Molecular study on the effect of dandelion leaf extract and silver nanoparticles in induced diabetic albino rats

Abstract

The present study investigated the antidiabetic effects of dandelion leaf extract and silver nanoparticles on diabetic albino rats at molecular level, and compared with insulin. Silver nanoparticles were prepared by green chimestry method using dandelion leaf extract as capping and reducing agent, which reduced Silver nitrate (AgNO3) into silver nanoparticles. The morphology, size and crystalline structure, of silver nanoparticles are confirmed by ultraviolet, Scanning electron microscope technique, and X-ray diffraction respectively. The formation and stability of the reduced AgNPs in colloidal solution were detected and monitored by using UV-visible spectroscopy, and the absorption peak appear at 450 nm indicating the specific surface Plasmon resonance of AgNPs. The Scanning Electron Microscope showed that the AgNPs have spherical shape with aparticular size ranging from (13 to 28 )nm.The chemical characterization of synthesized AgNPs was tested using the X-ray diffraction technique, which shows that the average crystalline size was 16nm, and the synthesized silver nanoparticles have a polycrystalline structure. Application of nanoparticles in medicine is an attractive proposition. The present study included 35 female wistar albino rats weighting 200±20 grams.