Molecular study of the presence and transcriptional activity of HPV in semen

Faja, Fabiana; Pallotti, Francesco; Bianchini, Serena; Buonacquisto, Alessandra; Cicolani, Gaia; Conflitti, Anna Chiara; Fracella, Matteo; Cavallari, Eugenio Nelson; Sciarra, Francesca; Pierangeli, Alessandra; Paoli, Donatella; Lenzi, Andrea; Antonelli, Guido; Lombardo, Francesco; Gianfrilli, Daniele

doi:10.1007/s40618-023-02167-4

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…Therefore, HPV infection of semen may be responsible for the reduction in natural and assisted pregnancy rates and a risk factor for pregnancy interruption ( 31 ). The exact mechanism by which sperm infection impairs fertility requires further investigation ( 22 , 30 ), considering that the transcriptional activity of HPV has never been detected in human semen ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen

Cutarelli,

De Falco,

Brunetti

et al. 2024

Front. Vet. Sci.

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Virological evaluation was performed on equine semen to detect the presence of papillomaviruses (PVs) using droplet digital polymerase chain reaction (ddPCR) as the aim of this study was to investigate whether the sperm from asymptomatic stallions harbors ovine papillomaviruses (OaPVs). Twenty-seven semen samples were analyzed, 18 of which were commercially acquired. The remaining nine samples comprising semen and peripheral blood, were collected from nine stallions with no apparent signs of PV-related diseases during clinical examination at the Didactic Veterinary University Hospital (DVUH) of Naples. OaPV was detected in 26 semen samples. OaPV1 was the most prevalent virus infecting equine semen. OaPV1 infected 21 semen samples (~80.8%) and showed a high number of DNA and RNA copies per microliter. qPCR was used to detect OaPV1 DNA in the 18 semen samples. ddPCR was used to detect and quantify the expression of OaPV2, OaPV3, and OaPV4. qPCR failed to detect DNA for these genotypes. Additionally, ddPCR was used to detect the transcriptionally active OaPV1 in six blood and semen samples from the same stallion. ddPCR failed to detect any nucleic acids in OaPVs in peripheral blood samples from the three stallions. In one semen sample, ddPCR detected OaPV1 DNA but failed to detect any nucleic acid in the remaining two semen samples, and peripheral blood from the same animals of the remaining 18 semen samples was not available, OaPV1 and OaPV4 were responsible for nine and five single infections, respectively. No single infections with either OaPV3 or OaPV4 were seen.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen

Cutarelli,

De Falco,

Brunetti

et al. 2024

Front. Vet. Sci.

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Papillomavirus infection and male infertility: A systematic review and meta‐analysis

Garolla,

Mereu,

Pizzol

et al. 2024

Health Science Reports

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Background and AimsIncreasing attention is being paid to the role of human papillomavirus (HPV) in men and specifically reproduction. Growing evidence suggests an association between HPV infection with many adverse effects including the impairment of semen parameters, the increase of blastocyst apoptosis, the reduction of endometrial implantation of trophoblastic cells, as well as the increase rate of miscarriages and spontaneous preterm birth.MethodsWe systematically searched PubMed/MEDLINE, Scopus, Embase, Web of Science, CINHAL, PsycINFO, and ERIC from inception to 2nd of July 2024, for studies that investigated the association between HPV infection with sperm parameters and fertility outcomes. The meta‐analysis was conducted on mean data and standard deviations.ResultsWe included 25 studies with a total of 6942 patients. Sperm morphology was lower in HPV positive groups versus HPV negative control groups (SMD = ‐0.52 95% CI −0.84; −0.21; p = 0.001). Sperm motility was also significantly lower in HPV positive groups when compared to HPV negative controls (SMD = −0.82 95% CI −1.07; −0.57; p = <0.001). Sperm volume, concentration, and pH were not significantly different between the two groups. The other 15 studies included in the systematic review for which it was not possible to conduct a meta‐analysis showed strong associations between HPV infection and impairment of sperm parameters, reduced couple fertility and increased risk of pregnancy loss.ConclusionsThe current evidence highlights the link between HPV infection and sperm parameters, male fertility and reproductive outcomes, which has the potential to lead to a decreased couple fertility, increased risk of pregnancy loss, re‐infection and increased treatment costs.

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Update on known and emergent viruses affecting human male genital tract and fertility

Dabizzi,

Maggi,

Torcia

2024

Basic Clin. Androl.

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Many viruses infect the male genital tract with harmful consequences at individual and population levels. In fact, viral infections may induce damage to different organs of the male genital tract (MGT), therefore compromising male fertility. The oxidative stress, induced during viral-mediated local and systemic inflammation, is responsible for testicular damage, compromising germinal and endocrine cell functions. A reduction in sperm count, motility, number of normal sperm and an increase in DNA fragmentation are all common findings in the course of viral infections that, however, generally regress after infection clearance. In some cases, however, viral shedding persists for a long time leading to unexpected sexual transmission, even after the disappearance of the viral load from the blood.The recent outbreak of Zika and Ebola Virus evidenced how the MGT could represent a reservoir of dangerous emergent viruses and how new modalities of surveillance of survivors are strongly needed to limit viral transmission among the general population.Here we reviewed the evidence concerning the presence of relevant viruses, including emergent and re-emergent, on the male genital tract, their route of entry, their adverse effects on male fertility and the pattern of viral shedding in the semen.We also described laboratory strategies to reduce the risk of horizontal or vertical cross-infection in serodiscordant couples undergoing assisted reproductive technologies.

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Molecular study of the presence and transcriptional activity of HPV in semen

Cited by 4 publications

References 52 publications

Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen

Molecular detection of transcriptionally active ovine papillomaviruses in commercial equine semen

Papillomavirus infection and male infertility: A systematic review and meta‐analysis

Update on known and emergent viruses affecting human male genital tract and fertility

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