Molecular studies resolve Nyholmiella (Orthotrichaceae) as a separate genus

Sawicki, Jakub; Plášek, Vítězslav; Szczecińska, Monika

doi:10.1111/j.1759-6831.2010.00076.x

Cited by 46 publications

(44 citation statements)

References 35 publications

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“…One of the largest genera within the family, Orthotrichum, composed of at least 162 species [31], has been updated according to a number of sources [10,[32][33][34][35][36][37][38][39][40]. Conversely, the genus Nyholmiella includes only two taxa (Nyholmiella gymnostoma and N. obtusifolia) [41]. Most are epiphytic mosses, occasionally growing as epiliths [42].…”

Section: Studied Speciesmentioning

confidence: 99%

Modeling the distribution of rare and interesting moss species of the family Orthotrichaceae (Bryophyta) in Tajikistan and Kyrgyzstan

2017

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Bryological research carried out from 2008 in Tajikistan and Kyrgyzstan brought interesting data on the occurrence of epiphytic bryophytes which have not been recorded yet there. One of the species was recently described as a new (Orthotrichum pamiricum) and some of the other newly recorded species are considered as rare or endangered in the region of Middle Asia. To make detailed field monitoring of the species with the aim of mapping their distribution in a wild and complex mountainous terrain, it was necessary in the first instance to identify the area with suitable conditions for the occurrence of these species. We present an innovative modeling program MaxEnt (maximum entropy modeling), which have not previously been used for modeling either epiphytic bryophytes or in the Middle Asia region. Using 205 samples (presence-only data), percent tree cover, and seven uncorrelated bioclimatic variables, regions suitable for the occurrence of the studied species were identified. Distribution models for eight most interesting species of Orthotrichum are presented here (O. affine, O. anomalum, O. crenulatum, O. cupulatum, O. pallens, O. pamiricum, O. pumilum, and O. speciosum). They indicated appropriate areas for the most probable occurrence of the species in western Tajikistan, and southwestern and northeastern Kyrgyzstan. These results could serve as guides for future survey expeditions, and aid in the conservation of target species and our understanding of their ecology. Different environmental variables for various species were selected as the most important for modeling. However, for most species higher minimum temperatures and higher precipitation in the wettest month and mean diurnal range were the variables with the greatest contribution to the models.

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Section: Studied Speciesmentioning

confidence: 99%

Modeling the distribution of rare and interesting moss species of the family Orthotrichaceae (Bryophyta) in Tajikistan and Kyrgyzstan

2017

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“…Extracted DNA samples were stored at -20°C. For amplification and sequencing of ITS we used the primers of Fiedorow et al [8], which were successfully used in previous studies on genus Orthotrichum [4,[9][10][11][12]. The ITS were amplified in a volume of 25 μl containing 20 mM (NH 4 ) SO 4 , 50 mM Tris-HCl (pH 9.0 at 25°C), 1.5 mM MgCl 2 , 1 μl BSA, 200 μM each dATP, dGTP, dCTP, dTTP, 1.0 μM of each primer, one unit of Taq polymerase (Novazym) and 1 μl of the DNA solution.…”

Section: Molecular Analysismentioning

confidence: 99%

Orthotrichum affine var. bohemicum (Orthotrichaceae), a new variety of epiphytic moss from the Czech Republic

et al. 2011

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Orthotrichum affine var. bohemicum Plášek & Sawicki, var. nova, is a confirmed record of a new variety from the Czech Republic. The new taxon is described and illustrated by photos and drawing. It is characterized by having superficial stomata and a peristome with 16 endostome segments (practically as long as exostome teeth). Molecular data indicating the phylogenetic placement of the species are presented

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“…They are characterized by high efficiency and a relatively low cost of use [34]. These markers are widely used in both taxonomic and population genetics studies of bryophytes [15,[35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

“…ISJ primers are partly complementary to the sequences on the exon-intron boundary. At first these primers were used in studies of vascular plants [20,40,41], but they also proved to be a useful tool in the taxonomy of bryophytes [21,37,38]. In addition to the above marker classes, sequences complementary to the katG gene coding the enzyme catalaseperoxidase were also used.…”

Section: Introductionmentioning

confidence: 99%

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Sawicki

Szczecińska

2011

Acta Soc Bot Pol

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IntroductionDue to their great phenotypic plasticity, the classification of peat moss species often requires microscopic identification, which is both labor-and time-consuming. In addition to a standard morphological description, the classification of bryophytes increasingly often involves the use of molecular markers [1,2]. Chloroplast and nuclear genomes are mainly used, but this approach implies high analysis costs. Species-specific molecular markers support material identification even when diagnostic traits cannot be observed. The above applies mostly to traits related to sexual reproduction (gametengia, sporophytes) and vegetative reproduction (gemmae) which are often undeveloped or already degraded.The identification of peat moss species possess some problems. According to various authors, their number ranges from 39 [3] to 340 [4]. The main reason for such a wide variation in the reported number of Sphagnum species is their high phenotypic plasticity. Mosses growing in wet habitats exhibit high morphological variability in response to water level fluctuations and changes in other environmental conditions [5]. Another factor that affects morphological variability and hinders the species classification of peat mosses is hybridization [6][7][8][9].Isoenzymatic electrophoresis is a rapid and relatively inexpensive method of molecular species identification. The use of markers of this class permitted the molecular identification of the majority of morphologically controversial species of the genus Sphagnum [10,11]. However, their practical application is difficult, as in order to obtain stable and distinct isoenzymatic patterns, plants should be grown under glass-house conditions to normalize their expression levels and increase vitality [12]. Electrophoresis of enzymatic proteins cannot be applied to herbaceous materials, either, which makes it useless as a tool for verifying the correctness of species identification. Therefore, other molecular markers enabling a rapid, reliable and inexpensive identification have to be found.PCR-based DNA analyses provided new insights into the taxonomy of peat mosses. Studies of the genus Sphagnum rely primarily on RAPD [13,14], ISSR [9,15] and SSR [16] markers, and ITS sequences [17,18]. With a few exceptions [19], the above markers have not been used for molecular species identification.The objective of the present study was to evaluate the suitability of five types of DNA markers for molecular species identification. Peat moss species of the section Acutifolia were analyzed. Apart from markers that are commonly applied in studies of the genus Sphagnum, i.e. RAPD, ISSR and nuclear ITS regions, we also tested ISJ markers and primers complementary to the katG gene, which had been successfully used as species-specific markers in higher plants [20] The nuclear ITS region, analyzed based on the polymorphism of restriction loci [24,25] and a direct comparison of sequences [18,26], is widely used in the taxonomic studies of bryophytes. An extensive review of ITS applications i...

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Molecular studies resolve Nyholmiella (Orthotrichaceae) as a separate genus

Cited by 46 publications

References 35 publications

Modeling the distribution of rare and interesting moss species of the family Orthotrichaceae (Bryophyta) in Tajikistan and Kyrgyzstan

Modeling the distribution of rare and interesting moss species of the family Orthotrichaceae (Bryophyta) in Tajikistan and Kyrgyzstan

Orthotrichum affine var. bohemicum (Orthotrichaceae), a new variety of epiphytic moss from the Czech Republic

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

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