Molecular structure and genetic mapping of the mouse gastrin gene

Friis‐Hansen, Lennart; Rourke, Ian J.; Bundgaard, Jens R.; Rehfeld, Jens F.; Samuelson, Linda C.

doi:10.1016/0014-5793(96)00430-9

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Gastrin has been reported to be expressed not only in the antrum but other organs such as the duodenum, pancreas, brain, pituitary glands, testis, and ovary in variety of species (11,12,27,29). In this study, we detected weak GFP signals in the anterior lobe of the pituitary gland, but not in the other organs such as duodenum, pancreas, testis, or ovary of the adult mouse.…”

Section: Discussionsupporting

confidence: 40%

“…1A) (12,20). By searching for BAC clone DNAs that contained the entire mouse gastrin gene through the Ensemble 129S7-derived mouse genomic BAC database, we found two suitable clones.…”

Section: Generation and Characterization Of Mgas-egfp-bac Transgenementioning

confidence: 99%

“…Studies in the past that have relied primarily on radioimmunoassay of tissue extracts have reported gastrin expression in the organs other than stomach, such as pituitary gland, brain, testis, and ovary (11,12,27,29). Therefore, we investigated the possible presence of GFP expression in these tissues.…”

Section: Generation and Characterization Of Mgas-egfp-bac Transgenementioning

confidence: 99%

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In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice

Takaishi

Shibata

Tomita

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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Gastrin is secreted from a subset of neuroendocrine cells residing in the gastric antrum known as G cells, but low levels are also expressed in fetal pancreas and intestine and in many solid malignancies. Although past studies have suggested that antral gastrin is transcriptionally regulated by inflammation, gastric pH, somatostatin, and neoplastic transformation, the transcriptional regulation of gastrin has not previously been demonstrated in vivo. Here, we describe the creation of an enhanced green fluorescent protein reporter (mGAS-EGFP) mouse using a bacterial artificial chromosome that contains the entire mouse gastrin gene. Three founder lines expressed GFP signals in the gastric antrum and the transitional zone to the corpus. In addition, GFP(+) cells could be detected in the fetal pancreatic islets and small intestinal villi, but not in these organs of the adult mice. The administration of acid-suppressive reagents such as proton pump inhibitor omeprazole and gastrin/CCK-2 receptor antagonist YF476 significantly increased GFP signal intensity and GFP(+) cell numbers in the antrum, whereas these parameters were decreased by overnight fasting, octreotide (long-lasting somatostatin ortholog) infusion, and Helicobacter felis infection. GFP(+) cells were also detected in the anterior lobe of the pituitary gland and importantly in the colonic tumor cells induced by administration with azoxymethane and dextran sulfate sodium salt. This transgenic mouse provides a useful tool to study the regulation of mouse gastrin gene in vivo, thus contributing to our understanding of the mechanisms involved in transcriptional control of the gastrin gene.

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Section: Discussionsupporting

confidence: 40%

“…1A) (12,20). By searching for BAC clone DNAs that contained the entire mouse gastrin gene through the Ensemble 129S7-derived mouse genomic BAC database, we found two suitable clones.…”

Section: Generation and Characterization Of Mgas-egfp-bac Transgenementioning

confidence: 99%

Section: Generation and Characterization Of Mgas-egfp-bac Transgenementioning

confidence: 99%

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In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice

Takaishi

Shibata

Tomita

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…or gastrin (90 nmol kg )1 h )1 ), for strains and numbers see Table 2. The gastrin used was Gastrin I from rat, however mouse and rat gastrin are highly homologous (Friis-Hansen et al 1996).…”

Section: Acid Secretionmentioning

confidence: 99%

An in vivo model for gastric physiological and pathophysiological studies in the mouse

Henriksnäs

Phillipson

Petersson

et al. 2005

Acta Physiologica Scandinavica

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“…Imaging and quantitation were performed on a GS-250 Molecular Imager (BioRad). The gastrin probe was a 422 bp cDNA [20]. After hybridization, filters were stripped and rehybridized with a mouse ribosomal protein L32 probe [21] to control for RNA loading.…”

Section: Rna Analysismentioning

confidence: 99%

Disturbed progastrin processing in carboxypeptidase E‐deficient fat mice

et al. 1997

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The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fatlfat mice by measuring gastrin mRNA, carboxyamidated gastrin and its processing intermediates in the stomach. The tissue concentration of carboxyamidated (i.e. bioactive) gastrin was only slightly reduced (601 ± 28 pmol/g in fatlfat mice vs. 715 ±43 pmol/g in wild-type controls). However, progastrin processing intermediates accumulated excessively with an 86-fold increase in the concentration of the CPE substrate, glycylarginine extended gastrin, and a seven-fold increase in the concentration of glycine-extended gastrin. Accordingly, the total progastrin product was doubled, as was the concentration of gastrin mRNA. Plasma concentrations of carboxyamidated gastrin were, however slightly reduced both in fasted fatlfat mice and postprandially. The results show that the CPE mutation diminishes the efficiency of progastrin processing, but gastrin synthesis is nevertheless increased to maintain an almost normal production of bioactive gastrins. By comparison with other neuroendocrine prohormones, progastrin processing in CPEdeficient mice is unique. Hence, the increase of glycine-extended gastrin in combination with normal levels of carboxyamidated gastrin suggests that G-cells may have another biosynthetic pathway for gastrin. © 1997 Federation of European Biochemical Societies.

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Molecular structure and genetic mapping of the mouse gastrin gene

Cited by 18 publications

References 32 publications

In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice

In vivo analysis of mouse gastrin gene regulation in enhanced GFP-BAC transgenic mice

An in vivo model for gastric physiological and pathophysiological studies in the mouse

Disturbed progastrin processing in carboxypeptidase E‐deficient fat mice

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