Molecular survey of parvovirus, astrovirus, coronavirus, and calicivirus in symptomatic dogs

Zobba, Rosanna; Visco, S.; Sotgiu, Francesca; Parpaglia, Maria Luisa Pinna; Pittau, Marco; Alberti, Alberto

doi:10.1007/s11259-020-09785-w

Cited by 20 publications

(25 citation statements)

References 86 publications

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“…Initial screening for viral aetiology showed that around 71.6% of gastroenteritis cases were infected with either one or a mixture of the ve tested viruses, whereas none of them tested positive for the presence of CRV (Table S1). Based on a previous molecular survey in symptomatic dogs, viral aetiology in gastroenteritis cases can go up to 93% (Zobba et al 2021). Fig.…”

Section: Prevalence Rate Of Gastroenteritis Causing Virusesmentioning

confidence: 99%

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

Dema

Tallapally

Ganji

et al. 2022

Preprint

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Viral pathogens account for major aetiology of canine gastroenteritis in India. However, till date, there was no structured comprehensive study reported on the viral aetiology of canine gastroenteritis in India. To elucidate this, 475 rectal swabs collected over a period of three years (2018-2021) from clinical canine gastroenteritis cases were screened by real-time PCR for the presence of six majorly known enteric viruses (Canine parvovirus -2 (CPV-2), canine distemper virus (CDV), canine adenovirus-2 (CAdV-2), canine corona virus (CCoV), canine astrovirus (CaAstV), canine rotavirus (CRV)). 71.6% of the samples were found to harbour at least one of the five viruses tested (CPV-2/2a/2b/2c, CDV, CAdV-2, CCoV and CaAstV), whereas, there was no evidence of CRV. The overall incidence rate for each virus was found highest at 64.8% for CPV-2/2a/2b/2c (2a: 21.1%, 2b: 77.4%, 2c: 1.5%), followed by 8% for CDV, 7.2% for CaAstV, 5.9% for CCoV and 4.6% for CAdV-2 with 16.8% incidence for coinfections ranging from two to four viruses in different combinations. Further, for the first time we report the whole genome sequences of CDV, CCoV and CaAstV Indian isolates from canine gastroenteritis cases. Phylogenetic analysis of whole genome revealed that CDV clustered with Asia-1 lineage, CaAstV clustered with group III that consists of China and Brazil isolates, and CCoV clustered with group II that consists of China and Taipei isolates. This study adds to our knowledge on the prevalence of these pathogens in the Indian subcontinent and highlights the need for relevant and routine vaccine prophylactic measures. It is alarming to note that 70.3% of animals vaccinated with DHPPIL were found positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial to address vaccination failures.

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Section: Prevalence Rate Of Gastroenteritis Causing Virusesmentioning

confidence: 99%

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

Dema

Tallapally

Ganji

et al. 2022

Preprint

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“…Os cães são principalmente acometidos por três coronavírus que são eles: dois alfacoronavírus, o CCoV-I e CCoVII e um betacoronavírus CRCoV. Após a descoberta desses, uma recombinação através do vírus da gastroenterite transmissível de suínos e o CCoC-II induziu à diferenciação de dois subtipos, englobando o CCoV-IIa clássico e o CCoV-IIb recombinante (Bonilauri & Rugna, 2021;Zobba et al, 2021). Em geral, as infecções causadas pelo CCoV, são reconhecidas por apresentar alta morbidade, porém baixa mortalidade.…”

Section: Patogenia E Sinais Clínicosunclassified

“…A cepa CCoV-IIa hpervirulenta citada anteriormente, é conhecida como CCoV pantótipo (pCCoV), diagnosticada na Itália 2005, possui a capacidade de dispersão para tecidos extra-intestinais. Por isso, a variante foi descrita como uma enfermidade multissistêmica fatal em cães, com sinais clínicos definidos por leucopenia, gastroenterite, letargia, inapetência, êmese diarreia hemorrágica, ataxia, convulsões e lesões avançadas nos principais órgãos, impactando na resposta imune canina (Alfano et al, 2020;Zobba et al, 2021). Já o betacoronavírus CRCoV provoca sinais clínicos respiratórios leves, por alterar a depuração mucociliar nas vias aéreas superiores (Zappulli et al, 2020).…”

Section: Patogenia E Sinais Clínicosunclassified

Coronavírus canino: Revisão

Sobreira

Costa²

2022

Pubvet

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A presente revisão teve como objetivo evidenciar o coronavírus que atinge os cães, visando recapitular a importância do mesmo em um período de pandemia. O coronavírus canino causa uma infecção altamente contagiosa com sinais clínicos caracterizados como gastroenterites leves a moderadas e respiratórios leves. Sua evolução depende de fatores imunes de cada indivíduo, podendo evoluir para o óbito. O diagnóstico geralmente é difícil, devido as manifestações clínicas que podem ser facilmente confundidos com os de outros patógenos, porém podem-se solicitar exames complementares, tais como observação em microscopia eletrônica, teste ELISA, RT-PCR e testes rápidos em clínicas veterinárias. O tratamento estabelecido é de suporte e variável de acordo com a clínica apresentada. A profilaxia deve ser orientada e inclui vacinação, ingestão de colostro materno, controle ambiental com higienização adequada e isolamento de animais infectados.

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“…It therefore seems that this group 2a "BCoV-like" coronavirus has a very broad host spectrum in wild and farmed mammals and has a high potential for interspecific passage [85] . Otherwise, the comparison of SL-CoV genomes from civets with the human SARS-CoV sequences shows approximately 30 000 nucleotides (a total of 212 positions of variation) of which 209 in a protein coding region (73 of these 209 are silent) [85,86] .…”

Section: Genome Similarity and Conserved Regionsmentioning

confidence: 99%

Human Coronaviruses: Genetics, Virulence Factors and Pathophysiology, Diseases’ Epidemiology

Katawa

Gambogou

Nguepou

et al. 2021

ijirms

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Coronaviruses are pathogens for both animals and humans. The recent Coronavirus Disease (Covid-19), caused by a novel Coronavirus (SARS-CoV-2), outbreak that took place in Hubei (Wuhan, China) from December 2019 aroused numerous questions regarding the nature and the origin of the SARS-CoV-2. Literature review of the main publications from scientific data bases was undertaken. These data revealed similarities of symptoms and identity of genome with the SARS-CoV. In this review, the genetics and virulence factors of coronaviruses, the disease pathophysiology and epidemiology were examined.

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Molecular survey of parvovirus, astrovirus, coronavirus, and calicivirus in symptomatic dogs

Cited by 20 publications

References 86 publications

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

Coronavírus canino: Revisão

Human Coronaviruses: Genetics, Virulence Factors and Pathophysiology, Diseases’ Epidemiology

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