Molecular symmetry in mitochondrial cardiolipins

Schlame, Michael; Ren, Mindong; Xu, Yang; Greenberg, Miriam L.; Haller, Ivan

doi:10.1016/j.chemphyslip.2005.08.002

Cited by 254 publications

(290 citation statements)

References 38 publications

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“…The relative abundance of liver (18:2) 3 /(18:1) CL, as indicated by the relative abundance of (18:1) fatty acid (m/z 281.23), was about 25 to 30% of that of (18:2) fatty acid (m/z 279.23). These results appear largely in agreement with the study by Schlame et al on CL species distribution in mammalian organs [13].…”

Section: Distribution Of Major CL Species In Rat Organssupporting

confidence: 93%

“…Also, the lack of evidence for the presence of (18:1) [18,19,21]. According to an earlier study, the (18:1) fatty acid chain of rat liver CL was located in sn-2 (or sn-2=) position [13]. In addition, according to earlier studies by Hsu and Turk [18,19], the MS/MS of sodiated CL [MϩNa ϩ -2H] Ϫ preferentially dissociated the fatty acid at sn-2 (or sn-2=) position.…”

Section: Distribution Of Major CL Species In Rat Organsmentioning

confidence: 99%

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Direct MALDI-MS analysis of cardiolipin from rat organs sections

Wang

Jackson

Woods

2007

J. Am. Soc. Mass Spectrom.

108

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Cardiolipins (CL) are mitochondria specific lipids. They play a critical role in ATP synthesis mediated by oxidative phosphorylation. Abnormal CL distribution is associated with several disease states. MALDI-MS and MALDI-MS/MS were used to demonstrate in situ analysis and characterization of CL from tissue sections of organs containing high concentrations of mitochondria. Once the experimental parameters were established, a survey of CL distribution in heart, liver, kidney, leg muscle, and testis was undertaken. The major CL specie in the heart muscle, leg muscle, liver, and kidney is the (18:2) 4 CL, while liver and kidney also contain a minor specie, (18:2) 3 /(18:1) CL. The major CL specie in testis is the (16:0) 4 CL. The CL species distribution in various organs appeared to be in agreement with prior reports. Overall, proper matrix selection, tissue section handling, instrument tuning, and the inclusion of cesium ion in matrix ensured successful in situ MALDI-MS and MALDI-MS/MS analysis of CL. Upon modification and standardization, this method could be streamlined for rapid pathological diagnosis with short turnaround time in clinical settings. (J Am Soc Mass Spectrom 2007, 18, 567-577)

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Section: Distribution Of Major CL Species In Rat Organssupporting

confidence: 93%

Section: Distribution Of Major CL Species In Rat Organsmentioning

confidence: 99%

Direct MALDI-MS analysis of cardiolipin from rat organs sections

Wang

Jackson

Woods

2007

J. Am. Soc. Mass Spectrom.

108

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“…In human heart the predominant C 18 fatty acid is linoleic acid (C 18:2 ) so heart CL contains mostly C 18:2 acyl chains. However human lymphoblast CL contains predominantly oleyl chains (C 18:1 ) [16]. The significance of this specificity is still not understood.…”

Section: Cardiolipin Synthesismentioning

confidence: 99%

Cardiolipin: Setting the beat of apoptosis

2007

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Cardiolipin (CL) is a mitochondria-specific phospholipid which is known to be intimately linked with the mitochondrial bioenergetic machinery. Accumulating evidence now suggests that this unique lipid also has active roles in several of the mitochondria-dependant steps of apoptosis. CL is closely associated with cytochrome c at the outer leaflet of the mitochondrial inner membrane. This interaction makes the process of cytochrome c release from mitochondria more complex than previously assumed, requiring more than pore formation in the mitochondrial outer membrane. While CL peroxidation could be crucial for enabling cytochrome c dissociation from the mitochondrial inner membrane, cytochrome c itself catalyzes CL peroxidation. Moreover, peroxy-CL directly activates the release of cytochrome c and other apoptogenic factors from the mitochondria. CL is also directly involved in mitochondrial outer membrane permeabilization by enabling docking and activation of proapoptotic Bcl-2 proteins. It appears therefore that CL has multiple roles in apoptosis and that CL metabolism contributes to the complexity of the apoptotic process.

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“…This is likely because the organs generally maintain their cardiolipin pattern after development, although cardiolipin remodeling continues during aging with only subtle changes to the acyl chain composition or distribution ( 16,(46)(47)(48).…”

Section: Validation Of Isomeric Cardiolipin Species Determined By Thementioning

confidence: 99%

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

Kiebish

Bell

Yang

et al. 2010

Journal of Lipid Research

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Lipids are critical for the regulation and control of cellular function, membrane dynamics, and signal transduction. The systematic identifi cation and analysis of all cellular lipids in a biological sample is embodied by the rapidly developing fi eld of lipidomics, which has been enabled by recent advances in mass spectrometry ( 1-3 ). A multidimensional MS-based shotgun lipidomics (MDMS-SL) approach for analysis of cellular lipidomes has been demonstrated as one of the most systematic and quantitative high-throughput platforms for global lipidomics capable of analyzing thousands of lipid molecular species directly from the lipid extracts of biological tissues ( 4, 5 ). Analysis of lipidomic data requires extensive use of bioinformatics and computational algorithms for the accurate identifi cation and quantifi cation of lipid molecular species and classes ( 5-9 ). However, most advances in bioinformatics in lipidomics have largely focused on lipid identifi cation rather than interpretation of alterations in biological function resulting in adaptive or pathologic changes in lipid metabolism ( 10 ). Thus, development of bioinformatic and systems biology approaches for the interpretation of lipidomic networks would signifi cantly advance the understanding of the roles of lipids in biological systems ( 3 ).Cardiolipin is a complex polyglycerophospholipid found almost exclusively in mitochondrial membranes of eukaryotic organisms ( 11 ). Cardiolipin is intricately involved in the effi cient production of ATP through utilizing the proton gradient as well as maintaining mitochondrial functionality ( 12-14 ). The diversity of cardiolipin molecular species varies markedly between tissues as well as among Abstract Cardiolipin is a class of mitochondrial specifi c phospholipid, which is intricately involved in mitochondrial functionality. Differences in cardiolipin species exist in a variety of tissues and diseases. It has been demonstrated that the cardiolipin profi le is a key modulator of the functions of many mitochondrial proteins. However, the chemical mechanism(s) leading to normal and/or pathological distribution of cardiolipin species remain elusive. Herein, we describe a novel approach for investigating the molecular mechanism of cardiolipin remodeling through a dynamic simulation. This approach applied data from shotgun lipidomic analyses of the heart, liver, brain, and lung mitochondrial lipidomes to model cardiolipin remodeling, including relative content, regiospecifi city, and isomeric composition of cardiolipin species. Generated cardiolipin profi les were nearly identical to those determined by shotgun lipidomics. Importantly, the simulated isomeric compositions of cardiolipin species were further substantiated through product ion analysis. Finally, unique enzymatic activities involved in cardiolipin remodeling were assessed from the parameters used in the dynamic simulation of cardiolipin profi les. Collectively, we described, verifi ed, and demonstrated a novel approach by integrating both lipidomic ana...

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Molecular symmetry in mitochondrial cardiolipins

Cited by 254 publications

References 38 publications

Direct MALDI-MS analysis of cardiolipin from rat organs sections

Direct MALDI-MS analysis of cardiolipin from rat organs sections

Cardiolipin: Setting the beat of apoptosis

Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics

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