Molecular study of quorum sensing and biofilm formation genes in pseudomonas aeruginosa isolated from UTIs patients

Abstract

Background: Urinary tract infection (UTI) is a common health problem in both community and nosocomial settings, affecting both men and women equally. Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Biofilm is an architecture built mostly by autogenic extracellular polymeric substances which function as a scaffold to encase the bacteria together on surfaces, and to protect them from environmental stresses, impedes phagocytosis and thereby conferring the capacity for colonization and long-term persistence. So, the aim of this study to screen of some important quorum sensing and biofilm genes among pseudomonas aeruginosa isolated from UTIs patients. Methods: These study was conducted in Al-Qadisiyah province, Iraq at five major hospitals (AL-Diwanyia Teaching Hospital, Feminine and children teaching hospital, Afak General Hospital, AL-Hamzah General Hospital and AL-Shamiya General Hospital) during the period from (November, 2020 to June, 2021). A total of 800 urine samples were collected from male and females referring to five major hospitals. The age of the patients ranged from (1 to 80) years-old.

Study of pyocyanin production and biofilm formation in clinical Pseudomonas aeruginosa

“…shown Figure (2,3). Biofilm formation was studied [30] on local isolates of P. aeruginosa and found that 45% were strongly biofilm-forming, 48% moderate, and 6.25% were non-biofilm-producing which agreed with current study. However, the percentages obtained in this study differ from [34] which was 100% strong biofilm-producing isolates.…”