Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.

Minghetti, Phillip P.; Ruffner, Duane E.; Kuang, Wun-Jing; Dennison, Olivia; Hawkins, James W.; Beattie, Wanda G.; Dugaiczyk, Achilles

doi:10.1016/s0021-9258(19)62680-3

Cited by 280 publications

(39 citation statements)

References 68 publications

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“…These values are very close to those of human and bovine a-fetoprotein (Luft & Lorscheiser 1983). Its primary sequence was known before that derived from complementary DNA (cDNA) data (Minghetti et al 1986) and shows that HSA is a single, 66 kDa monomeric polypeptide of 585 amino acid residues, stabilized by 17 disulphide bridges (Dugaiczyk et al 1982;Sugio et al 1999), whose locations were firstly derived from peptide studies by Brown (1974) and Saber et al (1977). HSA shows a peculiar amino acid distribution, compared with that of a typical protein, with a low content of Met, Gly and Ile, while the ionic residues (Glu and Lys) are very abundant.…”

Section: Introductionsupporting

confidence: 68%

Adsorption of human serum albumin on the chrysotile surface: a molecular dynamics and spectroscopic investigation

Artali

Prà

Foresti

et al. 2007

J. R. Soc. Interface.

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The human serum albumin (HSA) secondary structure modifications induced by the chrysotile surface have been investigated via computational molecular dynamics (MD) and experimental infrared spectroscopy (FTIR) on synthetic chrysotile nanocrystals coated with different amount of HSA. MD simulations, conducted by placing various albumin subdomains close to the fixed chrysotile surface, show an initial adsorption phase, accompanied by local rearrangements of the albumin motifs in contact with the chrysotile layer. Next, large-scale rearrangements follow with consequent secondary structure modifications.Gaussian curve fitting of the FTIR spectra obtained for HSA-coated synthetic chrysotile nanocrystals has allowed the quantification of HSA structural modifications as a function of the amount of protein adsorbed. The experimental results support the atomistic computer simulations providing a realistic description of the adsorption of plasma proteins onto chrysotile and unravelling a key step in the understanding of asbestos toxicity.

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Section: Introductionsupporting

confidence: 68%

Adsorption of human serum albumin on the chrysotile surface: a molecular dynamics and spectroscopic investigation

Artali

Prà

Foresti

et al. 2007

J. R. Soc. Interface.

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“…Serum proteins, such as albumin, are the ideal delivery templates of choice by many scientific groups. Due to their natural occurrence, availability of many reactive groups for glycan clustering, in vivo stability, and negligible immunogenicity in clinical applications, synthetic glycoalbumins should experience little biological interference in the blood [24].…”

Section: Introductionmentioning

confidence: 99%

Homo- and Heterogeneous Glycoconjugates on the Basis of N-Glycans and Human Serum Albumin: Synthesis and Biological Evaluation

et al. 2022

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Neoglycoconjugates mimicking natural compounds and possessing a variety of biological functions are very successful tools for researchers to understand the general mechanisms of many biological processes in living organisms. These substances are characterized by high biotolerance and specificity, with low toxicity. Due to the difficult isolation of individual glycoclusters from biological objects, special interest has been directed toward synthetic analogs. This review is mainly focused on the one-pot, double-click methodology (containing alkyne–azide click cycloaddition with the following 6π-azaelectrocyclization reactions) used in the synthesis of N-glycoconjugates. Homogeneous (including one type of biantennary N-glycan fragments) and heterogeneous (containing two to four types of biantennary N-glycan fragments) glycoclusters on albumin were synthesized via this strategy. A series of cell-, tissue- and animal-based experiments proved glycoclusters to be a very promising class of targeted delivery systems. Depending on the oligosaccharide units combined in the cluster, their amount, and arrangement relative to one another, conjugates can recognize various cells, including cancer cells, with high selectivity. These results open new perspectives for affected tissue visualization and treatment.

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“…Patients are paucisymptomatic exept for fatigue, minimal ankle oedema and hypotension. CA is a Mendelian recessive trait caused by a variety of mutations in the gene located on chromosome 4 q13.3, coding for albumin 4,5 . Only 78 cases were reported, and the estimated disease prevalence is less than one in a million.…”

Section: Introductionmentioning

confidence: 99%

“…CA is a Mendelian recessive trait caused by a variety of mutations in the gene located on chromosome 4 q13.3, coding for albumin. 4,5 Only 78 cases were reported, and the estimated disease prevalence is less than one in a million.Albumin is the most important protein contributing to the regulation of plasma osmotic pressure. Individuals with analbuminemia have elevated levels of low-density lipoproteins and other plasma proteins, including coagulation factors, as a compensatory mechanism.…”

mentioning

confidence: 99%

Oxidative stress mediated platelet activation in patients with congenital analbuminemia: Effect of albumin infusion

Baratta

Bartimoccia

Carnevale

et al. 2021

Journal of Thrombosis and Haemostasis

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Background and Aims Congenital analbuminemia is a rare autosomal recessive inherited disorder characterized by strongly decreased concentration, or complete absence, of serum albumin (SA). Several lines of evidence indicate that SA has anti‐thrombotic effect. In vivo platelet function and the role of oxidative stress (OS) in platelet aggregation promotion have never been studied in analbuminaemic patients. Patients and Methods We report two cases of congenital analbuminemia in a 38‐year‐old male and in a 67‐year‐old woman. We analyzed platelet activation (PA) and OS at baseline and 2 h after 40 g human albumin infusion. PA was evaluated as platelet aggregation, sCD40L and surface αIIbβ3 integrin and P‐selectin expression. OS was evaluated measuring serum sNOX2dp, and 8‐iso‐PGF2α. Findings Analbuminemic patients displayed higher platelet aggregation, markers of PA and of OS. Albumin infusion reduced platelet activation by reducing oxidative stress.

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Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.

Cited by 280 publications

References 68 publications

Adsorption of human serum albumin on the chrysotile surface: a molecular dynamics and spectroscopic investigation

Adsorption of human serum albumin on the chrysotile surface: a molecular dynamics and spectroscopic investigation

Homo- and Heterogeneous Glycoconjugates on the Basis of N-Glycans and Human Serum Albumin: Synthesis and Biological Evaluation

Oxidative stress mediated platelet activation in patients with congenital analbuminemia: Effect of albumin infusion

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