Molecular Survey of Babesia canis in Dogs in Nigeria

Sasaki, Mizuki; Omobowale, Temidayo Olutayo; Tozuka, Morito; Ohta, Kaisaku; Matsuu, Aya; Nottidge, Helen Oyebukola; Hirata, Haruyuki; Ikadai, Hiromi; Oyamada, Takashi

doi:10.1292/jvms.69.1191

Cited by 45 publications

(41 citation statements)

References 12 publications

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“…vogeli isolate in this study clustered in the same subclade as strains found in dogs from the states of São Paulo and Minas Gerais and from countries belonging to different continents, such as Venezuela, Nigeria, South Africa, Egypt and China (Matjila et al 2004, Passos et al 2005, Rey-Valeirón et al 2007, Sasaki et al 2007, Oliveira et al 2009, Xu et al 2015 (Fig.3).…”

Section: Discussionsupporting

confidence: 64%

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

et al. 2017

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Hemoparasitic infections are tick-borne diseases, which affect animals and humans. Considering the importance of canine hemoparasitic infections in veterinary clinics, this study aimed to determine the occurrence of Anaplasma platys, Ehrlichia canis and Babesia vogeli in blood samples from 182 dogs not domiciled in the city of Pato Branco, southwestern region of Paraná State, Brazil, using polymerase chain reaction (PCR). The prevalence of A. platys and B. vogeli was 32.9% and 10.9% respectively, and A. platys infection prevailed (p<0.001). The number of dogs positive for A. platys was larger in Winter (p<0.05). All blood samples were negative for E. canis. In the dogs, infestation by Amblyomma cajennense predominated over that by Rhipicephalus sanguineus (p<0.001); but there was no significant association between PCR and the variables presence of ticks, sex and age. Dogs infected by A. platys and B. vogeli showed thrombocytopenia, lymphopenia and leukocytosis; but there was no correlation between such hematological changes and infection by hemoparasites. This appears to be the first molecular study that demonstrates the existence of A. platys and B. vogeli in dogs from the southwestern region of Paraná.

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Section: Discussionsupporting

confidence: 64%

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

et al. 2017

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“…Our study showed that in Nigeria, 81 of 400 dogs (20.3%) were infected with H. canis, a much lower incidence of infection than in the Sudan. The incidence of B. canis infection in dogs is also lower in Nigeria than in the Sudan [10,11]. R. sanguineus is the dominant tick species in the Sudan [10], and transmits both H. canis and B. canis vogeli.…”

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confidence: 98%

“…In a previous study, we surveyed the prevalence of B. canis infection among 400 dogs in Nigeria using nested PCR and primer sets that amplified the B. canis 18S rRNA gene [11]. B. canis infection resulted in a 698 bp band after nested PCR.…”

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confidence: 99%

“…AY072925, AY072926, L19079) [11]. Forward primer B18S-1 (5'-GGGAGGTAGTGACAAGAAA-3') and reverse primer B18S-2 (5'-TTCCCCGTGTTGAGTCAAA-3') were used for the PCR [6,11].…”

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confidence: 99%

“…The 400 blood samples used for this nested PCR study were identical to those used in our previous study of B. canis infection [11]. Of the 400 dogs, 81 samples produced a 757 bp band, and nine samples produced a 698 bp band.…”

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confidence: 99%

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A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Sasaki

Omobowale

Ohta

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of Hepatozoon canis infections in dogs in Nigeria was surveyed using molecular methods. DNA was extracted from blood samples obtained from 400 dogs. A primer set that amplified the Babesia canis 18S rRNA gene, which has high similarity to the H. canis 18S rRNA gene, was used for the PCR. As a result, samples from 81 dogs (20.3%) produced 757 bp bands, which differed from the 698 bp band that corresponded to B. canis infection. The sequence of the PCR products of 10 samples were determined, all of which corresponded with the H. canis sequence.

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Prevalence and diversity of Hepatozoon canis in naturally infected dogs in Japanese islands and peninsulas

El‐Dakhly¹,

Goto²,

Noishiki³

et al. 2013

Parasitol Res

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Canine hepatozoonosis is a worldwide protozoal disease caused by Hepatozoon canis and Hepatozoon americanum and is transmitted by ixodid ticks, Rhipicephalus and Amblyomma spp., respectively. H. canis infection is widespread in Africa, Europe, South America, and Asia, including Japan. The objective of this study was to study the distribution pattern and diversity of H. canis in naturally infected dogs in nine Japanese islands and peninsulas. Therefore, 196 hunting dogs were randomly sampled during the period from March to September 2011 and the ages and sexes were identified. Direct microscopy using Giemsa-stained blood smears revealed H. canis gametocytes in the peripheral blood of 45 (23.6%) dogs. Polymerase chain reaction (PCR) was performed on EDTA-anticoagulated blood, initially with the common primer set (B18S-F and B18S-R) amplifying the 1,665-bp portion of the 18S rRNA gene, and then with the specific primer set (HepF and HepR) amplifying about 660 bp fragments of the same gene. Based on PCR, 84 (42.9%) dogs were positive using the common primer and 81 (41.3%) were positive using the specific primer. The current investigation indicated that all screened areas, except for Sado Island and Atsumi Peninsula, were infected. Yaku Island had the highest infection rate (84.6% in males and 100.0% in females), while Ishigaki Island showed the lowest infection rates (8.3% in males and 17.7% in females). Both sexes were infected with no significant difference. However, diversity of infection among the surveyed islands and peninsulas was significantly different (P < 0.05). Although H. canis has previously been reported in dogs in Japan, the higher infection rate described in the current study and the diversity of infection in a wide range of islands strongly encourage prospective studies dealing with the prevention and treatment of the infection in dogs, as well as control of ticks.

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Molecular Survey of Babesia canis in Dogs in Nigeria

Cited by 45 publications

References 12 publications

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Prevalence and diversity of Hepatozoon canis in naturally infected dogs in Japanese islands and peninsulas

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