Molecular surveillance of tuberculosis-causing mycobacteria in wastewater

Mtetwa, Hlengiwe Nombuso; Amoah, Isaac Dennis; Kumari, Sheena; Bux, Faizal; Reddy, Poovendhree

doi:10.1016/j.heliyon.2022.e08910

Cited by 12 publications

(8 citation statements)

References 83 publications

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“…These studies mostly reported the seasonal variation and detection frequency of the ARB targets (Supplementary Tables 1, 5). Two other studies conducted at the single sewershed level used PCRbased methods (Bich et al, 2019;Mtetwa et al, 2021), and one study used shotgun metagenomics (Majeed et al, 2021). Most of these studies compared wastewater-based findings with clinical isolates collected at the single hospital level, while some studies also collected clinical isolates from human patients, and others compared the findings with government reports and secondary literature (Supplementary Table 3).…”

Section: Resultsmentioning

confidence: 99%

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Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review

et al. 2022

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Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help to reduce the emergence and spread of ARB, reduce the associated economic impact, and guide antimicrobial stewardship programs. Wastewater surveillance (WWS) of ARB provides composite samples for a total population, with easy access to the mixed community microbiome. This concept is emerging rapidly, but the clinical utility, sensitivity, and uniformity of WWS of ARB remain poorly understood especially in relation to clinical evidence in sewershed communities. Here, we systematically searched the literature to identify studies that have compared findings from WWS of ARB and antibiotic resistance genes (ARG) with clinical evidence in parallel, thereby evaluating how likely WWS of ARB and ARG can relate to the clinical cases in communities. Initially, 2,235 articles were obtained using the primary search keywords, and 1,219 articles remained after de-duplication. Among these, 35 articles fulfilled the search criteria, and an additional 13 relevant articles were included by searching references in the primary literature. Among the 48 included papers, 34 studies used a culture-based method, followed by 11 metagenomics, and three PCR-based methods. A total of 28 out of 48 included studies were conducted at the single sewershed level, eight studies involved several countries, seven studies were conducted at national or regional scales, and five at hospital levels. Our review revealed that the performance of WWS of ARB pathogens has been evaluated more frequently for Escherichia coli, Enterococcus spp., and other members of the family Enterobacteriaceae, but has not been uniformly tested for all ARB pathogens. Many wastewater-based ARB studies comparing the findings with clinical evidence were conducted to evaluate the public health risk but not to relate with clinical evidence and to evaluate the performance of WWS of ARB. Indeed, relating WWS of ARB with clinical evidence in a sewershed is not straightforward, as the source of ARB in wastewater cannot be only from symptomatic human individuals but can also be from asymptomatic carriers as well as from animal sources. Further, the varying fates of each bacterial species and ARG within the sewerage make the aim of connecting WWS of ARB with clinical evidence more complicated. Therefore, future studies evaluating the performance of many AMR pathogens and their genes for WWS one by one can make the process simpler and the interpretation of results easier.

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Section: Resultsmentioning

confidence: 99%

“…Relatively few studies (only three) have reported PCR-based approaches with qPCR (Bich et al, 2019), high-throughput qPCR (Pärnänen et al, 2019), or dPCR (Mtetwa et al, 2021). These approaches can overcome some limitations of culturebased methods and can detect microbes that are difficult to culture (Tiwari et al, 2022b).…”

Section: Monitoring Methodsmentioning

confidence: 99%

Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review

et al. 2022

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“…Total mycobacteria and MTBC count varied per plant in untreated wastewater samples. In addition , M. bovis and M. caprae, two other MTBC organisms, were also detected [ 47 ].…”

Section: Presence Of Mtbc Organisms In the Environmentmentioning

confidence: 99%

Bovine tuberculosis in India: The need for One Health approach and the way forward

Ramanujam

Palaniyandi

2023

One Health

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“…In addition, the Mycobacterium spp. seems resistant in pastures and in water environments [ 12 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Estimated Prevalence of Tuberculosis in Ruminants from Slaughterhouses in Constantine Province (Northeastern Algeria): A 10-Year Retrospective Survey (2011–2020)

Dergal

Ghermi

Imre

et al. 2023

Life

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Tuberculosis (TB) is considered one of the most widespread and devastating zoonotic diseases in low-income countries, with a cosmopolitan distribution. The aim of this 10-year retrospective survey (from 2011 to 2020) was to determine the frequency of bovine, ovine, and goat tuberculosis in different local slaughterhouses across Constantine Province, Algeria. The control of livestock carcasses was systematically performed by veterinarian inspectors, after each stage of the slaughter process. The routine abattoir inspection included the detection of visible abnormalities on different organs and lymph nodes. The overall prevalence of tuberculosis recorded in slaughtered animals was 0.83%, with the following distribution among species: 2.73% in cattle, 0.001% in sheep, and 0.0% in goats. During the study period, there was a strong correlation (R = 0.82) (p < 0.01) between tuberculosis occurrence and the number of slaughtered cattle. Fluctuations in monthly TB prevalence ranged from 2% to 24.8% between 2018 and 2020, although there were no statistically significant correlations between infection and the age or gender of the animals, except for the year 2020 when a significantly higher (p = 0.017) percentage of TB cases were recorded in female cattle compared to male cattle. The average monthly weight of the confiscated livers and lungs ranged significantly (p ≤ 0.05) from 150 kg to 350 kg. The study results provide baseline data regarding livestock tuberculosis monitoring in the area of Constantine, Algeria, indicating that the disease incidence is not highly alarming, yet remains a serious public and animal health issue in the screened region.

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Molecular surveillance of tuberculosis-causing mycobacteria in wastewater

Cited by 12 publications

References 83 publications

Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review

Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review

Bovine tuberculosis in India: The need for One Health approach and the way forward

Estimated Prevalence of Tuberculosis in Ruminants from Slaughterhouses in Constantine Province (Northeastern Algeria): A 10-Year Retrospective Survey (2011–2020)

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