a compound to disrupt the interaction in breast cancer cells. The regulation pattern of HIF‐1α and STAT3 was analysed in hypoxic TNBC cells and patient samples. The effects of a natural alkaloid, sanguinarine, on HIF‐1α and STAT3 colocalization and interaction were evaluated in vitro and mouse xenograft models. We observed strong colocalization of HIF‐1α, p‐STAT3‐Tyr and p‐STAT3‐Ser in TNBC patient samples. Sanguinarine could inhibit the nuclear colocalization and interaction of HIF‐1α with p‐STAT3‐Tyr and p‐STAT3‐Ser in vivo and in vitro. Our results may bring insights to the HIF‐1α/STAT3 interaction in breast cancers and suggest sanguinarine as a promising candidate for HIF‐α/STAT3 inhibition.

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“…The most intensively investigated of these is the anticancer activity of sanguinarine and berberine. Many pieces of evidence indicate that sanguinarine inhibits cell cycle and induce apoptosis in various types of cancer cells [19,20]. Sanguinarine induces apoptosis in receptor [21] and mitochondrial pathways [21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines

Och

Zalewski

Komsta

et al. 2019

Toxins

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Isoquinoline alkaloids belong to the toxic secondary metabolites occurring in plants of many families. The high biological activity makes these compounds promising agents for use in medicine, particularly as anticancer drugs. The aim of our study was to evaluate the cytotoxicity and proapoptotic activity of sanguinarine, berberine, and extracts of Chelidonium majus L. and Berberis thunbergii DC. IC10, IC50, and IC90 doses were established toward hematopoietic cancer cell lines using trypan blue staining. Alterations in the expression of 18 apoptosis-related genes in cells exposed to IC10, IC50, and IC90 were evaluated using real-time PCR. Sanguinarine and Chelidonium majus L. extract exhibit significant cytotoxicity against all studied cell lines. Lower cytotoxic activity was demonstrated for berberine. Berberis thunbergii DC. extract had no influence on cell viability. Berberine, sanguinarine, and Chelidonium majus L. extract altered the expression of apoptosis-related genes in all tested cell lines, indicating the induction of apoptosis. The presented study confirmed the substantial cytotoxicity and proapoptotic activity of sanguinarine, berberine, and Chelidonium majus L. extract toward the studied hematopoietic cell lines, which indicates the utility of these substances in anticancer therapy.

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Molecular targets and anticancer potential of sanguinarine—a benzophenanthridine alkaloid

Cited by 79 publications

References 146 publications

The Antiangiogenic Effect of Sanguinarine Chloride on Experimental Choroidal Neovacularization in Mice via Inhibiting Vascular Endothelial Growth Factor

The Antiangiogenic Effect of Sanguinarine Chloride on Experimental Choroidal Neovacularization in Mice via Inhibiting Vascular Endothelial Growth Factor

Sanguinarine disrupts the colocalization and interaction of HIF‐1α with tyrosine and serine phosphorylated‐STAT3 in breast cancer

Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines

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