Molecular strain typing of <i>Mycobacterium tuberculosis</i> complex in Bamako, Mali

Traoré, Bréhima; Diarra, Bassirou; Dembele, Bindongo P. P.; Somboro, Amadou; Hammond, Abdulrahman S.; Siddiqui, Sophia; Maiga, Mamoudou; Kone, Bourahima; Sarro, Yeya dit Sadio; Washington, J; Parta, Mark; Coulibaly, Nouhoum; Mbaye, O.; Diallo, Souleymane; Koïta, Ousmane; Tounkara, A.; Polis, Michael A.

doi:10.5588/ijtld.11.0397

Cited by 36 publications

(62 citation statements)

References 20 publications

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“…We observed a high proportion of the three subspecies (MTB T1, MAF 2 and MTB LAM), similarly to what Traore et al . reported in 2012, [3,13] suggesting a relative stability and endemicity of the disease transmission. However, unlike Mali, the continuous predominance of MTB T1 was not observed in our neighboring countries such as Burkina Faso, Benin, and Nigeria.…”

Section: Discussionmentioning

confidence: 99%

“…[14] Beyond our West African region, MTB LAM or MTB Cameroon was consistently reported to be the most frequent in Cameroun. [4,15–17] We found eight (1.8%) strains of MTB Beijing with a slight increased proportion compared to Traore et al .’s report of 2012 (1.6%) [3] while a higher prevalence of 10.3% was reported in Benin. [15] The Beijing strain was first described in 2010 in Bamako and continued to be a challenge in our region and globally because of its usual higher resistance to TB drugs.…”

Section: Discussionmentioning

confidence: 99%

“…[1] While the prevalence and incidence rates are decreasing since 2000, there is an increase of drug resistance (DR) within the country mainly the primary MDR-TB which moved from 2.5% in 2011 to 3.4% in 2016. [3,4] Thus, the increased rate of drug-resistant and MDR strains remains a serious concern for TB control in Mali.…”

Section: Introductionmentioning

confidence: 99%

“…reported a predominancy of MTB T1, MTB LAM, and Mycobacterium africanum (MAF) 2 from 126 isolates collected between 2006 and 2010. [3] Thus, the aim of this study was to characterize the global strain diversity and the prevalence of DR profiles in these strains during the past 10 years.…”

Section: Introductionmentioning

confidence: 99%

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The most frequent Mycobacterium tuberculosis complex families in mali (2006–2016) based on spoligotyping

Togo

Kodio

Diarra

et al. 2017

Int J Mycobacteriol

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Objective To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Materials and Methods From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali. Results Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016. Conclusion This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M. tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The most frequent Mycobacterium tuberculosis complex families in mali (2006–2016) based on spoligotyping

Togo

Kodio

Diarra

et al. 2017

Int J Mycobacteriol

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“…Eight clinical isolates of M. tuberculosis with various first-and second-line drug susceptibility patterns (Table 2) were obtained from Project SEREFO-NIAID/University of Bamako Research Collaboration on HIV/TB, in Bamako, Mali, for evaluation in this study (15,16).…”

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confidence: 99%

Efflux Inhibition with Verapamil Potentiates Bedaquiline in Mycobacterium tuberculosis

Gupta

Cohen

Winglee³

et al. 2014

Antimicrob Agents Chemother

143

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Drug efflux is an important resistance mechanism in Mycobacterium tuberculosis. We found that verapamil, an efflux inhibitor, profoundly decreases the MIC of bedaquiline and clofazimine to M. tuberculosis by 8-to 16-fold. This exquisite susceptibility was noted among drug-susceptible and drug-resistant clinical isolates. Thus, efflux inhibition is an important sensitizer of bedaquiline and clofazimine, and efflux may emerge as a resistance mechanism to these drugs.

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Incidence and Drug Resistance of Zoonotic Mycobacterium bovis Infection in Peshawar, Pakistan

Khattak

Mushtaq

Ayaz

et al. 2018

Advances in Experimental Medicine and Biology

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Prevalence of zoonotic Mycobacterium bovis (bTB) disease in human population is underreported from the North of Pakistan. Here, we report on the proportion of human bTB disease among the overall TB patients, drug resistance pattern of bTB isolates, and knowledge, attitude, and practices (KAP)-based analysis of bTB disease. For this purpose, sputum samples from a total of 300 clinically diagnosed TB patients and 100 randomly selected school children suspected of pulmonary TB were processed by culture as well as polymerase chain reaction (PCR) for isolation, identification, and confirmation of Mycobacterium tuberculosis (mTB) and bTB species. Isolates of bTB were processed for drug susceptibility tests. Data on KAP regarding TB were obtained on a pretested questionnaire. Sputum-based PCR results indicated that 288/300 (96%) were confirmed as mTB, while 12/300 (4%) were found as bTB diseases. Interestingly, none of the school child was declared positive for either mTB or bTB. Notably, 274/300 (91.3%) positively cultured samples were identified as mTB, 13/300 (4.3%) as bTB, while 5/300 (1.7%) as mixed containing both. Importantly, except one, all of the bTB isolates were found resistant to pyrazinamide. Surprisingly, most of the bTB isolates (~70%) were found resistant to a broad range of first- and second-line anti-TB drugs. SplitsTree and recombination analysis indicated no evidence of intergenic recombination. Finally, residence, occupation, presence of animals at home, and sleeping alongside animals were found significantly associated with occurrence of bTB disease. To the best of our knowledge, we report for the first time on the high (4%) burden of bTB disease in human TB patients in Peshawar, Pakistan.

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Molecular strain typing of Mycobacterium tuberculosis complex in Bamako, Mali

Abstract: This study indicates a low level of primary drug resistance in Bamako, affirms the importance of using correct drug regimens, and suggests that the MTB T1 strain may be associated with the development of resistance.

Cited by 36 publications

References 20 publications

The most frequent Mycobacterium tuberculosis complex families in mali (2006–2016) based on spoligotyping

The most frequent Mycobacterium tuberculosis complex families in mali (2006–2016) based on spoligotyping

Efflux Inhibition with Verapamil Potentiates Bedaquiline in Mycobacterium tuberculosis

Incidence and Drug Resistance of Zoonotic Mycobacterium bovis Infection in Peshawar, Pakistan

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