Molecular survey and characterization of Theileria annulata and Ehrlichia ruminantium in cattle from Northwest China

Guo, Huanping; Yin, Chunsheng; Galon, Eloiza May; Du, Jige; Gao, Yang; Moumouni, Paul Franck Adjou; Liu, Mingming; Efstratiou, Artemis; Lee, Seunghun; Li, Jixu; Ringo, Aaron Edmond; Wang, Guanbo; Li, Yongchang; Tumwebaze, Maria Agnes; Xuan, Xuenan

doi:10.1016/j.parint.2018.06.011

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…The only reported Ehrlichia spp. in cattle, E. ruminantium, a causative agent of heartwater (cowdriosis), appears to be restricted to African buffalo in Northern Botswana [28], and to cattle in Mozambique [29], and China [30].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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Background Anaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes. A total of 456 buffalo blood samples from Thailand were investigated. Species identification and genetic differentiation of intra-population and inter-population with the global isolates were conducted based on nucleotide sequences. Interplay between the infection and host factors was also assessed. Results Overall, 41% of water buffalo were found to be infected with rickettsial organisms in the family Anaplasmataceae, but Ehrlichia spp., Neorickettsia spp., and Wolbachia spp. were not found in any of the sequenced samples in this study. Female buffalo were more frequently infected with bacteria in the family Anaplasmataceae than males [71 out of 176 females (40.3%) versus 11 out of 47 males (23.4%)]. The Odds Ratio value indicated that the risk of infection for female buffalo was 2.2-fold higher than that for males (p < 0.05). We detected three haplotypes of A. marginale 16S rRNA gene and they were placed in a clade that was closely related to the A. marginale in buffalo in China; and cattle in Thailand, Uganda, and China. Homology searching of groEL sequences against the GenBank™ database using the BLASTn algorithm revealed that the obtained sequences had a high percentage similarity (98.36–99.62%) to A. platys sequences. The groEL sequences of three A. platys-like isolates were clustered in the same clade as the A. platys from the tick Rhipicephalus microplus in China. Conclusions Our data showed that the apparently healthy buffalo were naturally infected by bacteria in the family Anaplasmataceae at a relatively high prevalence. We also report the finding of A. platys-like infections in water buffalo in Thailand for the first time. Water buffalo serving as the reservoir host of anaplasmosis is of concern for managing the disease control and prevention in ruminants.

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Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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“…Allsopp et al (2015) reported the detection of its genetic material from white-tailed deer in the United States. In China (Guo et al, 2018), Indian Ocean islands (Stachurski et al, 2013), Burkina Faso and Côte d'Ivoire (Biguezoton et al, 2016), Cameroon (Esemu, Ndip, & Ndip, 2018) and South Africa (Mdladla, Dzomba, & Muchadeyi, 2016).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic profiling for zoonoticEhrlichiaspp. from ixodid ticks in the Eastern Cape, South Africa

Adelabu

Iweriebor

Okoh

et al. 2020

Transbound Emerg Dis

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Ticks are obligate hematophagous parasite of vertebrate that transmit a range of pathogenic microorganisms that can cause diseases in livestock and humans. The range of tick-borne disease causative agents infecting domestic animals and humans has recently increased. Several significant zoonotic tick-borne diseases such as ehrlichiosis among others are on the increase worldwide. This study was designed to investigate the occurrence of zoonotic Ehrlichia spp. from samples collected from livestock in selected communities in the Eastern Cape Province, South Africa. Tick samples were manually collected from domesticated animals in selected homesteads.The ticks were morphologically identified to species and tested for Ehrlichia infection via polymerase chain reaction (PCR), using genus-specific disulphide bond formation protein (dsbA) gene primers. This was followed by sequence analysis of amplicons and phylogeny. Of the 1,200 ticks collected, Amblyomma hebraeum was most prevalent (n = 335; 27.9%), followed by Rhipicephalus appendiculatus (n = 274; 22.8%), Rhipicephalus decoloratus; (n = 224; 18.7%) and Rhipicephalus eversti eversti (n = 200, 16.7%). Ehrlichia DNA was detected in 19/1,200 (1.6%) of the screened DNA samples. A homology search of the generated sequences revealed a high percentage of identity between 95% and 98% with other homologous dsbA gene sequences of other Ehrlichia species in GenBank. Phylogenetic analysis showed that the obtained sequences clustered unambiguously with other Ehrlichia sequences from different geographical regions of the world. We concluded that Ehrlichial pathogens are vectored by the ticks collected from domesticated animals in the study areas, thus suggesting concern for public health, as some of the recovered pathogens are zoonotic in nature and could pose serious public health risk through human exposure to tick bites.

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“…Genus Theileria possesses certain unique characteristics which differentiate its species from other apicomplexan parasites e.g., they do not reside in the parasitophorous vacuoles in comparison to Toxoplasma and Plasmodium, are nonmotile and lack apical complex (Bishop et al 2004;von Schubert et al 2010). Theileria species, causing infection in livestock population include: T. annulate, T. parva, T. ovis, T. lestoquardi, T. separate, T. mutans, T. velifera, and T. recondite (Zaeemi et al 2011;Kumsa et al 2013;Li et al 2014;Aydin et al 2015;Guo et al 2018;Remesar et al 2019;Niaz et al 2021;Roy et al 2021). Theileria species are different from Babesia sp.…”

Section: Introductionmentioning

confidence: 99%

Pathobiology of the Tick-Borne Piroplasmosis

2021

Int J Vet Sci

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Molecular survey and characterization of Theileria annulata and Ehrlichia ruminantium in cattle from Northwest China

Cited by 23 publications

References 32 publications

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Phylogenetic profiling for zoonoticEhrlichiaspp. from ixodid ticks in the Eastern Cape, South Africa

Pathobiology of the Tick-Borne Piroplasmosis

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